Antibacterial efficacy of cold atmospheric plasma against Enterococcus faecalis planktonic cultures and biofilms in vitro

English version

Autoři: Felix Theinkom ^aff001; Larissa Singer ^aff001; Fabian Cieplik ^aff002; Sylvia Cantzler ^aff003; Hannes Weilemann ^aff003; Maximilian Cantzler ^aff003; Karl-Anton Hiller ^aff002; Tim Maisch ^aff001; Julia L. Zimmermann ^aff004
Působiště autorů: Department of Dermatology, University Hospital Regensburg, Regensburg, Germany ^aff001; Department of Conservative Dentistry and Periodontology, University Hospital Regensburg, Regensburg, Germany ^aff002; terraplasma GmbH, Garching, Germany ^aff003; terraplasma medical GmbH, Garching, Germany ^aff004
Vyšlo v časopise: PLoS ONE 14(11)
Kategorie: Research Article
doi: https://doi.org/10.1371/journal.pone.0223925

Souhrn

Nosocomial infections have become a serious threat in our times and are getting more difficult to handle due to increasing development of resistances in bacteria. In this light, cold atmospheric plasma (CAP), which is known to effectively inactivate microorganisms, may be a promising alternative for application in the fields of dentistry and dermatology. CAPs are partly ionised gases, which operate at low temperature and are composed of electrons, ions, excited atoms and molecules, reactive oxygen and nitrogen species. In this study, the effect of CAP generated from ambient air was investigated against Enterococcus faecalis, grown on agar plates or as biofilms cultured for up to 72 h. CAP reduced the colony forming units (CFU) on agar plates by > 7 log₁₀ steps. Treatment of 24 h old biofilms of E. faecalis resulted in CFU-reductions by ≥ 3 log₁₀ steps after CAP treatment for 5 min and by ≥ 5 log₁₀ steps after CAP treatment for 10 min. In biofilm experiments, chlorhexidine (CHX) and UVC radiation served as positive controls and were only slightly more effective than CAP. There was no damage of cytoplasmic membranes upon CAP treatment as shown by spectrometric measurements for release of nucleic acids. Thus, membrane damage seems not to be the primary mechanism of action for CAP towards E. faecalis. Overall, CAP showed pronounced antimicrobial efficacy against E. faecalis on agar plates as well as in biofilms similar to positive controls CHX or UVC.

Klíčová slova:

Antimicrobials – Bacterial biofilms – Biofilm culture – Biofilms – Enterococcus faecalis – Nucleic acids – Ultraviolet C – Plasmas

Zdroje

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Antibacterial efficacy of cold atmospheric plasma against Enterococcus faecalis planktonic cultures and biofilms in vitro

Souhrn

Klíčová slova:

Zdroje

PLOS One

INSIGHTS from European Respiratory Congress

Současné pohledy na riziko v parodontologii

Svět praktické medicíny 3/2024 (znalostní test z časopisu)

Kardiologické projevy hypereozinofilií

Střevní příprava před kolonoskopií