Tissue Expression Pattern of PMK-2 p38 MAPK Is Established by the miR-58 Family in
MicroRNAs are small, noncoding RNAs that act post-transcriptionally to inhibit expression of their target mRNAs. Gene expression studies of microRNAs and their target transcripts in diverse organisms have suggested that microRNAs may function to shape patterns of tissue expression. In this paper, we show that the miR-58/80-82 family of microRNAs, which accounts for roughly half of all C. elegans microRNAs at all developmental stages, defines the spatial expression pattern of PMK-2 p38 MAPK. While the pmk-2 gene is broadly transcribed, its tissue-specific expression is established by the redundant activities of miR-58, miR-80, miR-81, and miR-82, which switch off expression of PMK-2 through destabilization of pmk-2 mRNA in non-neuronal tissues. Our data suggest a housekeeping role for the miR-58/80-82 family in establishing and maintaining neuronal patterns of gene expression in C. elegans, and supports a more general role for microRNAs in establishing patterns of tissue expression.
Published in the journal:
. PLoS Genet 11(2): e32767. doi:10.1371/journal.pgen.1004997
Category:
Research Article
doi:
https://doi.org/10.1371/journal.pgen.1004997
Summary
MicroRNAs are small, noncoding RNAs that act post-transcriptionally to inhibit expression of their target mRNAs. Gene expression studies of microRNAs and their target transcripts in diverse organisms have suggested that microRNAs may function to shape patterns of tissue expression. In this paper, we show that the miR-58/80-82 family of microRNAs, which accounts for roughly half of all C. elegans microRNAs at all developmental stages, defines the spatial expression pattern of PMK-2 p38 MAPK. While the pmk-2 gene is broadly transcribed, its tissue-specific expression is established by the redundant activities of miR-58, miR-80, miR-81, and miR-82, which switch off expression of PMK-2 through destabilization of pmk-2 mRNA in non-neuronal tissues. Our data suggest a housekeeping role for the miR-58/80-82 family in establishing and maintaining neuronal patterns of gene expression in C. elegans, and supports a more general role for microRNAs in establishing patterns of tissue expression.
Introduction
Since the initial genetic identification of microRNAs in Caenorhabditis elegans [1,2], biochemical cloning methods and computational approaches have identified hundreds of microRNAs [3,4], though genetic analysis has defined functional roles for relatively few of these [5,6]. A single microRNA, miR-58, constitutes nearly half of all microRNAs in C. elegans, with constitutive expression in non-neuronal tissues through all developmental stages [7,8]. Differential RNA binding protein (RBP) immunoprecipitation with subsequent mRNA and protein quantification analyses (RIP-chip-SRM) has indicated the presence of hundreds of miR-58 targets [9]. Whereas deletion of miR-58 does not cause any apparent defects, a strain carrying deletions in the miR-58 family, comprised of miR-58 and the homologous microRNAs miR-80, miR-81, and miR-82, exhibits multiple mutant phenotypes, including defects in size, locomotion, and reproductive egg-laying [6].
Three C. elegans genes with homology to mammalian p38 MAPK—pmk-1, pmk-2, and pmk-3—are in a single operon along with an additional upstream gene, islo-1 (Fig. 1A). PMK-1 and PMK-2 are highly homologous, sharing a 62% amino acid sequence identity and have the signature TGY motif found in the activation loop of p38 MAPKs [10]. PMK-1 regulates innate immunity in the intestine of C. elegans and is activated by a MAPK signaling cassette composed of p38 MAPK kinase SEK-1 and the MAPKKK NSY-1, homologous to mammalian MKK3/6 and ASK1, respectively [11,12]. Functioning upstream of NSY-1 and required for activation of PMK-1 in C. elegans is TIR-1, a conserved Toll-Interleukin-1 Receptor domain adaptor protein orthologous to mammalian SARM [13,14]. TIR-1-NSY-1-SEK-1 functions in the nervous system to regulate the specification of neuronal asymmetry in the AWC neuron pair [15–17], reproductive egg-laying behavior, and the upregulation of serotonin biosynthesis in the ADF chemosensory neurons in response to infection by Pseudomonas aeruginosa [12], but the MAPK targeted in the nervous system for these processes has not been defined, with pmk-1 loss-of-function not affecting these neuronal phenotypes.
