Vancomycin-laden calcium phosphate-calcium sulfate composite allows bone formation in a rat infection model

English version

Autoři: K. Keely Boyle ^aff001; Branden Sosa ^aff002; Liza Osagie ^aff003; Kathleen Turajane ^aff002; Mathias P. G. Bostrom ^aff002; Xu Yang ^aff002
Působiště autorů: University at Buffalo, Buffalo, New York, United States of America ^aff001; Hospital for Special Surgery, New York, New York, United States of America ^aff002; Royal Free Hospital, London, England, United Kingdom ^aff003
Vyšlo v časopise: PLoS ONE 14(9)
Kategorie: Research Article
doi: https://doi.org/10.1371/journal.pone.0222034

Souhrn

Objective

Local antibiotic delivery systems with differing chemical and mechanical properties have been developed to assist in the management of osteomyelitis. We investigated the bone conductive and resorptive capabilities of a calcium phosphate-calcium sulfate (CaP/CaS) composite compared with commercially available polymethylmethacrylate (PMMA). In addition, we compared the in vivo preventative and treatment efficacies of both biomaterials in a proven osteomyelitis model.

Methods

Sixty-four, male Sprague-Dawley rats were inoculated with 10 μl of 1.5 x 10⁸ CFU/ml of Staphylococcus aureus in a surgically drilled defect in the right proximal tibia. Infected animals were randomly allocated into prevention and treatment groups with 32 rats each. In the prevention group, the defect was filled with a plug containing either PMMA or CaP/CaS immediately after the inoculation. In the treatment group, the infected defects were irrigated, debrided, and filled with either a PMMA or CaP/CaS plug. Both CaP/CaS and PMMA were impregnated with 10% weight of vancomycin. Rats were sacrificed 6 weeks after cement insertion. Infection was detected by bacterial culture and histological analysis. Bone formation in the defect was assessed with micro-computed tomography and histology.

Results

No bacteria were detected in any group. Both the prevention and treatment groups using CaP/CaS had significantly more bone volume fraction, bone area, and cartilage area than the PMMA groups.

Conclusions

When loaded with 10% of vancomycin, CaP/CaS and PMMA have the same efficacy for treatment and prevention of osteomyelitis. CaP/CaS enhances bone defect healing through improved bone remodeling in our osteomyelitis rat model.

Klíčová slova:

Medicine and health sciences – Infectious diseases – Bacterial diseases – Osteomyelitis – Rheumatology – Connective tissue diseases – Pharmacology – Drugs – Vancomycin – Critical care and emergency medicine – Trauma medicine – Traumatic injury – Bone fracture – Skeleton – Biology and life sciences – Microbiology – Microbial control – Antimicrobials – Antibiotics – Anatomy – Musculoskeletal system – Physical sciences – Chemistry – Chemical compounds – Salts – Sulfates – Materials science – Materials – Composite materials – Binders – Cements

Zdroje

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Vancomycin-laden calcium phosphate-calcium sulfate composite allows bone formation in a rat infection model

Souhrn

Objective

Methods

Results

Conclusions

Klíčová slova:

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