Genetically distinct Group B Streptococcus strains induce varying macrophage cytokine responses
Autoři:
Rebecca A. Flaherty aff001; Elena C. Borges aff001; Jessica A. Sutton aff002; David M. Aronoff aff003; Jennifer A. Gaddy aff003; Margaret G. Petroff aff001; Shannon D. Manning aff001
Působiště autorů:
Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, MI, United States of America
aff001; Department of Microbiology and Immunology, Meharry Medical College School of Medicine, Nashville, TN, United States of America
aff002; Department of Medicine, Division of Infectious Disease, Vanderbilt University Medical Center, Nashville, TN, United States of America
aff003; Pathobiology and Diagnostic Investigation, Michigan State University, East Lansing, MI, United States of America
aff004
Vyšlo v časopise:
PLoS ONE 14(9)
Kategorie:
Research Article
doi:
https://doi.org/10.1371/journal.pone.0222910
Souhrn
Group B Streptococcus (GBS) is an opportunistic pathogen that causes preterm birth and neonatal disease. Although GBS is known to exhibit vast diversity in virulence across strains, the mechanisms of GBS-associated pathogenesis are incompletely understood. We hypothesized that GBS strains of different genotypes would vary in their ability to elicit host inflammatory responses, and that strains associated with neonatal disease would induce different cytokine profiles than those associated with colonization. Using a multiplexed, antibody-based protein detection array, we found that production of a discrete number of inflammatory mediators by THP-1 macrophage-like cells was universally induced in response to challenge with each of five genetically distinct GBS isolates, while other responses appeared to be strain-specific. Key array responses were validated by ELISA using the initial five strains as well as ten additional strains with distinct genotypic and phenotypic characteristics. Interestingly, IL-6 was significantly elevated following infection with neonatal infection-associated sequence type (ST)-17 strains and among strains possessing capsule (cps) type III. Significant differences in production of IL1-β, IL-10 and MCP-2 were also identified across STs and cps types. These data support our hypothesis and suggest that unique host innate immune responses reflect strain-specific differences in virulence across GBS isolates. Such data might inform the development of improved diagnostic or prognostic strategies against invasive GBS infections.
Klíčová slova:
Biology and life sciences – Physiology – Developmental biology – Molecular development – Neonates – Cell biology – Cellular types – Animal cells – Blood cells – White blood cells – Macrophages – Immune cells – Medicine and health sciences – Immune physiology – Immunology – Immune system – Innate immune system – Cytokines – Immune response – Inflammation – Diagnostic medicine – Signs and symptoms – Sepsis – Neonatal sepsis – Pathology and laboratory medicine – Research and analysis methods – Immunologic techniques – Immunoassays – Enzyme-linked immunoassays
Zdroje
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