Enzyme response of activated sludge to a mixture of emerging contaminants in continuous exposure
Autoři:
Georgiana Amariei aff001; Karina Boltes aff001; Roberto Rosal aff001; Pedro Leton aff001
Působiště autorů:
Department of Chemical Engineering, University of Alcalá, Alcalá de Henares, Madrid, Spain
aff001
Vyšlo v časopise:
PLoS ONE 15(1)
Kategorie:
Research Article
doi:
https://doi.org/10.1371/journal.pone.0227267
Souhrn
The relevant information about the impacts caused by presence of emerging pollutants in mixtures on the ecological environment, especially on the more vulnerable compartments such as activated sludge (AS) is relatively limited. This study investigated the effect of ibuprofen (IBU) and triclosan (TCS), alone and in combination to the performance and enzymatic activity of AS bacterial community. The assays were carried out in a pilot AS reactor operating for two-weeks under continuous dosage of pollutants. The microbial activity was tracked by measuring oxygen uptake rate, esterase activity, oxidative stress and antioxidant enzyme activities. It was found that IBU and TCS had no acute toxic effects on reactor biomass concentration. TCS led to significant decrease of COD removal efficiency, which dropped from 90% to 35%. Continuous exposure to IBU, TCS and their mixtures increased the activities of glutathione s-transferase (GST) and esterase as a response to oxidative damage. A high increase in GST activity was associated with non-reversible toxic damage while peaks of esterase activity combined with moderate GST increase were attributed to an adaptive response.
Klíčová slova:
Bacteria – Esterases – Glutathione chromatography – Chemical oxygen demand – Oxidative stress – Pollutants – Sludge – Water pollution
Zdroje
1. Boxall AB, Rudd MA, Brooks BW, Caldwell DJ, Choi K, Hickmann S, et al. Pharmaceuticals and personal care products in the environment: what are the big questions? Environmental Health Perspectives. 2012;120(9):1221–9. doi: 10.1289/ehp.1104477 22647657
2. Wilkinson JL, Hooda PS, Barker J, Barton S, Swinden J. Ecotoxic pharmaceuticals, personal care products, and other emerging contaminants: A review of environmental, receptor-mediated, developmental, and epigenetic toxicity with discussion of proposed toxicity to humans. Critical Reviews in Environmental Science and Technology. 2016;46(4):336–81.
3. Ebele AJ, Abdallah MAE, Harrad S. Pharmaceuticals and personal care products (PPCPs) in the freshwater aquatic environment. Emerging Contaminants. 2017;3(1):1–16.
4. McNamara PJ, LaPara TM, Novak PJ. The impacts of triclosan on anaerobic community structures, function, and antimicrobial resistance. Environmental science & technology. 2014;48(13):7393–400.
5. Borgman O, Chefetz B. Combined effects of biosolids application and irrigation with reclaimed wastewater on transport of pharmaceutical compounds in arable soils. Water research. 2013;47(10):3431–43. doi: 10.1016/j.watres.2013.03.045 23591105
6. Paiga P, Correia M, Fernandes MJ, Silva A, Carvalho M, Vieira J, et al. Assessment of 83 pharmaceuticals in WWTP influent and effluent samples by UHPLC-MS/MS: Hourly variation. The Science of the total environment. 2019;648:582–600. doi: 10.1016/j.scitotenv.2018.08.129 30121536
7. Collado N, Rodriguez-Mozaz S, Gros M, Rubirola A, Barcelo D, Comas J, et al. Pharmaceuticals occurrence in a WWTP with significant industrial contribution and its input into the river system. Environmental pollution. 2014;185:202–12. doi: 10.1016/j.envpol.2013.10.040 24286695
8. Palli L, Spina F, Varese GC, Vincenzi M, Aragno M, Arcangeli G, et al. Occurrence of selected pharmaceuticals in wastewater treatment plants of Tuscany: An effect-based approach to evaluate the potential environmental impact. International journal of hygiene and environmental health. 2019;222(4):717–25. doi: 10.1016/j.ijheh.2019.05.006 31101503
9. Ashfaq M, Li Y, Rehman MSU, Zubair M, Mustafa G, Nazar MF, et al. Occurrence, spatial variation and risk assessment of pharmaceuticals and personal care products in urban wastewater, canal surface water, and their sediments: A case study of Lahore, Pakistan. The Science of the total environment. 2019;688:653–63. doi: 10.1016/j.scitotenv.2019.06.285 31254831
10. Thiebault T, Boussafir M, Le Milbeau C. Occurrence and removal efficiency of pharmaceuticals in an urban wastewater treatment plant: Mass balance, fate and consumption assessment. Journal of Environmental Chemical Engineering. 2017;5(3):2894–902.
