Artificial insemination with fresh, liquid stored and frozen thawed semen in dromedary camels
Autoři:
Samir Al-Bulushi aff001; Bodhaganahalli M. Manjunatha aff002; Roslyn Bathgate aff003; Jessica P. Rickard aff001; Simon P. de Graaf aff001
Působiště autorů:
The University of Sydney, Faculty of Science, School of Life and Environmental Sciences, NSW, Australia
aff001; Animal Research Centre, Directorate General of Veterinary Services, Royal Court Affairs, Muscat, Oman
aff002; The University of Sydney, Faculty of Science, Sydney School of Veterinary Science, NSW, Australia
aff003
Vyšlo v časopise:
PLoS ONE 14(11)
Kategorie:
Research Article
doi:
https://doi.org/10.1371/journal.pone.0224992
Souhrn
This study was conducted to evaluate various factors affecting fertility following insemination of dromedary camels. In experiment 1, camels were either bred by natural mating (NM) or inseminated in the body of uterus with whole, split (50:50) or 1 mL of undiluted ejaculate. In experiment 2, camels were inseminated with fresh diluted semen either in the body of the uterus or tip of the uterine horn and at either the time of ovulation induction (0 h), 24 or 30 h later. In experiment 3, camels were inseminated at the tip of the uterine horn with different doses of fresh diluted semen (75, 150 or 300 x 106 motile spermatozoa) or with 150 x 106 motile spermatozoa diluted with different extenders (Green buffer, Optixcell or Triladyl). In experiment 4, camels were inseminated in the tip of the uterine horn with diluted (Triladyl or Optixcell) liquid-stored semen or diluted (Triladyl) frozen-thawed semen consisting of either 300 or 500 x 106 motile spermatozoa. The pregnancy rate in camels bred by NM was similar to camels inseminated with whole undiluted ejaculates whereas insemination with 1 mL undiluted ejaculate resulted in lower pregnancy compared to whole and split undiluted ejaculates (P < 0.05). Deposition of semen in the uterine body resulted in lower pregnancy rates compared to deposition in the tip of the horn (35.3% versus 72.2%, P < 0.05) but insemination at the time of ovulation induction and 24 h later resulted in higher pregnancy rate to camels inseminated at 30 h after induction (68.4 and 70.0% versus 23.5%; P < 0.05). Artificial insemination with 75 x 106 motile spermatozoa resulted in lower pregnancy rates compared to 150 and 300 x 106 motile spermatozoa doses (40.9% versus 65.2 and 70.0%, respectively) and pregnancy rate was not affected by extenders. Insemination of chilled motile spermatozoa stored in either Triladyl or Optixcell resulted in similar pregnancy rates, regardless of insemination dose, although an upward trend with increasing sperm number was apparent (Triladyl; 11.1% versus 21.1% and Optixcell; 5.9% versus 12.5%, for 300 x 106 and 500 x 106 groups, respectively; P > 0.05). No pregnancies were obtained with frozen thawed semen. In conclusion, this study demonstrated that the success of camel AI is highly dependent on sperm dose, location of semen deposition, timing of insemination and semen type. Further studies are required to determine the reason for the compromised fertility of preserved semen despite apparent high in vitro quality.
Klíčová slova:
Acrosomes – Camels – Pregnancy – Semen – Sperm – Uterus – Insemination – Ovulation
Zdroje
1. Elliot F. Artificial insemination of a bactrian camel (Camelus bactrianus). International Zoo Year book. 1961;3:94–5.
2. Tibary A, Anouassi A. Artificial breeding and manipulation of reproduction in camelidae. In: Tibary A, editor. Theriogenology in camelidae: anatomy, physiology, pathology and artificial breeding. Institute Agronomique et Veterinaire Hassan II, Rabat, Marocco: Abu Dhabi Printing and Publishing Company, U.A.E; 1997. p. 413–57.
