Irisin promotes C2C12 myoblast proliferation via ERK-dependent CCL7 upregulation
Autoři:
Jangho Lee aff001; Joon Park aff001; Young Ho Kim aff003; Nam Hyouck Lee aff003; Kyung-Mo Song aff003
Působiště autorů:
Research Division of Food Functionality, Korea Food Research Institute, Iseo-myeon, Wanju-gun, Jeollabuk-do, Republic of Korea
aff001; Department of Food Biotechnology, Korea University of Science and Technology, Daejeon, Republic of Korea
aff002; Research Division of Strategic Food Technology, Korea Food Research Institute, Iseo-myeon, Wanju-gun, Jeollabuk-do, Republic of Korea
aff003
Vyšlo v časopise:
PLoS ONE 14(9)
Kategorie:
Research Article
doi:
https://doi.org/10.1371/journal.pone.0222559
Souhrn
Irisin is an exercise-induced myokine that has various physiological functions, such as roles in energy expenditure, glucose/lipid metabolism, and muscle development. In muscle development, myoblast proliferation is known to be a first step, and recent studies have reported that an increased irisin level is involved in the promotion of cell proliferation in various cell types, including myoblasts. However, the exact mechanism of action by which irisin promotes myoblast proliferation has not been reported. In this study, we aimed to determine the pro-proliferative effect of irisin on C2C12 myoblasts and its mechanism of action. Irisin induced C2C12 cell proliferation and upregulated the mRNA levels of markers of proliferation Pcna, Mki67, and Mcm2. Irisin increased extracellular signal-regulated kinase (ERK) phosphorylation, and U0126, an ERK pathway inhibitor, suppressed irisin-induced C2C12 cell proliferation. Transcriptomic and qRT-PCR analysis showed that Ccl2, Ccl7, Ccl8, and C3 are potential downstream regulators of ERK signaling that promote C2C12 cell proliferation. Knockdown of Ccl7 revealed that irisin upregulates chemokine (C-C motif) ligand 7 (CCL7) and subsequently promotes C2C12 cell proliferation. These results suggest that irisin promotes C2C12 myoblast proliferation via ERK-dependent CCL7 upregulation and may aid in understanding how irisin contributes to muscle development.
Klíčová slova:
Biology and life sciences – Cell biology – Cell processes – Cell proliferation – Cellular types – Animal cells – Stem cells – Myoblasts – Signal transduction – Cell signaling – Signaling cascades – ERK signaling cascade – Genetics – Gene expression – Gene regulation – Small interfering RNAs – Genomics – Genome analysis – Transcriptome analysis – Biochemistry – Nucleic acids – RNA – Non-coding RNA – Computational biology – Medicine and health sciences – Immunology – Immune response – Research and analysis methods – Bioassays and physiological analysis – Biochemical analysis – Colorimetric assays – MTS assay – Enzyme assays
Zdroje
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Článek vyšel v časopise
PLOS One
2019 Číslo 9
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