The calcium sensor OsCBL1 modulates nitrate signaling to regulate seedling growth in rice
Autoři:
Jing Yang aff001; Xiaolong Deng aff001; Xiaoxin Wang aff001; Jingzhang Wang aff001; Shiyun Du aff002; Yangsheng Li aff001
Působiště autorů:
State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan, P. R. China
aff001; Institute of Rice Research, Anhui Academy of Agricultural Sciences, Hefei, P. R. China
aff002
Vyšlo v časopise:
PLoS ONE 14(11)
Kategorie:
Research Article
doi:
https://doi.org/10.1371/journal.pone.0224962
Souhrn
Nitrate signaling integrates and coordinates gene expression and plant growth; however, the underlying molecular mechanisms involved remain poorly understood. Our previous study revealed that rice calcineurin B-like protein 1 (OsCBL1) modulates lateral root elongation by affecting auxin biosynthesis. Here, we report that OsCBL1 also modulates nitrate signaling to regulate rice seedlings growth. Compared with wild-type seedlings, seedlings of OsCBL1-knockdown (OsCBL1-KD) plants showed a suppressed growth phenotype, which included reduced root and shoot fresh weights and shorter radicles, crown roots, and lateral roots, when grown in nitrogen-free conditions. Although the growth defects of OsCBL1-KD plants could be partially rescued by the addition of nitrate to the growth conditions, the nitrate uptake capability of the OsCBL1-KD plants did not differ from that of wild-type plants as assessed via nitrate content and 15NO3− influx experiments. The nitrate-regulated expression of nitrate signal sentinel genes (OsNRT2.1 and OsNRT2.2) was affected in the OsCBL1-KD plants under both long- and short-term nitrate treatments. Overall, our results showed a novel role for OsCBL1 in the regulation of nitrate signaling and nitrate-mediated rice growth.
Klíčová slova:
Calcium signaling – Gene expression – Gene regulation – Nitrates – Rice – Root growth – Seedlings – Protein kinase signaling cascade
Zdroje
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PLOS One
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