11. Grandclement C, Seyssiecq I, Piram A, Wong-Wah-Chung P, Vanot G, Tiliacos N, et al. From the conventional biological wastewater treatment to hybrid processes, the evaluation of organic micropollutant removal: A review. Water research. 2017;111:297–317. doi: 10.1016/j.watres.2017.01.005 28104517
12. Liu JL, Wong MH. Pharmaceuticals and personal care products (PPCPs): a review on environmental contamination in China. Environment international. 2013;59:208–24. doi: 10.1016/j.envint.2013.06.012 23838081
13. Alvarino T, Katsou E, Malamis S, Suarez S, Omil F, Fatone F. Inhibition of biomass activity in the via nitrite nitrogen removal processes by veterinary pharmaceuticals. Bioresource technology. 2014;152:477–83. doi: 10.1016/j.biortech.2013.10.107 24333624
14. Ren S. Assessing wastewater toxicity to activated sludge: recent research and developments. Environment International. 2004;30(8):1151–64. doi: 10.1016/j.envint.2004.06.003 15337358
15. Amorim CL, Maia AS, Mesquita RB, Rangel AO, van Loosdrecht MC, Tiritan ME, et al. Performance of aerobic granular sludge in a sequencing batch bioreactor exposed to ofloxacin, norfloxacin and ciprofloxacin. Water research. 2014;50:101–13. doi: 10.1016/j.watres.2013.10.043 24361707
16. Jiang C, Geng J, Hu H, Ma H, Gao X, Ren H. Impact of selected non-steroidal anti-inflammatory pharmaceuticals on microbial community assembly and activity in sequencing batch reactors. PLOS ONE. 2017;12(6):e0179236. doi: 10.1371/journal.pone.0179236 28640897
17. Sui Q, Huang J, Deng S, Chen W, Yu G. Seasonal variation in the occurrence and removal of pharmaceuticals and personal care products in different biological wastewater treatment processes. Environmental science & technology. 2011;45(8):3341–8.
18. Zhang Y, Geng J, Ma H, Ren H, Xu K, Ding L. Characterization of microbial community and antibiotic resistance genes in activated sludge under tetracycline and sulfamethoxazole selection pressure. Science of The Total Environment. 2016;571:479–86. doi: 10.1016/j.scitotenv.2016.07.014 27395074
19. Arriaga S, de Jonge N, Nielsen ML, Andersen HR, Borregaard V, Jewel K, et al. Evaluation of a membrane bioreactor system as post-treatment in waste water treatment for better removal of micropollutants. Water research. 2016;107:37–46. doi: 10.1016/j.watres.2016.10.046 27794216
20. Nguyen LN, Nghiem LD, Pramanik BK, Oh S. Cometabolic biotransformation and impacts of the anti-inflammatory drug diclofenac on activated sludge microbial communities. The Science of the total environment. 2019;657:739–45. doi: 10.1016/j.scitotenv.2018.12.094 30677939
21. Yu X, Nishimura F, Hidaka T. Impact of Long-Term Perfluorooctanoic Acid (PFOA) Exposure on Activated Sludge Process. Water, Air, & Soil Pollution. 2018;229(4).
22. Zhang W, Huang MH, Qi FF, Sun PZ, Van Ginkel SW. Effect of trace tetracycline concentrations on the structure of a microbial community and the development of tetracycline resistance genes in sequencing batch reactors. Bioresource technology. 2013;150:9–14. doi: 10.1016/j.biortech.2013.09.081 24140945
23. Kraigher B, Kosjek T, Heath E, Kompare B, Mandic-Mulec I. Influence of pharmaceutical residues on the structure of activated sludge bacterial communities in wastewater treatment bioreactors. Water research. 2008;42(17):4578–88. doi: 10.1016/j.watres.2008.08.006 18786690
24. Collado N, B G, Marti E., Ferrando-Climent L., Rodriguez-Mozaz S., Barceló D., Comasa J. R-R I. Effects on activated sludge bacterial community exposed to sulfamethoxazole. Chemosphere. 2013.