3. Skidmore J, Morton K, Billah M. Artificial insemination in dromedary camels. Animal Reproduction Science. 2013;136(3):178–86. doi: 10.1016/j.anireprosci.2012.10.008 23153625
4. Deen A, Vyas S, Sahani M. Semen collection, cryopreservation and artificial insemination in the dromedary camel. Animal Reproduction Science. 2003;77(3):223–33.
5. El-Hassanein E. An invention for easy semen collection from dromedary camels, El-Hassanein camel dummy. In: S J.A, Adams GP, editors. Recent Advances in Camel Reproduction: International Veterinary Information Service; 2003. p. 0203.
6. Ziapour S, Niasari-Naslaji A, Mirtavousi M, Keshavarz M, Kalantari A, Adel H. Semen collection using phantom in dromedary camel. Animal Reproduction Science. 2014;151(1):15–21.
7. Skidmore J, Malo C, Crichton E, Morrell J, Pukazhenthi B. An update on semen collection, preservation and artificial insemination in the dromedary camel (Camelus dromedarius). Animal Reproduction Science. 2018;194:11–8. doi: 10.1016/j.anireprosci.2018.03.013 29572045
8. Morton K, Billah M, Skidmore J. Effect of sperm diluent and dose on the pregnancy rate in dromedary camels after artificial insemination with fresh and liquid-stored semen. Journal of Camelid Science. 2013;6:49–62.
9. Musa B, Sieme H, Merkt H, Hago B, editors. Artificial insemination in dromedary camels. The First International Camel Conference; 1992; Newmarket, UK.
10. Medan MS, Absy G, Zeidan AE, Khalil MH, Khalifa HH, Abdel-Salaam AM, et al. Survival and fertility rate of cooled dromedary camel spermatozoa supplemented with catalase enzyme. Journal of Reproduction and Development. 2008;54(1):84–9. doi: 10.1262/jrd.19124 18094528
11. Manjunatha B, Al-Bulushi S, Pratap N. Synchronisation of the follicular wave with GnRH and PGF 2α analogue for a timed breeding programme in dromedary camels (Camelus dromedarius). Animal Reproduction Science. 2015;160:23–9. doi: 10.1016/j.anireprosci.2015.06.023 26164524
12. Manjunatha B, Al-Hosni A, Al-Bulushi S. Resynchronization of synchronized follicular wave in dromedary camels of unknown pregnancy status (Camelus dromedarius). Theriogenology. 2018;119:208–13. doi: 10.1016/j.theriogenology.2018.07.001 30031367
13. Anouassi A, Adnani M, Raed E. Artificial insemination in the camel requires induction of ovulation to achieve pregnancy. In: Allen W, Higgins A, Mayhew I, Snow D, JF W, editors. The First International Camel Conference; Newmarket, UK: R&W Publications; 1992. p. 175–8.
14. Skidmore J, editor Reproductive physiology in male and female camels. Recent Advances in Camelid Reproduction 2000; Ithaca, New York: International Veterinary Information Service.
15. Skidmore J, Billah M. Comparison of pregnancy rates in dromedary camels (Camelus dromedarius) after deep intra-uterine versus cervical insemination. Theriogenology. 2006;66(2):292–6. doi: 10.1016/j.theriogenology.2005.11.013 16356542
16. Morton K, Billah M, Skidmore J. Effect of green buffer storage on the fertility of fresh camel semen after artificial insemination. Reproduction in Domestic Animals. 2011;46(3):554–7. doi: 10.1111/j.1439-0531.2010.01678.x 21545653
17. Skidmore J, Billah M, editors. Investigation of the most appropriate time for insemination of female camels (Camelus dromedarius) after GnRH injection and comparison of pregnancy rates after deep intra-uterine versus cervical insemination. Proceedings of First Conference of International Society of Camelids Research and Development (ISOCARD); 2006; Al-Ain, United Arab Emirates.
18. Anouassi A, Tibary A. Effect of volume and timing of induction of ovulation on conception rate following deep horn insemination in camels (Camelus dromedarius). Clinical Theriogenology. 2010;2:391.
