Comparative genomic analyses reveal diverse virulence factors and antimicrobial resistance mechanisms in clinical Elizabethkingia meningoseptica strains

English version

Autoři: Shicheng Chen ^aff001; Marty Soehnlen ^aff002; Jochen Blom ^aff003; Nicolas Terrapon ^aff004; Bernard Henrissat ^aff004; Edward D. Walker ^aff001
Působiště autorů: Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, MI, United States of America ^aff001; Michigan Department of Health and Human Services, Bureau of Laboratories, Lansing, MI, United States of America ^aff002; Bioinformatics and Systems Biology, Justus-Liebig-University, Giessen, Germany ^aff003; Architecture et Fonction des Macromolécules Biologiques, Centre National de la Recherche Scientifique (CNRS), Aix-Marseille Université (AMU), UMR 7257, Marseille, France ^aff004; Institut National de la Recherche Agronomique (INRA), USC 1408 AFMB, Marseille, France ^aff005; Department of Biological Sciences, King Abdulaziz University, Jeddah, Saudi Arabia ^aff006
Vyšlo v časopise: PLoS ONE 14(10)
Kategorie: Research Article
doi: https://doi.org/10.1371/journal.pone.0222648

Souhrn

Three human clinical isolates of bacteria (designated strains Em1, Em2 and Em3) had high average nucleotide identity (ANI) to Elizabethkingia meningoseptica. Their genome sizes (3.89, 4.04 and 4.04 Mb) were comparable to those of other Elizabethkingia species and strains, and exhibited open pan-genome characteristics, with two strains being nearly identical and the third divergent. These strains were susceptible only to trimethoprim/sulfamethoxazole and ciprofloxacin amongst 16 antibiotics in minimum inhibitory tests. The resistome exhibited a high diversity of resistance genes, including 5 different lactamase- and 18 efflux protein- encoding genes. Forty-four genes encoding virulence factors were conserved among the strains. Sialic acid transporters and curli synthesis genes were well conserved in E. meningoseptica but absent in E. anophelis and E. miricola. E. meningoseptica carried several genes contributing to biofilm formation. 58 glycoside hydrolases (GH) and 25 putative polysaccharide utilization loci (PULs) were found. The strains carried numerous genes encoding two-component system proteins (56), transcription factor proteins (187~191), and DNA-binding proteins (6~7). Several prophages and CRISPR/Cas elements were uniquely present in the genomes.

Klíčová slova:

Antibiotic resistance – Antibiotics – Antimicrobial resistance – Comparative genomics – DNA-binding proteins – Genome analysis – Sialic acids – Virulence factors

Zdroje

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Comparative genomic analyses reveal diverse virulence factors and antimicrobial resistance mechanisms in clinical Elizabethkingia meningoseptica strains

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