Nitrogen nutrition is a key modulator of the sugar and organic acid content in citrus fruit

English version

Autoři: Ling Liao ^aff001; Tiantian Dong ^aff001; Xia Qiu ^aff001; Yi Rong ^aff001; Zhihui Wang ^aff001; Jin Zhu ^aff003
Působiště autorů: College of Horticulture, Sichuan Agricultural University, Chengdu, China ^aff001; Institute of Pomology and Olericulture, Sichuan Agricultural University, Chengdu, China ^aff002; Sichuan Provincial Agricultural Department, Chengdu, China ^aff003
Vyšlo v časopise: PLoS ONE 14(10)
Kategorie: Research Article
doi: https://doi.org/10.1371/journal.pone.0223356

Souhrn

‘Huangguogan’ (Citrus reticulata × C. sinensis) is a new cultivar of mandarin citrus in China, and the research on fertilization of ‘Huangguogan’ is very limited. In this study, the effect of N fertilization on ‘Huangguogan’ fruit quality was determined at ripening. Sugars (sucrose, fructose, and glucose), organic acids (pyruvic, oxalic, citric acid, etc.), and vitamin components were measured at six stages of fruit development, and eight enzymes related to the glycolytic and Krebs cycle were assessed. The 1.81 kg N y^-1 treatment group showed the highest total soluble solids concentration and total soluble solids/titratable acidity ratio but the lowest titratable acidity (acid content) at ripening, while the N₁ treatment (0 kg N y^-1) showed the opposite trend. Sucrose and citric acid accumulated to the largest extent during fruit development. Sucrose and ascorbic acid content increased (8.46 to 50.97 mg g^-1 and 8.16 to 27.39 mg g^-1, respectively), while citric acid content decreased (90.81 to 0.02 mg g^-1). Aconitase was the key enzyme responsible for the observed changes in citric acid. The N concentrations in ripening fruit ranged from 2.25% to 4.15%. Curve estimation and principal component analysis revealed that fruit N was positively correlated with the sugars and vitamin components and negatively correlated with the organic acids. The accumulation of these metabolites seemed closely related to the dynamic changes in fruit N concentration at the five N levels tested. In conclusion, we suggest that the 1.81 kg N y^-1 treatment represents the most suitable N fertilizer treatment for ‘Huangguogan’ citrus fruit.

Klíčová slova:

Citrus – Enzyme metabolism – Fertilizers – Fruits – Sucrose – vitamin C – Organic acids – Citric acid

Zdroje

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Nitrogen nutrition is a key modulator of the sugar and organic acid content in citrus fruit

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