Interferometric fluorescence cross correlation spectroscopy
Autoři:
Ipsita Saha aff001; Saveez Saffarian aff001
Působiště autorů:
Center for Cell and Genome Science, University of Utah, Salt Lake City, Utah, United States of America
aff001; Department of Physics and Astronomy, University of Utah, Salt Lake City, Utah, United States of America
aff002; Department of Biology, University of Utah, Salt Lake City, Utah, United States of America
aff003
Vyšlo v časopise:
PLoS ONE 14(12)
Kategorie:
Research Article
doi:
https://doi.org/10.1371/journal.pone.0225797
Souhrn
Measuring transport properties like diffusion and directional flow is essential for understanding dynamics within heterogeneous systems including living cells and novel materials. Fluorescent molecules traveling within these inhomogeneous environments under the forces of Brownian motion and flow exhibit fluctuations in their concentration, which are directly linked to the transport properties. We present a method utilizing single photon interference and fluorescence correlation spectroscopy (FCS) to simultaneously measure transport of fluorescent molecules within aqueous samples. Our method, within seconds, measures transport in thousands of homogenous voxels (100 nm)3 and under certain conditions, eliminates photo-physical artifacts associated with blinking of fluorescent molecules. A comprehensive theoretical framework is presented and validated by measuring transport of quantum dots, associated with VSV-G receptor along cellular membranes as well as within viscous gels.
Klíčová slova:
Cameras – Cell membranes – Fluorescence – Mass diffusivity – Wave interference – Quantum dots – Fluorescence spectroscopy – Quantum interference
Zdroje
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