Here, we show that PMK-2 functions redundantly with PMK-1 in the nervous system of C. elegans to regulate development and behavioral responses to pathogenic bacteria, whereas PMK-1 alone functions in the intestine to regulate innate immunity. We observe distinct tissue expression patterns for the co-operonic pmk-1 and pmk-2 genes; in contrast to the ubiquitous expression pattern of PMK-1, PMK-2 is largely restricted to the nervous system. Tissue-specific expression of PMK-2 is established by the miR-58 family, which switches off expression of PMK-2 in non-neuronal tissues. Our data suggest a role for the relatively abundant miR-58 microRNA in the establishment of tissue-specific gene expression in C. elegans.
Results
Tissue-specific activities of p38 MAPK genes in C. elegans
We used mutant alleles of pmk-1 and pmk-2 to confirm that PMK-1 alone is required for expression of an intestinal reporter for p38 MAPK activity and innate immunity to infection by P. aeruginosa in the intestine. The agIs219 reporter transgene contains the green fluorescent protein (GFP) gene fused to the promoter of the PMK-1-regulated gene T24B8.5, which is predicted to encode a peptide homologous to ShK toxin peptides, and serves as an in vivo readout of p38 MAPK activity in the intestine [12]. Expression of agIs219 in the intestine is extremely diminished in the pmk-1(km25) mutant (Fig. 1B). In contrast, expression of agIs219 is unchanged in pmk-2(qd284) and pmk-2(qd287) mutant animals (Fig. 1B). Similarly, in contrast to the enhanced susceptibility to P. aeruginosa observed in pmk-1(km25) mutant animals, pmk-2(qd284) and pmk-2(qd287) mutant animals display a normal innate immune response to P. aeruginosa (Fig. 1C).
To evaluate the roles of PMK-1 and PMK-2 in mediating the activities of the TIR-1-NSY-1-SEK-1 signaling module in the nervous system, we utilized two assays of neuronal signaling processes that are dependent on TIR-1-NSY-1-SEK-1. First, the establishment of asymmetry in the AWC neurons during development is a stochastic process for which expression of a transgenic reporter, str-2::GFP, in one AWC neuron or the other serves as a readout [17]. TIR-1-NSY-1-SEK-1 signaling represses the expression of str-2::GFP in one AWC neuron such that in a wild type animal, only one AWC neuron expresses str-2::GFP. Strains carrying loss-of-function mutations in the TIR-1-NSY-1-SEK-1 signaling module express str-2::GFP in both AWC neurons [15–17]. We observed expression of str-2::GFP in only one AWC neuron in pmk-1 and pmk-2 loss-of-function single mutants (Fig. 1D). In contrast, using two strains carrying loss-of-function mutations in both genes—pmk-2(qd279 qd171) pmk-1(km25) and pmk-2(qd280 qd171) pmk-1(km25)—we observed expression of str-2::GFP in both AWC neurons, similar to what is observed in the sek-1(km4) mutant (Fig. 1D). A second process that is dependent on TIR-1-NSY-1-SEK-1 activity in the nervous system is the increased expression of tph-1, which encodes the serotonin biosynthetic enzyme tryptophan hydroxylase, in the ADF chemosensory neurons upon exposure of C. elegans to pathogenic P. aeruginosa [12,18]. Upregulation of serotonin levels in the ADF neuron pair has been implicated in aversive learning behavior to pathogenic bacteria [18]. We observed that PMK-1 and PMK-2 also function redundantly in the P. aeruginosa-induced expression of a Ptph-1::GFP transgene reporter in the ADF neurons (Fig. 1E). These data establish that while PMK-1 function alone is required to regulate intestinal innate immunity, PMK-1 and PMK-2 function redundantly in the nervous system downstream of the TIR-1-NSY-SEK-1 signaling module in neuronal developmental and pathogen-dependent responses (Fig. 1F).
