Drought mediated physiological and molecular changes in muskmelon (Cucumis melo L.)

English version

Autoři: Waquar Akhter Ansari ^aff001; Neelam Atri ^aff002; Javed Ahmad ^aff003; Mohammad Irfan Qureshi ^aff003; Bijendra Singh ^aff001; Ram Kumar ^aff004; Vandna Rai ^aff004; Sudhakar Pandey ^aff001
Působiště autorů: ICAR–Indian Institute of Vegetable Research, Varanasi, Uttar Pradesh, India ^aff001; Department of Botany, M.M.V., Banaras Hindu University, Varanasi, Uttar Pradesh, India ^aff002; Proteomics & Bioinformatics Lab, Department of Biotechnology, Jamia Millia Islamia, New Delhi, India ^aff003; ICAR–National Research Centre on Plant Biotechnology, LBS Centre, Pusa Campus, New Delhi, India ^aff004
Vyšlo v časopise: PLoS ONE 14(9)
Kategorie: Research Article
doi: https://doi.org/10.1371/journal.pone.0222647

Souhrn

Water deficiency up to a certain level and duration leads to a stress condition called drought. It is a multi-dimensional stress causing alteration in the physiological, morphological, biochemical, and molecular traits in plants resulting in improper plant growth and development. Drought is one of the major abiotic stresses responsible for loss of crops including muskmelon (Cucumis melo. L). Muskmelon genotype SC-15, which exhibits high drought resistance as reported in our earlier reports, was exposed to deficient water condition and studied for alteration in physiological, molecular and proteomic profile changes in the leaves. Drought stress results in reduced net photosynthetic rate (Pn), stomatal conductance (Gs) and transpiration (E) rate. With expanded severity of drought, declination recorded in content of total chlorophyll and carotenoid while enhancement observed in phenol content indicating generation of oxidative stress. In contrary, activities of catalase (CAT), superoxide dismutase (SOD), ascorbate peroxidase (APX), and guaiacol (POD) were increased under drought stress. Peptide mass fingerprinting (PMF) showed that drought increased the relative abundance of 38 spots while decreases10 spots of protein. The identified proteins belong to protein synthesis, photosynthesis, nucleotide biosynthesis, stress response, transcription regulation, metabolism, energy and DNA binding. A drought-induced MADS-box transcription factor was identified. The present findings indicate that under drought muskmelon elevates the abundance of defense proteins and suppresses catabolic proteins. The data obtained exhibits possible mechanisms adopted by muskmelon to counter the impacts of drought induced stress.

Klíčová slova:

DNA-binding proteins – Chlorophyll – Leaves – Plant resistance to abiotic stress – Protein expression – Transcription factors – Transcriptional control – Drought

Zdroje

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Drought mediated physiological and molecular changes in muskmelon (Cucumis melo L.)

Souhrn

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