Domestication may affect the maternal mRNA profile in unfertilized eggs, potentially impacting the embryonic development of Eurasian perch (Perca fluviatilis)

English version

Autoři: Tainá Rocha de Almeida ^aff001; Maud Alix ^aff001; Aurélie Le Cam ^aff002; Christophe Klopp ^aff003; Jérôme Montfort ^aff002; Lola Toomey ^aff001; Yannick Ledoré ^aff001; Julien Bobe ^aff002; Dominique Chardard ^aff001; Bérénice Schaerlinger ^aff001; Pascal Fontaine ^aff001
Působiště autorů: UR AFPA, University of Lorraine, INRA, Nancy, France ^aff001; LPGP, UR1037 Fish Physiology and Genomics, INRA, Rennes, France ^aff002; Sigenae, MIAT, INRA, Castanet-Tolosan, Toulouse, France ^aff003
Vyšlo v časopise: PLoS ONE 14(12)
Kategorie: Research Article
doi: https://doi.org/10.1371/journal.pone.0226878

Souhrn

Domestication is an evolutionary process during which we expect populations to progressively adapt to an environment controlled by humans. It is accompanied by genetic and presumably epigenetic changes potentially leading to modifications in the transcriptomic profile in various tissues. Reproduction is a key function often affected by this process in numerous species, regardless of the mechanism. The maternal mRNA in fish eggs is crucial for the proper embryogenesis. Our working hypothesis is that modifications of maternal mRNAs may reflect potential genetic and/or epigenetic modifications occurring during domestication and could have consequences during embryogenesis. Consequently, we investigated the trancriptomic profile of unfertilized eggs from two populations of Eurasian perch. These two populations differed by their domestication histories (F1 vs. F7+–at least seven generations of reproduction in captivity) and were genetically differentiated (F_ST = 0.1055, p<0.05). A broad follow up of the oogenesis progression failed to show significant differences during oogenesis between populations. However, the F1 population spawned earlier with embryos presenting an overall higher survivorship than those from the F7+ population. The transcriptomic profile of unfertilized eggs showed 358 differentially expressed genes between populations. In conclusion, our data suggests that the domestication process may influence the regulation of the maternal transcripts in fish eggs, which could in turn explain differences of developmental success.

Klíčová slova:

Domestic animals – Embryos – Fish farming – Gene expression – Microarrays – Spawning – Transcriptome analysis – Oogenesis

Zdroje

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Domestication may affect the maternal mRNA profile in unfertilized eggs, potentially impacting the embryonic development of Eurasian perch (Perca fluviatilis)

Souhrn

Klíčová slova:

Zdroje

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