Verification of hub genes in the expression profile of aortic dissection
Autoři:
Weitie Wang aff001; Qing Liu aff002; Yong Wang aff001; Hulin Piao aff001; Bo Li aff001; Zhicheng Zhu aff001; Dan Li aff001; Tiance Wang aff001; Rihao Xu aff001; Kexiang Liu aff001
Působiště autorů:
Department of Cardiovascular Surgery, Second Hospital of Jilin University, Changchun, Jilin, China
aff001; Graduate School of Medicine and Faculty of Medicine, University of Tokyo, Tokyo, Japan
aff002
Vyšlo v časopise:
PLoS ONE 14(11)
Kategorie:
Research Article
doi:
https://doi.org/10.1371/journal.pone.0224922
Souhrn
Background
To assess the mRNA expression profile and explore the hub mRNAs and potential molecular mechanisms in the pathogenesis of human thoracic aortic dissection (TAD).
Methodology
mRNA microarray expression signatures of TAD tissues (n = 6) and non-TAD tissues (NT; n = 6) were analyzed by an Arraystar human mRNA microarray. Real-time PCR (qRT-PCR) was used to validate the results of the mRNA microarray. Bioinformatic tools, including Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analysis, were utilized. Protein-protein interaction (PPI) networks were constructed based on data from the STRING database. Molecular Complex Detection (MCODE) and cytoHubba analyses were used to predict the strongest hub gene and pathway.
Results
The top 10 hub genes were CDK1, CDC20, CCNB2, CCNB1, MAD2L1, AURKA, C3AR1, NCAPG, CXCL12 and ASPM, which were identified from the PPI network. Module analysis revealed that TAD was associated with the cell cycle, oocyte meiosis, the p53 signaling pathway, and progesterone-mediated oocyte maturation. The qRT-PCR results showed that the expression of all hub genes was significantly increased in TAD samples (p < 0.05). Immunostaining of Ki-67 and CDK1 showed a high proliferation state and high expression in TAD, respectively.
Conclusions
CDK1 could be used as a potential diagnostic biomarker and therapeutic target of TAD.
Klíčová slova:
Binding analysis – Cell cycle and cell division – Cellular structures and organelles – Cytoskeletal proteins – Gene expression – Immune response – Microarrays – Muscle contraction
Zdroje
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