A partial genome assembly of the miniature parasitoid wasp, Megaphragma amalphitanum

English version

Autoři: Fedor S. Sharko ^aff001; Artem V. Nedoluzhko ^aff002; Brandon M. Lê ^aff004; Svetlana V. Tsygankova ^aff002; Eugenia S. Boulygina ^aff002; Sergey M. Rastorguev ^aff002; Alexey S. Sokolov ^aff001; Fernando Rodriguez ^aff004; Alexander M. Mazur ^aff001; Alexey A. Polilov ^aff005; Richard Benton ^aff006; Michael B. Evgen'ev ^aff007; Irina R. Arkhipova ^aff004; Egor B. Prokhortchouk ^aff001; Konstantin G. Skryabin ^aff001
Působiště autorů: Institute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences, Moscow, Russia ^aff001; National Research Center “Kurchatov Institute”, Moscow, Russia ^aff002; Nord University, Faculty of Biosciences and Aquaculture, Bodø, Norway ^aff003; Josephine Bay Paul Center for Comparative Molecular Biology and Evolution, Marine Biological Laboratory, Woods Hole, Massachusetts, United States of America ^aff004; Lomonosov Moscow State University, Faculty of Biology, Moscow, Russia ^aff005; Center for Integrative Genomics, Faculty of Biology and Medicine, University of Lausanne, Lausanne, Switzerland ^aff006; Institute of Molecular Biology RAS, Moscow, Russia ^aff007
Vyšlo v časopise: PLoS ONE 14(12)
Kategorie: Research Article
doi: https://doi.org/10.1371/journal.pone.0226485

Souhrn

Body size reduction, also known as miniaturization, is an important evolutionary process that affects a number of physiological and phenotypic traits and helps animals conquer new ecological niches. However, this process is poorly understood at the molecular level. Here, we report genomic and transcriptomic features of arguably the smallest known insect–the parasitoid wasp, Megaphragma amalphitanum (Hymenoptera: Trichogrammatidae). In contrast to expectations, we find that the genome and transcriptome sizes of this parasitoid wasp are comparable to other members of the Chalcidoidea superfamily. Moreover, compared to other chalcid wasps the gene content of M. amalphitanum is remarkably conserved. Intriguingly, we observed significant changes in M. amalphitanum transposable element dynamics over time, in which an initial burst was followed by suppression of activity, possibly due to a recent reinforcement of the genome defense machinery. Overall, while the M. amalphitanum genomic data reveal certain features that may be linked to the unusual biological properties of this organism, miniaturization is not associated with a large decrease in genome complexity.

Klíčová slova:

Comparative genomics – Genome analysis – Genomic libraries – Genomic library construction – Genomics statistics – Insects – Invertebrate genomics – Transcriptome analysis

Zdroje

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A partial genome assembly of the miniature parasitoid wasp, Megaphragma amalphitanum

Souhrn

Klíčová slova:

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