We reasoned that the genetic redundancy of pmk-1 and pmk-2 in neurons, but not in the intestine, might be the result of differences in tissue expression of these genes. We proceeded to examine the tissue expression patterns for PMK-1 and PMK-2 by constructing a translational reporter for the pmk operon, qdEx101, consisting of upstream promoter sequence and the entire length of the operon with a GFP tag engineered onto the C-terminus of PMK-2 and a mCherry tag engineered onto the C-terminus of PMK-1 (Fig. 2A). We observed broad expression of PMK-1 in multiple tissue types, whereas expression of PMK-2 was mostly restricted to the nervous system; PMK-2 expression was detected in head, body, and tail ganglia as well as the nerve ring and ventral nerve cord (Fig. 2B). We also observed faint and diffuse expression of PMK-2 in the distal tip cell and spermatheca at levels much lower than observed in the nervous system. Importantly, PMK-2 expression was excluded from the intestine, where PMK-1 functions solely in innate immunity. These data suggest that the tissue-specific genetic redundancy of p38 MAPK signaling in C. elegans is a consequence of distinct tissue expression patterns of the co-transcribed pmk-1 and pmk-2 genes and implicate post-transcriptional mechanisms in the tissue-specific regulation of pmk-2 expression.
The 3’UTR of pmk-2 regulates mRNA stability
Insight into the post-transcriptional regulatory mechanism underlying the restricted tissue expression of PMK-2 came from a genetic screen for suppressors of the immunocompromised phenotype of a pmk-1 deletion mutant [19], in which we serendipitously isolated a gain-of-function mutant of pmk-2, qd171, containing a 913 bp insertion/184 bp deletion located in the 3’UTR of pmk-2 (Fig. 1A). The starting strain for the screen carried the km25 deletion allele of pmk-1 (Fig. 1A) and the agIs219 integrated GFP reporter transgene [12,19] (Fig. 3A). The pmk-2(qd171) deletion suppressed the diminished intestinal GFP expression from the agIs219 reporter transgene (Fig. 3A) and the enhanced pathogen susceptibility of the pmk-1(km25) mutant (Fig. 3B). RNAi of sek-1 and RNAi of pmk-2, reverted the pmk-2(qd171) pmk-1(km25) pathogen resistance and agIs219 intestinal GFP expression phenotypes associated with suppression of pmk-1 loss-of-function (Fig. 3A-3B). These data suggest that the ability of the pmk-2(qd171) mutation to suppress pmk-1 loss-of-function is dependent on PMK-2.
Because the location of the qd171 insertion/deletion in the 3’UTR of pmk-2 might be anticipated to influence mRNA stability, we proceeded to measure levels of pmk-2 mRNA in the pmk-2(qd171) pmk-1(km25) mutant. We detected a 5.7-fold increase in pmk-2 mRNA levels in the pmk-2(qd171) pmk-1(km25) mutant compared to wild type worms (Fig. 3C). The effect of increased mRNA levels due to the qd171 mutation is specific to pmk-2 and not a general characteristic of the pmk operon, as pmk-3 mRNA levels did not change in the pmk-2(qd171) pmk-1(km25) mutant relative to wild type. We next determined levels of activated PMK-2 protein in the pmk-2(qd171) pmk-1(km25) mutant using an antibody that recognizes the dually phosphorylated TGY motif in the activation domain of activated mammalian p38 MAPK and that is cross-reactive with C. elegans PMK-1 and PMK-2 [11]. Corroborating the increase in mRNA levels, we detected at least a 5.7-fold increase in activated PMK-2 protein levels in the pmk-2(qd171) pmk-1(km25) mutant (Fig. 3D).
To verify that the increase in levels of pmk-2 mRNA and activated protein was due to the 3’UTR deletion of pmk-2 in the pmk-2(qd171) pmk-1(km25) mutant, we used CRISPR-Cas-9-mediated genome editing to engineer the wild type N2 strain to carry a deletion in the 3’UTR of pmk-2. We obtained a 144 bp deletion, qd305, in the 3’UTR of pmk-2 (S1A Fig.). We proceeded to measure levels of pmk-2 mRNA and activated protein in the pmk-2(qd305) mutant and detected a 7.4-fold increase in pmk-2 mRNA levels compared to wild type worms (S1B Fig.) and a corresponding increase in activated PMK-2 protein levels (S1C Fig.).
