Cell sources of inflammatory mediators present in bone marrow areas inside the meniscus
Autoři:
Francisco Airton Castro Rocha aff001; Virgínia Claudia Carneiro Girão aff002; Rodolfo de Melo Nunes aff001; Ana Carolina Matias Dinelly Pinto aff001; Bruno Vidal aff003; João Eurico Fonseca aff003
Působiště autorů:
Departmento de Morfologia, Faculdade de Medicina, Universidade Federal do Ceará, Fortaleza, Ceará, Brasil
aff001; Department of Internal Medicine, Faculty of Medicine, Federal University of Ceará, Fortaleza, Ceará, Brazil
aff002; Serviço de Reumatologia e Doenças Ósseas Metabólicas, Hospital de Santa Maria, CHULN, Lisboa, Portugal
aff003; Unidade de Investigação em Reumatologia, Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, Centro Académico de Medicina de Lisboa, Portugal
aff004
Vyšlo v časopise:
PLoS ONE 14(12)
Kategorie:
Research Article
doi:
https://doi.org/10.1371/journal.pone.0226986
Souhrn
Purpose
To demonstrate the production of inflammatory mediators by cells located in bone marrow spaces inside rodent menisci.
Methods
Mice subjected to transection of the medial collateral and anterior cruciate ligaments and meniscotomy (osteoarthritis model) or to a sham procedure, as well as non-operated (naive) mice and rats, had knee joints excised. Tissues were stained with hematoxylin-eosin and tartrate-resistant acid phosphatase (TRAP). CD68+ cells, inducible nitric oxide synthase (iNOS), interleukin (IL)-1β, and tumor necrosis factor (TNF) expression were detected using immunohistochemistry.
Results
Lamellar ossified areas, bone-entrapped osteocytes and bone marrow spaces were found inside menisci of one week up to 6 months-old naïve mice, regardless of gender. Menisci from naive rats also showed the same pattern with bone marrow areas. CD68+ cells were identified in bone marrow areas inside the meniscus of mice. TRAP+ osteoclasts, and hematogenous precursors expressing IL-1β, TNF, and iNOS were identified inside bone marrow areas in meniscal samples from both naïve and sham operated mice. Quantitative immunoexpression of IL-1 β, TNF and iNOS was more intense, P = 0.0194, 0.0293, 0.0124, respectively, in mouse knees from mice sacrificed 49 days after being subjected to an osteoarthritis (OA) model as compared to sham operated animals.
Conclusion
We provide novel data showing that rodent menisci display bone marrow areas with cells able to produce inflammatory mediators. Immunoexpression of inflammatory mediators in those bone marrow areas is significantly more pronounced in mice subjected to experimental OA
Klíčová slova:
Bone marrow – Bone marrow cells – Cell staining – Cytokines – Inflammation – Knee joints – Osteoarthritis – Skeletal joints
Zdroje
1. Englund M, Roemer FW, Hayashi D, Crema MD, Guermazi A. Meniscus pathology, osteoarthritis and the treatment controversy. Nat Rev Rheumatol. 2012; 8:412–9. doi: 10.1038/nrrheum.2012.69 22614907
2. Englund M, Haugen IK, Guermazi A, Roemer FW, Niu J, Neogi T, et al. Evidence that meniscus damage may be a component of osteoarthritis: the Framingham study. Osteoarthritis Cartilage. 2016; 24:270–3. doi: 10.1016/j.joca.2015.08.005 26318660
3. Roemer FW, Kwoh CK, Hannon MJ, Hunter DJ, Eckstein F, Fujii T, et al. What comes first? Multitissue involvement leading to radiographic osteoarthritis: magnetic resonance imaging-based trajectory analysis over four years in the osteoarthritis initiative. Arthritis Rheumatol. 2015; 67:2085–96. doi: 10.1002/art.39176 25940308
4. McAlindon TE. Toward a new paradigm of knee osteoarthritis. Arthritis Rheumatol. 2015; 67:1987–9. doi: 10.1002/art.39177 25940432
5. Makris EA, Hadidi P, Athanasiou KA. The knee meniscus: structure-function, pathophysiology, current repair techniques, and prospects for regeneration. Biomaterials. 2011; 32:7411–31. doi: 10.1016/j.biomaterials.2011.06.037 21764438
6. Verdonk PC, Forsyth RG, Wang J, Almqvist KF, Verdonk R, Veys EM, et al. Characterisation of human knee meniscus cell phenotype. Osteoarthritis Cartilage. 2005;13;548–60. doi: 10.1016/j.joca.2005.01.010 15979007