25. Yazdanbakhsh AR, Rafiee M, Daraei H, Amoozegar MA. Responses of flocculated activated sludge to bimetallic Ag-Fe nanoparticles toxicity: Performance, activity enzymatic, and bacterial community shift. Journal of hazardous materials. 2019;366:114–23. doi: 10.1016/j.jhazmat.2018.11.098 30504079
26. Nakada N, Tanishima T, Shinohara H, Kiri K, Takada H. Pharmaceutical chemicals and endocrine disrupters in municipal wastewater in Tokyo and their removal during activated sludge treatment. Water research. 2006;40(17):3297–303. doi: 10.1016/j.watres.2006.06.039 16938339
27. Rosal R, Rodríguez A, Perdigón-Melón JA, Petre A, García-Calvo E, Gómez MJ, et al. Occurrence of emerging pollutants in urban wastewater and their removal through biological treatment followed by ozonation. Water research. 2010;44(2):578–88. doi: 10.1016/j.watres.2009.07.004 19628245
28. Martin J, Camacho-Munoz D, Santos JL, Aparicio I, Alonso E. Occurrence of pharmaceutical compounds in wastewater and sludge from wastewater treatment plants: removal and ecotoxicological impact of wastewater discharges and sludge disposal. Journal of hazardous materials. 2012;239–240:40–7. doi: 10.1016/j.jhazmat.2012.04.068 22608399
29. Bedoux G, Roig B, Thomas O, Dupont V, Le Bot B. Occurrence and toxicity of antimicrobial triclosan and by-products in the environment. Environmental science and pollution research international. 2012;19(4):1044–65. doi: 10.1007/s11356-011-0632-z 22057832
30. Drury B, Scott J, Rosi-Marshall EJ, Kelly JJ. Triclosan exposure increases triclosan resistance and influences taxonomic composition of benthic bacterial communities. Environ Sci Technol. 2013;47(15):8923–30. doi: 10.1021/es401919k 23865377
31. Amariei G, Boltes K, Rosal R, Leton P. Toxicological interactions of ibuprofen and triclosan on biological activity of activated sludge. Journal of hazardous materials. 2017;334:193–200. doi: 10.1016/j.jhazmat.2017.04.018 28412629
32. OECD. Test No. 209: Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation). OECD Guidelines for the Testing of Chemicals, Section 2, No 310. Paris: OECD Publishing; 2010.
33. Behera SK, Oh SY, Park HS. Sorption of triclosan onto activated carbon, kaolinite and montmorillonite: Effects of pH, ionic strength, and humic acid. Journal of hazardous materials. 2010;179(1):684–91.
34. Petrie B, McAdam EJ, Lester JN, Cartmell E. Assessing potential modifications to the activated sludge process to improve simultaneous removal of a diverse range of micropollutants. Water research. 2014;62:180–92. doi: 10.1016/j.watres.2014.05.036 24956600
35. BREEUWER P DROCOURT J-L, BUNSCHOTEN N, ZWIETERING MH, ROMBOUTS FM, ABEE T. Characterization of Uptake and Hydrolysis of Fluorescein Diacetate and Carboxyfluorescein Diacetate by Intracellular Esterases in Saccharomyces cerevisiae, Which Result in Accumulation of Fluorescent Product. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1995;61(4):6.
36. Gomes A, Fernandes E, Lima JL. Fluorescence probes used for detection of reactive oxygen species. Journal of biochemical and biophysical methods. 2005;65(2–3):45–80. doi: 10.1016/j.jbbm.2005.10.003 16297980
37. Krah D, Ghattas AK, Wick A, Broder K, Ternes TA. Micropollutant degradation via extracted native enzymes from activated sludge. Water research. 2016;95:348–60. doi: 10.1016/j.watres.2016.03.037 27017196
38. Nauen R, Stumpf N. Fluorometric microplate assay to measure glutathione S-transferase activity in insects and mites using monochlorobimane. Analytical Biochemistry. 2002;303(2):194–8. doi: 10.1006/abio.2002.5578 11950219
39. Nash T. The colorimetric estimation of formaldehyde by means of the Hantzsch reaction. Biochemical Journal. 1953;55(3):416–21. doi: 10.1042/bj0550416 13105648
40. Gros M, Petrović M, Barceló D. Multi-residue analytical methods using LC-tandem MS for the determination of pharmaceuticals in environmental and wastewater samples: A review. Analytical and Bioanalytical Chemistry. 2006;386(4):941–52. doi: 10.1007/s00216-006-0586-z 16830112
41. Dhillon GS, Kaur S, Pulicharla R, Brar SK, Cledón M, Verma M, et al. Triclosan: Current status, occurrence, environmental risks and bioaccumulation potential. International Journal of Environmental Research and Public Health. 2015;12(5):5657–84. doi: 10.3390/ijerph120505657 26006133
42. Liu X, Yin H, Tang S, Feng M, Peng H, Lu G, et al. Effects of single and combined copper/perfluorooctane sulfonate on sequencing batch reactor process and microbial community in activated sludge. Bioresource technology. 2017;238:407–15. doi: 10.1016/j.biortech.2017.04.045 28458174
43. Stasinakis AS, Mamais D, Thomaidis NS, Danika E, Gatidou G, Lekkas TD. Inhibitory effect of triclosan and nonylphenol on respiration rates and ammonia removal in activated sludge systems. Ecotoxicology and Environmental Safety. 2008;70(2):199–206. doi: 10.1016/j.ecoenv.2007.12.011 18237779
44. He H, Chen Y, Li X, Cheng Y, Yang C, Zeng G. Influence of salinity on microorganisms in activated sludge processes: A review. International Biodeterioration & Biodegradation. 2017;119:520–7.