Taken together, these data suggest that a deletion in the 3’UTR of the gene encoding PMK-2 p38 MAPK can suppress the immunodeficient phenotype conferred by loss-of-function of pmk-1 through increased stability of pmk-2 mRNA and levels of activated PMK-2 protein.
The miR-58 family restricts expression of PMK-2 in C. elegans
We next sought to define the cis-regulatory determinants of the pmk-2 3’UTR that function to repress PMK-2 expression. The region deleted in the qd171 allele of pmk-2 contains the most distal polyadenylation signal (PAS) used in 3’-end formation of pmk-2 mRNA. We performed 3’ RACE on pmk-2 mRNA isolated from wild type and pmk-2(qd171) pmk-1(km25) mutant animals to determine the effect of the qd171 mutation on the length of the 3’UTR of pmk-2 mRNA. Sequencing of the 3’ RACE products revealed use of a more proximal PAS leading to a 206 bp truncation in the 3’UTR of pmk-2 mRNA in the pmk-2(qd171) pmk-1(km25) mutant (Fig. 4A). We examined the truncated region for cis-regulatory elements conserved among Caenorhabditis species and identified three seed match sites for the miR-58/80-82 family of microRNAs residing in this region absent from the 3’UTR of pmk-2 mRNA in the pmk-2(qd171) pmk-1(km25) mutant (Fig. 4A). These miR-58/80-82 seed match sites are also absent in the pmk-2(qd305) mutant (S1A Fig.).
The miR-58/80-82 family consists of miR-58, miR-80, miR-81, miR-82, and miR-1834. Mutants carrying deletions in mir-58, mir-80, and mir-81-82 [5] were used to assess whether the miR-58/80-82 family functions to repress the expression of pmk-2. Loss of any individual mir-58/80-82 family member had no effect on pmk-2 mRNA levels relative to wild type (Fig. 4B). However, loss of both mir-58 and mir-80 resulted in a 3-fold increase in pmk-2 mRNA levels relative to wild type, and loss of mir-58/80-82 led to an even further increase in pmk-2 mRNA to a level 6.3-fold greater than wild type (Fig. 4B), without altering levels of pmk-3 mRNA. Corroborating the mRNA analysis, we observed at least similar increases in activated PMK-2 protein levels in both mir-80; mir-58 and mir-80; mir-58; mir-81-82 mutants, but not in other mutants (Fig. 4C). These data suggest that the miR-58/80-82 family acts redundantly to destabilize pmk-2 mRNA.
The observation that the pmk-2(qd171) mutation suppresses the loss of pmk-1 function in the intestine suggested that not only are PMK-2 levels increased in the pmk-2(qd171) pmk-1(km25) mutant, but that the site of expression had also changed. To determine the spatial effect on expression of releasing pmk-2 from regulation by the miR-58/80-82 family, we engineered a new pmk operon translational reporter, qdEx102, which carries mutations in the second and third miR-58/80-82 seed match sites [20] in the 3’UTR of pmk-2 (Fig. 4A). Expression of PMK-1 was unchanged, whereas expression of PMK-2 was detected in many additional cell types including the intestine, body wall muscle, pharyngeal muscle, hypodermis, and vulva (Fig. 2C). Expression of PMK-2 in the distal tip cell and spermatheca, which was faint and diffuse with miR-58/80-82 regulation intact (Fig. 2B), was markedly elevated (Fig. 2C). Similar misexpression of pmk-2 was observed when we crossed qdEx101 (pmk operon reporter with miR-58/80-82 seed match sites intact) into the mir-80; mir-58; mir-81-82 mutant (Fig. 2D). These data suggest that the miR-58/80-82 family of microRNAs restricts expression of PMK-2 p38 MAPK through the post-transcriptional destabilization of pmk-2 mRNA in non-neuronal tissues (Fig. 4D).
