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Estimating relative CWD susceptibility and disease progression in farmed white-tailed deer with rare PRNP alleles


Autoři: Nicholas J. Haley aff001;  Kahla Merrett aff001;  Amy Buros Stein aff002;  Dennis Simpson aff003;  Andrew Carlson aff003;  Gordon Mitchell aff004;  Antanas Staskevicius aff004;  Tracy Nichols aff005;  Aaron D. Lehmkuhl aff006;  Bruce V. Thomsen aff006
Působiště autorů: Department of Microbiology and Immunology, College of Graduate Studies, Midwestern University, Glendale, Arizona aff001;  Office of Research and Sponsored Programs, Midwestern University, Glendale, Arizona aff002;  Simpson Whitetails Genetic Testing, Belleville, Michigan aff003;  National and OIE Reference Laboratory for Scrapie and CWD, Canadian Food Inspection Agency, Ottawa Laboratory-Fallowfield, Ottawa, Ontario, Canada aff004;  United States Department of Agriculture, APHIS, Veterinary Services, Cervid Health Program, Fort Collins, Colorado, United States of America aff005;  United States Department of Agriculture, APHIS, Veterinary Services, National Veterinary Services Laboratories, Ames, Iowa, United States of America aff006;  United States Department of Agriculture, APHIS, Veterinary Services, Center for Veterinary Biologics, Ames, Iowa, United States of America aff007
Vyšlo v časopise: PLoS ONE 14(12)
Kategorie: Research Article
doi: https://doi.org/10.1371/journal.pone.0224342

Souhrn

Chronic wasting disease is a prion disease affecting both free-ranging and farmed cervids in North America and Scandinavia. A range of cervid species have been found to be susceptible, each with variations in the gene for the normal prion protein, PRNP, reportedly influencing both disease susceptibility and progression in the respective hosts. Despite the finding of several different PRNP alleles in white-tailed deer, the majority of past research has focused on two of the more common alleles identified—the 96G and 96S alleles. In the present study, we evaluate both infection status and disease stage in nearly 2100 farmed deer depopulated in the United States and Canada, including 714 CWD-positive deer and correlate our findings with PRNP genotype, including the more rare 95H, 116G, and 226K alleles. We found significant differences in either likelihood of being found infected or disease stage (and in many cases both) at the time of depopulation in all genotypes present, relative to the most common 96GG genotype. Despite high prevalence in many of the herds examined, infection was not found in several of the reported genotypes. These findings suggest that additional research is necessary to more properly define the role that these genotypes may play in managing CWD in both farmed and free-ranging white-tailed deer, with consideration for factors including relative fitness levels, incubation periods, and the kinetics of shedding in animals with these rare genotypes.

Klíčová slova:

Alleles – Animal prion diseases – Canada – Deer – Chronic wasting disease – United States – Variant genotypes – Veterinary diseases


Zdroje

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