Method development and validation for rapid identification of epigallocatechin gallate using ultra-high performance liquid chromatography
Autoři:
Ramakrishna U. V. aff001; Shyam Sunder R. aff002; Rajesh Kumar K. aff001; Sukesh Narayan Sinha aff001
Působiště autorů:
Food Safety Division, ICMR—National Institute of Nutrition, Tarnaka, Hyderabad, Telangana, India
aff001; University College of Technology, Osmania University, Tarnaka, Hyderabad, Telangana, India
aff002
Vyšlo v časopise:
PLoS ONE 15(1)
Kategorie:
Research Article
doi:
https://doi.org/10.1371/journal.pone.0227569
Souhrn
Although Epigallocatechin gallate (EGCG) is the most available and beneficial catechin found in tea, its auto-oxidation property may lead to toxicity when consumed in large quantities. Thus, there is a need to quantify the EGCG, which enables to study the pharmacological characteristics of the compound. The study aimed to develop and validate a rapid and accurate analytical method for quantitative determination of EGCG. Standard EGCG was used to conduct trials for the optimization of the analytical method using Ultra–High Performance Liquid Chromatography (UHPLC). Tests for validation (specificity, linearity, accuracy, system suitability, method precision, robustness, and ruggedness) were performed. The preliminary trials yielded an analytical method with good peak shape and acceptable system suitability which was further validated. The method was shown to be specific, with a linear correlation coefficient of > 0.9996 and accurate with acceptable recovery rate (99.1% to 100.4%). Acceptable system suitability and method precision were confirmed with a relative standard deviation (less than 2%). Further, robustness and ruggedness experiments also demonstrated the suitability of the present analytical method. The method developed for determination of EGCG was validated as per the International Council for Harmonisation of Technical Requirements for Pharmaceuticals for Human Use (ICH) guidelines and thus can be used in routine compliance tests in the laboratory for further studying/characterizing the properties of EGCG.
Klíčová slova:
Flow rate – High performance liquid chromatography – Chromatographic techniques – Phase determination – Reversed-phase high performance liquid chromatography – Signal filtering – Tea – Toxicity
Zdroje
1. Dou Q.P, Tea in Health and Disease. Nutrients. 2019; 11: 929–931.
2. Khan N, Mukhtar H, Tea Polyphenols in Promotion of Human Health. Nutrients. 2019; 11: 39–55.
3. Yang C.S, Wang H, Mechanistic issues concerning cancer prevention by tea catechins. Mol. Nutr. Food Res. 2011; 55: 819–831. doi: 10.1002/mnfr.201100036 21538856
4. Ramakrishna U. V, Shyam P, Sinha S. N, Vakdevi V, Drug-Repositioning of the Gallate group from EGCG for the Repurposing. Eur J Pharm Med Res. 2016; 3: 253–262.
5. Shen G, Xu C, Hu R, Jain M.R, Nair S, Lin W, et al., Comparison of (-)-epigallocatechin-3-gallate elicited liver and small intestine gene expression profiles between C57BL/6J mice and C57BL/6J/Nrf2 (-/-) mice. Pharm. Res. 2005; 22: 1805–1820. doi: 10.1007/s11095-005-7546-8 16132347
6. Mazzanti G, Di S.A, Vitalone A, Hepatotoxicity of green tea: An update. Arch. Toxicol. 2015; 89: 1175–1191. doi: 10.1007/s00204-015-1521-x 25975988
7. He Q, Yao K, Jia D, Fan H, Liao X, Shi B, Determination of total catechins in tea extracts by HPLC and spectrophotometry. Nat Prod Res. 2009; 23: 93–100. doi: 10.1080/14786410801886682 19140075
8. Hirun S, Roach P.D, An improved solvent extraction method for the analysis of catechins and caffeine in green tea. J Food Nutr Res. 2011; 50: 160–166.
9. Fernando C.D, Soysa P, Simple isocratic method for simultaneous determination of caffeine and catechins in tea products by HPLC. Springerplus. 2016; 5: 970–974. doi: 10.1186/s40064-016-2672-9 27429880
10. Lee LS, Kim S.H., Kim Y.B, Kim Y.C, Quantitative Analysis of Major Constituents in Green Tea with Different Plucking Periods and Their Antioxidant Activity. Molecules. 2014; 19: 9173–9186; doi: 10.3390/molecules19079173 24988187
11. Avadhani K.S, Reddy A.M, Udupa N, Mutalik S, Development and validation of RP-HPLC method for estimation of epigallocatechin -3-gallate (EGCG) in lipid based nanoformulations. RJPT. 2016; 9: 725–730
12. Pan HB, Zhang D, Li B, Wu Y.Y, Tu Y.Y, A Rapid UPLC Method for Simultaneous Analysis of Caffeine and 13 Index Polyphenols in Black Tea. J Chromatogr Sci. 2017; 55: 495–496. doi: 10.1093/chromsci/bmw197 28203771
13. El-Kayal M.O, Sayed M.N, Mortada N.D, Elkheshen S, Development and validation of a simple and rapid UPLC method for the in-vitro estimation of (-)-epigallocatechin-3-gallate in lipid-based formulations. Eur J Med Chem. 2018; 9: 7–12
14. Park J.E, Kim T.E, Shin K.H, Quantitative Analysis of Four Catechins from Green Tea Extract in Human Plasma Using Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry for Pharmacokinetic Studies. Molecules. 2018; 23: 984–1000.
15. Zhao Y, Chen P, Lin L, Harnly J.M, Yu (Lucy) L, Li Z, Tentative identification, quantitation, and principal component analysis of green Pu-erh, green, and white teas using UPLC/DAD/MS. Food Chem. 2011; 126: 1269–1277. doi: 10.1016/j.foodchem.2010.11.055 25544798
16. Pongsuwan W, Bamba T, Harada K, Yonetani T, Kobayashi A, Fukusaki E, High-Throughput Technique for Comprehensive Analysis of Japanese Green Tea Quality Assessment Using Ultra-Performance Liquid Chromatography with Time-of-Flight Mass Spectrometry (UPLC/TOF MS). J. Agric. Food Chem. 2008; 56: 10705–10708 doi: 10.1021/jf8018003 18973299
17. ICH Topic Q 2 (R1) Validation of Analytical Procedures: Text and Methodology, European Medicines Agency, 1995, CPMP/ICH/381/95
18. Ravisankar P, Navya C.N, Pravallika D. and Navya Sri D, A Review on Step-by-Step Analytical Method Validation, IOSR J Pharm. 2015; 5: 7–19.
Článek vyšel v časopise
PLOS One
2020 Číslo 1
- S diagnostikou Parkinsonovy nemoci může nově pomoci AI nástroj pro hodnocení mrkacího reflexu
- Proč při poslechu některé muziky prostě musíme tančit?
- Je libo čepici místo mozkového implantátu?
- Chůze do schodů pomáhá prodloužit život a vyhnout se srdečním chorobám
- Pomůže v budoucnu s triáží na pohotovostech umělá inteligence?
Nejčtenější v tomto čísle
- Severity of misophonia symptoms is associated with worse cognitive control when exposed to misophonia trigger sounds
- Chemical analysis of snus products from the United States and northern Europe
- Calcium dobesilate reduces VEGF signaling by interfering with heparan sulfate binding site and protects from vascular complications in diabetic mice
- Effect of Lactobacillus acidophilus D2/CSL (CECT 4529) supplementation in drinking water on chicken crop and caeca microbiome
Zvyšte si kvalifikaci online z pohodlí domova
Všechny kurzy