Discussion
Our data on the tissue-specific genetic redundancy of p38 MAPK signaling in C. elegans define a role for the relatively abundant and constitutively expressed miR-58/80-82 family of microRNAs in establishing the tissue-specific expression of PMK-2 p38 MAPK. We show that although the pmk-2 gene is widely transcribed, PMK-2 protein is found nearly exclusively in the nervous system of C. elegans where PMK-2 functions redundantly with PMK-1 to regulate neuronal development and behavioral responses to pathogenic bacteria. We determined that the tissue-specific expression of PMK-2 is dependent on cis-regulatory sequences found within its 3’UTR and demonstrated that the miR-58/80-82 family is required to switch off expression of PMK-2 in non-neuronal tissues post-transcriptionally, thereby establishing its tissue-specific expression in C. elegans.
Genome wide analyses of microRNA expression in C. elegans using transgenes containing the putative promoters of microRNAs fused to the gene encoding green fluorescent protein have been reported previously [8,21]. mir-58 was inferred to be expressed in the intestine, hypodermis, pharynx, spermatheca, excretory canal, and excretory cell soma [8]. Expression of mir-58 was not observed in the nervous system. mir-80 was shown to be expressed in the posterior intestine, head and body wall muscle, uterus, vulva, distal tip cells, excretory cells, dorsal nerve cord, and amphid neurons; mir-81 was shown to be expressed weakly in head neurons; and mir-82 was observed to be expressed in pharyngeal muscle, spermatheca, and a subset of both the ventral nerve cord and the amphid neurons [21]. The tissues in which these microRNAs are reportedly present are consistent with the absence of PMK-2 expression we observe in these tissues when miR-58/80-82 regulation is intact (Fig. 2B). Additionally, these data are consistent with the tissues that misexpress PMK-2 when miR-58/80-82 regulation is disabled (Fig. 2C-2D). Taken together with our data, these reported expression data on the miR-58/80-82 family suggest that the tissue-specific expression of PMK-2 is a consequence of distinct tissue-expression patterns of the corresponding microRNAs that target pmk-2.
MicroRNAs have been implicated to function both as a backup to reinforce transcriptional gene programs and as an instructive signal to shape gene expression patterns [22]. Expression analyses of microRNAs and their putative target transcripts in Drosophila and mammals showed largely nonoverlapping expression patterns, suggesting that microRNAs function secondarily to transcriptional mechanisms to reinforce promoter-defined spatial expression patterns of their targets at the post-transcriptional level [23–25]. Studies in zebrafish revealed overlapping expression patterns and observed that levels of transcripts are often lower in cells expressing the microRNA, leading to the hypothesis that microRNAs may also play a more active role in conjunction with transcriptional mechanisms to shape gene expression patterns of their targets [26]. Supporting this hypothesis is the genetic analysis of muscle microRNAs miR-1 and miR-133 in zebrafish, which were shown to refine the expression levels of their target transcripts in muscle [27]. We show nonoverlapping expression of PMK-2 and miR-58/80-82, established not by the promoter of these genes, but rather complete destabilization of pmk-2 mRNA by miR-58/80-82, suggesting that the activity of microRNAs can define specific patterns of gene expression in different cell types.
Promoter and enhancer elements commonly direct tissue-specific gene expression in animals, and thus a role for miR-58 family-mediated post-transcriptional regulation in establishing the tissue expression pattern of pmk-2 might be somewhat unexpected. For pmk-2, microRNA-mediated regulation may in part be a consequence of the co-transcription of both pmk-2 and pmk-1 from the same operon. Genes co-transcribed in eukaryotic operons need not share a similarity in function and are generally not thought to be expressed in specific tissues [28]. Our data suggest that microRNA-mediated regulation may contribute to differential tissue expression patterns of genes co-transcribed from the same operon in C. elegans.
