An integrated vitamin E-coated polymer hybrid nanoplatform: A lucrative option for an enhanced in vitro macrophage retention for an anti-hepatitis B therapeutic prospect
Autoři:
Mohamed Hamdi aff001; Hend Mohamed Abdel-Bar aff001; Enas Elmowafy aff002; Khuloud T. Al-Jamal aff003; Gehanne A. S. Awad aff002
Působiště autorů:
Department of Pharmaceutics, Faculty of Pharmacy, University of Sadat City, Sadat City, Egypt
aff001; Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Ain Shams University, Sadat City, Egypt
aff002; Institute of Pharmaceutical Science, Faculty of Life Sciences & Medicine, King’s College London, England, United Kingdom
aff003
Vyšlo v časopise:
PLoS ONE 15(1)
Kategorie:
Research Article
doi:
https://doi.org/10.1371/journal.pone.0227231
Souhrn
A platform capable of specifically delivering an antiviral drug to the liver infected with hepatitis B is a major concern in hepatology. Vaccination has had a major effect on decreasing the emerging numbers of new cases of infection. However, the total elimination of the hepatitis B virus from the body requires prolonged therapy. In this work, we aimed to target the liver macrophages with lipid polymer hybrid nanoparticles (LPH), combining the merit of polymeric nanoparticles and lipid vesicles. The hydrophilic antiviral drug, entecavir (E), loaded LPH nanoparticles were optimized and physicochemically characterized. A modulated lipidic corona, as well as, an additional coat with vitamin E were used to extend the drug release enhance the macrophage uptake. The selected vitamin E coated LPH nanoparticles enriched with lecithin-glyceryl monostearate lipid shell exhibited high entrapment for E (80.47%), a size ≤ 200 nm for liver passive targeting, extended release over one week, proven serum stability, retained stability after refrigeration storage for 6 months. Upon macrophage uptake in vitro assessment, the presented formulation displayed promising traits, enhancing the cellular retention in J774 macrophages cells. In vivo and antiviral activity futuristic studies would help in the potential application of the ELPH in hepatitis B control.
Klíčová slova:
Atomic force microscopy – Confocal laser microscopy – Lipids – Macrophages – Nanoparticles – Polymers – Vitamin E – Nanocarriers
Zdroje
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