Real-time telemetry monitoring of oxygen in the central complex of freely-walking Gromphadorhina portentosa

English version

Autoři: Pier Andrea Serra ^aff001; Paola Arrigo ^aff001; Andrea Bacciu ^aff001; Daniele Zuncheddu ^aff001; Riccardo Deliperi ^aff001; Diego Antón Viana ^aff001; Patrizia Monti ^aff001; Maria Vittoria Varoni ^aff004; Maria Alessandra Sotgiu ^aff005; Pasquale Bandiera ^aff005; Gaia Rocchitta ^aff001
Působiště autorů: Department of Medical, Surgical and Experimental Medicine, Medical School, University of Sassari, Sassari, Italy ^aff001; Institute of Sciences of Food Production, Italian National Research Council, Sassari, Italy ^aff002; Mediterranean Center for Disease Control, University of Sassari, Sassari, Italy ^aff003; Department of Veterinary Medicine, Medical School, University of Sassari, Sassari, Italy ^aff004; Department of Biomedical Sciences, Medical School, University of Sassari, Sassari, Italy ^aff005
Vyšlo v časopise: PLoS ONE 14(11)
Kategorie: Research Article
doi: https://doi.org/10.1371/journal.pone.0224932

Souhrn

A new telemetric system for the electrochemical monitoring of dissolved oxygen is showed. The device, connected with two amperometric sensors, has been successfully applied to the wireless detection of the extracellular oxygen in the central complex of freely-walking Gromphadorhina portentosa. The unit was composed of a potentiostat, a two-channel sensor conditioning circuit, a microprocessor module, and a wireless serial transceiver. The amperometric signals were digitalized and sent to a notebook using a 2.4 GHz transceiver while a serial-to-USB converter was connected to a second transceiver for completing the communication bridge. The software, running on the laptop, allowed to save and graph the oxygen signals. The electronics showed excellent stability and the acquired data was linear in a range comprised between 0 and -165 nA, covering the entire range of oxygen concentrations. A series of experiments were performed to explore the dynamics of dissolved oxygen by exposing the animals to different gases (nitrogen, oxygen and carbon dioxide), to low temperature and anesthetic agents (chloroform and triethylamine). The resulting data are in agreement with previous O₂ changes recorded in the brain of awake rats and mice. The proposed system, based on simple and inexpensive components, can constitute a new experimental model for the exploration of central complex neurochemistry and it can also work with oxidizing sensors and amperometric biosensors.

Klíčová slova:

Anesthetics – Carbon dioxide – Glucose metabolism – Insects – Oxygen – Oxygen metabolism – Cockroaches – Chloroform

Zdroje

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Real-time telemetry monitoring of oxygen in the central complex of freely-walking Gromphadorhina portentosa

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