MicroRNAs have been shown to establish cell fate through specific cell type expression during C. elegans development [29–31]. For example, the lsy-6 microRNA regulates the development of left/right asymmetry through the specific repression of its target in one of two ASE chemosensory neurons [29]. In contrast to the highly selective expression and function of the lsy-6 microRNA in a pair of chemosensory neurons, the miR-58 family functions in a large number of cells and tissues to restrict expression of PMK-2 in non-neuronal tissues at all developmental stages.
The systematic identification of microRNAs of C. elegans by deep sequencing determined that miR-58 is the most abundant microRNA, accounting for nearly half of all microRNAs present in C. elegans at all developmental stages [7]. The abundance of miR-58, along with the presence of multiple miR-58 binding sites in the 3’UTR of pmk-2, may contribute to the large increase of pmk-2 mRNA and protein levels when miR-58 family regulation is inhibited. The approximately six-fold difference we observe in pmk-2 mRNA levels and comparable difference in PMK-2 protein levels reflect quantitation from whole worm lysates in which PMK-2 is detectably expressed in the nervous system. Considering this and the lack of PMK-2 protein observed in non-neuronal tissues when miR-58 targeting is intact (i.e. wild type conditions), the magnitude of PMK-2 repression in non-neuronal tissues is likely much greater than six-fold. The switch-like “off” state of PMK-2 expression imposed by the miR-58/80-82 family in non-neuronal tissues in regulating the spatial expression of PMK-2 is reminiscent of the magnitude of target repression exhibited by lin-4 and let-7 microRNAs in the temporal control of developmental timing [2,32]. In addition, our data corroborate prior phenotypic analysis suggestive of redundancy among members of the miR-58 family [5,6], demonstrating redundant roles for miR-58, miR-80, miR-81, and miR-82 in the destabilization of pmk-2 mRNA and corresponding repression of activated PMK-2 protein levels.
The broad and constitutive expression of mir-58/80-82 suggests a more general housekeeping role for this microRNA family in the establishment and maintenance of tissue-specific gene expression by repressing the expression of neuronal-specific genes in non-neuronal tissues. Supporting this hypothesis is the genome-wide analysis of tissue-specific gene expression in C. elegans, which revealed enrichment for miR-58 binding sites among neuronal genes [33]. We speculate that the defects in size, locomotion, and egg-laying behavior observed in the mir-58; mir-80; mir-81-82 mutant are due to the cumulative misexpression of miR-58/80-82 target genes in non-neuronal tissues, as pmk-2 loss-of-function alone cannot suppress these defects (D.J.P. and D.H.K., unpublished observations).
The characterization of microRNAs expressed in specific tissues of mice revealed the presence of a single microRNA and/or microRNA family in high abundance [34], raising the possibility that such microRNAs might function to establish tissue expression patterns. Our data on the regulation of PMK-2 tissue expression by the miR-58 family provide genetic evidence for this hypothesis and point to a more general role for the highly abundant miR-58 family in the maintenance of tissue-specific gene expression.
Materials and Methods
Strains
All C. elegans strains were maintained and propagated on E. coli OP50 as described previously [35]. N2 was the wild-type strain. The following mutations were used in this study:
LGI: kyIs140[str-2::GFP, lin-15(+)]
LGIII: agIs219[PT24B8.5::GFP, Pttx-3::GFP], tir-1(qd4), mir-80(nDf53)
LGIV: mir-58(n4640), pmk-2(qd284), pmk-2(qd307), pmk-2(qd287), pmk-2(qd279), pmk-2(qd280), pmk-2(qd305), pmk-2(qd171), pmk-1(km25)
LGX: mir-81-82(nDf54), sek-1(km4), nIs145[Ptph-1::GFP, lin-15(+)]
Extrachromosomal arrays: qdEx101[Poperon::islo-1::pmk-3::pmk-2::GFP::pmk-1::mCherry], qdEx102[Poperon::islo-1::pmk-3::pmk-2mut::GFP::pmk-1::mCherry]
A list of all strains used in this study is provided in the Supporting Information (S1 Table).