19. Zhao X, Huang Y, Chen B, editors. Artificial insemination with deep-frozen semen in the Bactrian camel (Camelus bactrianus). The First China International Annual Meeting on Agricultural Science and Technology; 1996; Beijing: Chinese Agriculture Press.
20. Akbar S, Hassan S, Ahmad M. Studies on semen processing, cryopreservation and artificial insemination in dromedary camel. International Journal of Animal Science. 2018;2(2):1018.
21. Al-Bulushi S, Manjunatha B, Bathgate R, Rickard J, de Graaf S. Effect of semen collection frequency on the semen characteristics of dromedary camels. Animal Reproduction Science. 2018;197:145–53. doi: 10.1016/j.anireprosci.2018.08.022 30170879
22. Evans G, Maxwell WC. Handling and examination of semen. In: Evans G, Maxwell WC, editors. Salamons' artificial insemination of sheep and goats. 1. Sydney: Butterworths; 1987. p. 93–106.
23. Kershaw-Young C, Stuart C, Evans G, Maxwell W. The effect of glycosaminoglycan enzymes and proteases on the viscosity of alpaca seminal plasma and sperm function. Animal Reproduction Science. 2013;138(3):261–7.
24. Manjunatha B, Al-Bulushi S, Pratap N. Characterization of ovulatory capacity development in the dominant follicle of dromedary camels (Camelus dromedarius). Reproductive Biology. 2015;15(3):188–91. doi: 10.1016/j.repbio.2015.06.002 26370463
25. Calderon W, Sumar J, Franco E. Avances en la Inseminacion Artificial de las alpacas (Lama pacos). Revista de la Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima, Peru. 1968;22:19–35.
26. Sieme H, Merkt H, Musa B, Badreldin H, Willmen T, editors. Liquid and deep freeze preservation of camel semen using different extenders and methods. Proceeding of Workshop “Is it possible to improve the Reproductive performance of the Camel?”; 1990; Paris.
27. Waheed M, Ghoneim I, Al-Eknah M, Al-Haider A. Effect of extenders on motility and viability of chilled-stored camel spermatozoa (Camelus dromedarius). Journal of Camel Practice and Research. 2010;17(2):217–20.
28. Al-Bulushi S, Manjunatha B, Bathgate R, de Graaf S, editors. Effect of different extenders on sperm motion characteristics, viability and acrosome integrity during liquid storage of dromedary camel semen. Animal Reproduction Science; 2016; Tours, France.
29. Vaughan J, Galloway D, Hopkins D. Artificial insemination in alpacas (Lama pacos). Rural Industries Research and Development Corporation, Kingston, ACT, Australia. 2003;1:60–6.
30. Giuliano S, Chaves M, Trasorras V, Gambarotta M, Neild D, Director A, et al. Development of an artificial insemination protocol in llamas using cooled semen. Animal Reproduction Science. 2012;131(3–4):204–10. doi: 10.1016/j.anireprosci.2012.03.010 22503638
Článek vyšel v časopise
PLOS One
2019 Číslo 11
- S diagnostikou Parkinsonovy nemoci může nově pomoci AI nástroj pro hodnocení mrkacího reflexu
- Je libo čepici místo mozkového implantátu?
- Pomůže v budoucnu s triáží na pohotovostech umělá inteligence?
- AI může chirurgům poskytnout cenná data i zpětnou vazbu v reálném čase
- Nová metoda odlišení nádorové tkáně může zpřesnit resekci glioblastomů
Nejčtenější v tomto čísle
- A daily diary study on maladaptive daydreaming, mind wandering, and sleep disturbances: Examining within-person and between-persons relations
- A 3’ UTR SNP rs885863, a cis-eQTL for the circadian gene VIPR2 and lincRNA 689, is associated with opioid addiction
- A substitution mutation in a conserved domain of mammalian acetate-dependent acetyl CoA synthetase 2 results in destabilized protein and impaired HIF-2 signaling
- Molecular validation of clinical Pantoea isolates identified by MALDI-TOF
Zvyšte si kvalifikaci online z pohodlí domova
Všechny kurzy