Sensitivity of the fasciae to sex hormone levels: Modulation of collagen-I, collagen-III and fibrillin production

English version

Autoři: Caterina Fede ^aff001; Carmelo Pirri ^aff002; Chenglei Fan ^aff001; Giovanna Albertin ^aff001; Andrea Porzionato ^aff001; Veronica Macchi ^aff001; Raffaele De Caro ^aff001; Carla Stecco ^aff001
Působiště autorů: Department of Neurosciences, Institute of Human Anatomy, University of Padova, Padova, Italy ^aff001; Physical and Rehabilitation Medicine, University of Rome “Tor Vergata”, Roma, Italy ^aff002
Vyšlo v časopise: PLoS ONE 14(9)
Kategorie: Research Article
doi: https://doi.org/10.1371/journal.pone.0223195

Souhrn

Although it is now recognized that women suffer from myofascial pain to a greater extent than men, and that the muscular fasciae can respond to hormonal stimuli, thanks to the expression of sex hormone receptors, how the fasciae can modify their structure under hormonal stimulation is not clear. In this work, an immunocytochemical analysis of collagen-I, collagen-III and fibrillin were carried out on fibroblasts isolated from human fascia lata after in vitro treatment with various levels of sex hormones β-estradiol and/or relaxin-1, according to the phases of a woman’s period (follicular, periovulatory, luteal, post-menopausal phases and pregnancy). This study demonstrates for the first time that fascial cells can modulate the production of some components of the extracellular matrix according to hormone levels, when treated with β-estradiol: collagen-I falls from 6% of positivity in the follicular phase to 1.9 in the periovulatory phase. However, after the addition of relaxin-1 to the cell culture, the production of extracellular matrix decreased and remained at the same level (1.7% of collagen-I, at both follicular and periovulatory levels of hormones). These results confirm the antifibrotic function of relaxin-1, thanks to its ability to reduce matrix synthesis. They are also a first step in our understanding of how some hormonal dysfunctions in women can cause a dysregulation of extracellular matrix production in fasciae.

Klíčová slova:

Cell staining – Collagens – Extracellular matrix – Fibroblasts – Hormones – Pregnancy – Sex hormones – Estrogens

Zdroje

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Sensitivity of the fasciae to sex hormone levels: Modulation of collagen-I, collagen-III and fibrillin production

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