Pathogenesis assays
Cultures of P. aeruginosa PA14 were grown in Luria-Bertani (LB) broth overnight at 37°C. Five microliters of the overnight culture was used to seed 35-mm slow-kill assay plates (1.7% agar, 0.35% peptone, 0.3% sodium chloride, 5 μg/ml cholesterol, 1 mM calcium chloride, 1 mM magnesium sulfate, 25 mM potassium phosphate) containing 50 μg/ml 5-fluorodeoxyuridine (FUdR), used to prevent eggs from hatching. The culture was seeded in the middle of the plates and was not spread to the edges, meaning the resulting lawn would be “small,” allowing for behavioral avoidance. The seeded plates were incubated overnight at 37°C and then overnight at room temperature. Roughly 40 L4 larval stage worms were placed onto a plate containing the prepared P. aeruginosa bacterial lawn with four plates per strain. The assay was carried out at 25°C. Plates were checked at the indicated times and worms that did not respond to a gentle prod from a platinum wire were scored as dead. Worms that crawled off of the plate or burrowed were censored.
Ptph-1::GFP imaging and quantification of GFP fluorescence
Adult worms (specifically, 12–16 hr post L4 larval stage at 25°C) were transferred to plates containing either a lawn of non-pathogenic E. coli OP50 or pathogenic P. aeruginosa PA14 and incubated at 25°C for 6 hr at which time the worms were immobilized with 50 mM sodium azide and mounted on 2% agarose pads. Immobilized worms were viewed using an AxioImager Z1 fluorescence microscope (Carl Zeiss AG, Oberkochen, Germany) with an EC Plan-Neofluar 40x/1.3 Oil DIC objective and the focal plane with the strongest GFP signal in the ADF neuron was used to take a 30 ms exposure picture with an AxioCam HRm camera. The images were analyzed in Fiji [36], where the ADF neuron was located and the pixel intensity values were examined. The maximum pixel intensity value in the ADF neuron was used as the Ptph-1::GFP fluorescence value for each worm. In each experiment, 7–10 worms of the indicated genotype were imaged for each condition (OP50, PA14). For the experiment shown in Fig. 1E, one outlier was identified (in the tir-1(qd4) mutant, P. aeruginosa exposure dataset) using Grubbs’ test and excluded from the graph and analysis. Statistical analyses of changes in fluorescence were performed in Prism 5 (GraphPad Software, Inc., La Jolla, CA) using a two-way ANOVA and Bonferroni post-test.
Isolation of pmk-2(qd284), pmk-2(qd287), and pmk-2(qd307)
The qd284, qd287, and qd307 alleles of pmk-2 were isolated by CRISPR-Cas9-mediated genome editing as described previously [37]. Two separate pmk-2 single guide RNA (sgRNA) expression vectors derived from pUC57 were constructed following the published protocol. Both sgRNAs target sequences located in what corresponds to the first exon of the pmk-2 transcript. The target sequences were chosen to contain restriction enzyme recognition sites to facilitate the screening for mutations. Germline transformation was performed as described previously [38] using the following plasmids: 50 ng/μl Peft-3::Cas9-SV40 NLS::tbb-2 3’UTR, 45 ng/μl PU6::pmk-2 sgRNA, 5 ng/μl pCFJ104[Pmyo-3::mCherry]. Screening for mutations was performed as outlined [37]. To confirm that a frameshift in the first exon of pmk-2 results in a null allele, the qd284 mutation was crossed into the mir-80; mir-58; mir-81-82 mutant and levels of activated PMK-2 protein were determined. Activated PMK-2 protein was not detected in the mir-80; mir-58 pmk-2(qd284); mir-81-82 mutant, indicating that these mutations are null (S2 Fig.).
