Genome-wide identification and expression analysis of the PHD-finger gene family in Solanum tuberosum

English version

Autoři: Mingyue Qin ^aff001; Wenbin Luo ^aff004; Yan Zheng ^aff001; Huazhong Guan ^aff002; Xiaofang Xie ^aff001
Působiště autorů: College of Life Sciences, Fujian Agriculture & Forestry University, Fuzhou, China ^aff001; Key Laboratory for Genetics, Breeding and Multiple Utilization of Crops, Ministry of Education, Fujian Agriculture & Forestry University, Fuzhou, China ^aff002; Fujian Key Laboratory of Crop Breeding by Design, Fujian Agriculture & Forestry University, Fuzhou, China ^aff003; The Crop Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian, China ^aff004
Vyšlo v časopise: PLoS ONE 14(12)
Kategorie: Research Article
doi: https://doi.org/10.1371/journal.pone.0226964

Souhrn

Plant homeodomain (PHD) proteins are prevalent in eukaryotes and play important roles in plant growth, development and abiotic stress response. In this study, the comprehensive study of the PHD family (StPHD) was performed in potato (Solanum tuberosum L.). Seventy-two PHD genes (named StPHD1-72) were identified and grouped into 10 subfamilies based on phylogenetic analysis. Similar structure organizations were found within each subfamily according to the exon/intron structures and protein motif analysis. These genes were unequally scattered on the chromosomes of potato, with 9 pairs of segmental duplicated genes and 6 pairs of tandem duplicated genes showing that both segmental duplicated and tandem duplicated events contributed to the expansion of the potato PHD family. The gene ontology (GO) analysis suggests that StPHD mainly functioned at the intracellular level and was involved in various binding, metabolic and regulation processes. The analysis of expression patterns of StPHD genes showed that these genes were differentially expressed in 10 different tissues and responded specifically to heat, salt and drought stress based on the FPKM (Fragments per kilobase of transcript per million mapped reads) values of the RNA-seq data. Furthermore, the real-time quantitative PCR for 12 selected StPHD genes revealed the various levels of gene expression corresponding to abiotic stress. Our results provide useful information for a better understanding of PHD genes and provide the foundation for additional functional exploration of the potato PHD gene family.

Klíčová slova:

Arabidopsis thaliana – Gene expression – Maize – Phylogenetic analysis – Potato – Sequence alignment – Sequence motif analysis – Duplicated genes

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Genome-wide identification and expression analysis of the PHD-finger gene family in Solanum tuberosum

Souhrn

Klíčová slova:

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