Discovery of Jogalong virus, a novel hepacivirus identified in a Culex annulirostris (Skuse) mosquito from the Kimberley region of Western Australia
Autoři:
Simon H. Williams aff001; Avram Levy aff003; Rachel A. Yates aff001; Nilusha Somaweera aff004; Peter J. Neville aff004; Jay Nicholson aff004; Michael D. A. Lindsay aff004; John S. Mackenzie aff003; Komal Jain aff001; Allison Imrie aff002; David W. Smith aff002; W. Ian Lipkin aff001
Působiště autorů:
Center for Infection and Immunity, Mailman School of Public Health of Columbia University, New York, New York, United States of America
aff001; Faculty of Health and Medical Sciences, University of Western Australia, Nedlands, Western Australia, Australia
aff002; PathWest Laboratory Medicine WA, Nedlands, Western Australia, Australia
aff003; Environmental Health Directorate, Public and Aboriginal Health Division, Department of Health, Western Australia, Perth, Western Australia, Australia
aff004; Faculty of Health Sciences, Curtin University, Perth, Western Australia, Australia
aff005
Vyšlo v časopise:
PLoS ONE 15(1)
Kategorie:
Research Article
doi:
https://doi.org/10.1371/journal.pone.0227114
Souhrn
The discovery of hepaciviruses in non-human hosts has accelerated following the advancement of high-throughput sequencing technology. Hepaciviruses have now been described in reptiles, fish, birds, and an extensive array of mammals. Using metagenomic sequencing on pooled samples of field-collected Culex annulirostris mosquitoes, we discovered a divergent hepacivirus-like sequence, named Jogalong virus, from the Kimberley region in northern Western Australia. Using PCR, we screened the same 300 individual mosquitoes and found just a single positive sample (1/300, 0.33%). Phylogenetic analysis of the hepacivirus NS5B protein places Jogalong virus within the genus Hepacivirus but on a distinct and deeply rooted monophyletic branch shared with duck hepacivirus, suggesting a notably different evolutionary history. Vertebrate barcoding PCR targeting two mitochondrial genes, cytochrome c oxidase subunit I and cytochrome b, indicated that the Jogalong virus-positive mosquito had recently fed on the tawny frogmouth (Podargus strigoides), although it is currently unknown whether this bird species contributes to the natural ecology of this virus.
Klíčová slova:
Birds – Blood – Ducks – Hepacivirus – Mosquitoes – Phylogenetic analysis – Polymerase chain reaction – Sequence assembly tools
Zdroje
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