45. Patel M, Kumar R, Kishor K, Mlsna T, Pittman CU Jr., Mohan D. Pharmaceuticals of Emerging Concern in Aquatic Systems: Chemistry, Occurrence, Effects, and Removal Methods. Chemical reviews. 2019;119(6):3510–673. doi: 10.1021/acs.chemrev.8b00299 30830758
46. Suarez S, Lema JM, Omil F. Removal of Pharmaceutical and Personal Care Products (PPCPs) under nitrifying and denitrifying conditions. Water research. 2010;44(10):3214–24. doi: 10.1016/j.watres.2010.02.040 20338614
47. Li ZH, Ma ZB, Yu HQ. Respiration adaptation of activated sludge under dissolved oxygen and hypochlorite stressed conditions. Bioresource technology. 2018;248:171–8. doi: 10.1016/j.biortech.2017.06.166 28736142
48. Boczar BA, Forney LJ, Begley WM, Larson RJ, Federle TW. Characterization and distribution of esterase activity in activated sludge. Water research. 2001;35(17):4208–16. doi: 10.1016/s0043-1354(01)00150-6 11791851
49. Klatt CG, LaPara TM. Aerobic biological treatment of synthetic municipal wastewater in membrane-coupled bioreactors. Biotechnology and bioengineering. 2003;82(3):313–20. doi: 10.1002/bit.10572 12599258
50. Martínez-Martínez M, Lores I, Peña-García C, Bargiela R, Reyes-Duarte D, Guazzaroni ME, et al. Biochemical studies on a versatile esterase that is most catalytically active with polyaromatic esters. Microbial Biotechnology. 2014;7(2):184–91. doi: 10.1111/1751-7915.12107 24418210
51. Reuter S, Gupta SC, Chaturvedi MM, Aggarwal BB. Oxidative stress, inflammation, and cancer: How are they linked? Free Radical Biology & Medicine. 2010;49(11):1603–16.
52. Cavanagh JE, Trought K, Mitchell C, Northcott G, Tremblay LA. Assessment of endocrine disruption and oxidative potential of bisphenol-A, triclosan, nonylphenol, diethylhexyl phthalate, galaxolide, and carbamazepine, common contaminants of municipal biosolids. Toxicology In Vitro. 2018;48:342–9. doi: 10.1016/j.tiv.2018.02.003 29427707
53. Zhang Y, Meng D, Wang Z, Guo H, Wang Y. Oxidative stress response in two representative bacteria exposed to atrazine. FEMS microbiology letters. 2012;334(2):95–101. doi: 10.1111/j.1574-6968.2012.02625.x 22724442
54. Geret F, Serafim A, Barreira L, Bebianno MJ. Effect of cadmium on antioxidant enzyme activities and lipid peroxidation in the gills of the clam Ruditapes decussatus. Biomarkers. 2002;7(3):242–56. doi: 10.1080/13547500210125040 12141067
55. Lü Z, Sang L, Li Z, Min H. Catalase and superoxide dismutase activities in a Stenotrophomonas maltophilia WZ2 resistant to herbicide pollution. Ecotoxicology and Environmental Safety. 2009;72(1):136–43. doi: 10.1016/j.ecoenv.2008.01.009 18304632
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