Artificial fingerprints for cross-comparison of forensic DNA and protein recovery methods
Autoři:
Danielle S. LeSassier aff001; Kathleen Q. Schulte aff001; Tara E. Manley aff001; Alan R. Smith aff001; Megan L. Powals aff001; Nicolette C. Albright aff001; Benjamin C. Ludolph aff001; Katharina L. Weber aff001; August E. Woerner aff002; Myles W. Gardner aff001; F. Curtis Hewitt aff001
Působiště autorů:
Signature Science, LLC, Austin, Texas, United States of America
aff001; Center for Human Identification, University of North Texas Health Science Center, Fort Worth, Texas, United States of America
aff002; Graduate School of Biomedical Sciences, University of North Texas Health Science Center, Fort Worth, Texas, United States of America
aff003
Vyšlo v časopise:
PLoS ONE 14(10)
Kategorie:
Research Article
doi:
https://doi.org/10.1371/journal.pone.0223170
Souhrn
Quantitative genomic and proteomic evaluation of human latent fingerprint depositions represents a challenge within the forensic field, due to the high variability in the amount of DNA and protein initially deposited. To better assess recovery techniques for touch depositions, we present a method to produce simple and customizable artificial fingerprints. These artificial fingerprint samples include the primary components of a typical latent fingerprint, specifically sebaceous fluid, eccrine perspiration, extracellular DNA, and proteinaceous epidermal skin material (i.e., shed skin cells). A commercially available emulsion of sebaceous and eccrine perspiration material provides a chemically-relevant suspension solution for fingerprint deposition, simplifying artificial fingerprint production. Extracted human genomic DNA is added to accurately mimic the extracellular DNA content of a typical latent print and comparable DNA yields are recovered from the artificial prints relative to human prints across surface types. Capitalizing on recent advancements in the use of protein sequence identification for human forensic analysis, these samples also contain a representative quantity of protein, originating from epidermal skin cells collected from the fingers and palms of volunteers. Proteomic sequencing by liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis indicates a high level of protein overlap between artificial and latent prints. Data are available via ProteomeXchange with identifier PXD015445. By including known quantities of DNA and protein into each artificial print, this method enables total DNA and protein recovery to be quantitatively assessed across different sample collection and extraction methods to better evaluate extraction efficiency. Collectively, these artificial fingerprint samples are simple to make, highly versatile and customizable, and accurately represent the biochemical composition and biological signatures of human fingerprints.
Klíčová slova:
Cytoskeleton – DNA extraction – Genetic fingerprinting – Glass – Chemical deposition – Keratins – Forensics – Dactyloscopy
Zdroje
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PLOS One
2019 Číslo 10
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