7. Fuhrmann IK, Steinhagen J, Rüther W, Schumacher U. Comparative immunohistochemical evaluation of the zonal distribution of extracellular matrix and inflammation markers in human meniscus in osteoarthritis and rheumatoid arthritis. Acta Histochem. 2015; 117:243–54. doi: 10.1016/j.acthis.2014.12.009 25827912
8. Stone AV, Loeser RF, Vanderman KS, Long DL, Clark SC, Ferguson CM. Pro-inflammatory stimulation of meniscus cells increases production of matrix metalloproteinases and additional catabolic factors involved in osteoarthritis pathogenesis. Osteoarthritis Cartilage. 2014; 22:264–74. doi: 10.1016/j.joca.2013.11.002 24315792
9. Gupta T, Zielinska B, McHenry J, Kadmiel M, Haut Donahue TL. IL-1 and iNOS gene expression and NO synthesis in the superior region of meniscal explants are dependent on the magnitude of compressive strains.Osteoarthritis Cartilage. 2008; 16:1213–9. doi: 10.1016/j.joca.2008.02.019 18439846
10. Gamer LW, Xiang L, Rosen V. Formation and maturation of the murine meniscus. J Orthop Res. 2016 Sep 24. doi: 10.1002/jor.23446 27664939
11. de Melo Leite AC, Teotonio MA, Girão VC, de Lima Pompeu MM, de Melo Nunes R, Cunha TM, et al. Meniscal transection rather than excision increases pain behavior and structural damage in experimental osteoarthritis in mice. Osteoarthritis Cartilage. 2014;22;1878–85. doi: 10.1016/j.joca.2014.07.019 25086402
12. Kapadia RD, Badger AM, Levin JM, Swift B, Bhattacharyya A, Dodds RA, et al. Meniscal ossification in spontaneous osteoarthritis in the guinea-pig. Osteoarthritis Cartilage. 2000; 8:374–7. doi: 10.1053/joca.1999.0312 10966844
13. Kato Y, Oshida M, Saito A, Ryu J. Meniscal ossicles. J Orthop Sci. 2007; 12:375–80. doi: 10.1007/s00776-007-1140-3 17657558
14. Weischenfeldt J, Porse B. Bone Marrow-Derived Macrophages (BMM): Isolation and Applications. B. CSH Protoc. 2008 Dec 1; 2008:pdb.prot5080.
15. Berenbaum F. Osteoarthritis as an inflammatory disease (osteoarthritis is not osteoarthrosis!). Osteoarthritis Cartilage. 2013; 21:16–21. doi: 10.1016/j.joca.2012.11.012 23194896
16. Abramson SB. Osteoarthritis and nitric oxide. Osteoarthritis Cartilage 2008. 16 Suppl 2:S15–20.
17. Del Carlo M Jr, Loeser RF. Cell death in osteoarthritis. Curr Rheumatol Rep. 2008; 10:37–42. doi: 10.1007/s11926-008-0007-8 18457610
18. da Rocha FA, de Brum-Fernandes AJ. Evidence that peroxynitrite affects human osteoblast proliferation and differentiation. J Bone Miner Res. 2002;17:434–42. doi: 10.1359/jbmr.2002.17.3.434 11874235
19. Mathiessen A, Conaghan PG. Synovitis in osteoarthritis: current understanding with therapeutic implications. Arthritis Res Ther. 2017; 19:18. doi: 10.1186/s13075-017-1229-9 28148295
20. McInnes IB, Schett G. Pathogenetic insights from the treatment of rheumatoid arthritis. Lancet. 2017; 389:2328–37. doi: 10.1016/S0140-6736(17)31472-1 28612747
21. Poole R, Blake S, Buschmann M, Goldring S, Laverty S, Lockwood S et al. Recommendations for the use of preclinical models in the study and treatment of osteoarthritis. Osteoarthritis Cartilage. 2010;18:S10–16. doi: 10.1016/j.joca.2010.05.027 20864015
22. Lebel B, Tardieu C, Locker B, Hulet C. Ontogeny-Phylogeny. In: The Meniscus, Beaufils P & Verdonk R. Ed. Springer-Verlag Berlin Heidelberg 2010:3–9.
23. Little CB, Zaki S. What constitutes an "animal model of osteoarthritis"—the need for consensus? Osteoarthritis Cartilage. 2012; 20:261–7. doi: 10.1016/j.joca.2012.01.017 22321719
Článek vyšel v časopise
PLOS One
2019 Číslo 12
- S diagnostikou Parkinsonovy nemoci může nově pomoci AI nástroj pro hodnocení mrkacího reflexu
- Je libo čepici místo mozkového implantátu?
- Pomůže v budoucnu s triáží na pohotovostech umělá inteligence?
- AI může chirurgům poskytnout cenná data i zpětnou vazbu v reálném čase
- Nová metoda odlišení nádorové tkáně může zpřesnit resekci glioblastomů
Nejčtenější v tomto čísle
- Methylsulfonylmethane increases osteogenesis and regulates the mineralization of the matrix by transglutaminase 2 in SHED cells
- Oregano powder reduces Streptococcus and increases SCFA concentration in a mixed bacterial culture assay
- The characteristic of patulous eustachian tube patients diagnosed by the JOS diagnostic criteria
- Parametric CAD modeling for open source scientific hardware: Comparing OpenSCAD and FreeCAD Python scripts
Zvyšte si kvalifikaci online z pohodlí domova
Všechny kurzy