Isolation of pmk-2(qd171)
The qd171 allele of pmk-2 was isolated from a screen for suppressors of the enhanced susceptibility to pathogen (Esp) phenotype conferred by the km25 loss-of-function allele of pmk-1. pmk-1(km25) mutant L4 larvae carrying the agIs219 reporter transgene for p38 activity in the intestine were mutagenized with ethyl methanesulfonate (EMS) as described previously [39]. Synchronized F2 progeny from roughly 35,000 mutagenized genomes were screened for an increase in expression of GFP from the agIs219 reporter transgene using a dissecting microscope equipped to detect GFP fluorescence. Mutants with increased expression of GFP were tested individually for the ability to suppress the Esp phenotype conferred by loss of pmk-1 function. Mutants that suppressed the Esp phenotype conferred by loss of pmk-1 function were then subsequently screened for suppression of the diminished expression of pmk-1 target genes conferred by loss of pmk-1 function. Single-nucleotide polymorphism (SNP)-based mapping using the C. elegans isolate CB4856 was performed as described previously [40,41]. One of the suppressors from this screen, qd171, mapped to a region on LGIV containing the pmk operon. Sequence determination of pmk-1 and pmk-2 revealed that qd171 was an allele of pmk-2.
Isolation of pmk-2(qd305)
The qd305 deletion allele of pmk-2 was isolated by CRISPR-Cas9-mediated genome editing as described previously [37]. Three separate pmk-2 sgRNA expression vectors derived from pUC57 were constructed following the published protocol. All three sgRNAs target sequences located within or directly downstream of the 3’UTR of pmk-2. Germline transformation was performed as described previously [38] using the following plasmids: 50 ng/μl Peft-3::Cas9-SV40 NLS::tbb-2 3’UTR, 50 ng/μl of each of the three PU6::pmk-2 3’UTR sgRNA, 5 ng/μl pCFJ104[Pmyo-3::mCherry]. Screening for deletions was performed by PCR analysis of F1 transgenic animals.
Isolation of pmk-2(qd279 qd171) and pmk-2(qd280 qd171)
The qd279 and qd280 alleles of pmk-2 were isolated from a screen for suppressors of the pmk-2(qd171) suppressor phenotype of the pmk-1(km25) loss-of-function phenotype. pmk-2(qd171) pmk-1(km25) mutant L4 larvae carrying the agIs219 reporter transgene for p38 activity in the intestine were mutagenized with EMS as described previously [39]. Synchronized F2 progeny from roughly 14,000 mutagenized genomes were screened for a decrease in expression of GFP from the agIs219 reporter transgene using a dissecting microscope equipped to detect GFP fluorescence. The sequence of pmk-2 was determined in mutants with diminished GFP expression from the agIs219 transgene. We identified three alleles of pmk-2: qd279 and qd280 (which are missense mutations in conserved residues and were utilized in this study), as well as qd281.
RNA isolation, 3’ RACE, and quantitative RT-PCR
Hypochlorite-synchronized populations of L4 larval stage worms were flash-frozen in liquid nitrogen and stored at -80°C until RNA extraction using TRI reagent (Ambion, Life Technologies, Thermo Fisher Scientific, Inc., Waltham, MA). Strain MT15563, carrying mutations in mir-58,80-82, grew slower than the other strains and therefore was harvested ~10 hr after the wild type strain. For 3’ RACE experiments, cDNA was prepared using the FirstChoice RLM-RACE Kit (Ambion). Two successive rounds of PCR were performed using nested primers specific to pmk-2 and an adaptor at the 3’-end. Sequence determination was performed by direct Sanger sequencing of 3’ RACE products. miR-58/80-82 seed match sites were identified using TargetScan [42]. For quantitative RT-PCR experiments, cDNA was prepared with the RETROscript Kit (Ambion) using oligo dT primers. qRT-PCR was performed with a Mastercycler Realplex (Eppendorf AG, Hamburg, Germany) with SYBR Green detection (Roche Diagnostics Corp., Indianapolis, IN) in triplicate 20 μl reactions. pmk-1, pmk-2, and pmk-3 mRNA levels were normalized to the control gene snb-1. Fold change relative to wild type was determined using the Pfaffl method [43]. Sequences of primers used for qRT-PCR: pmk-1, tgaatgatgatgtaagggcaga and cttcctcttcgtcagcaaatg; pmk-2, caagtgttacgtgggctcaa and cgagaatcttgacttcgcatc; pmk-3, gtatcgaagcaacgggaaac and tggaccacatggttttgaga; snb-1, ccggataagaccatcttgacg and gacgacttcatcaacctgagc.
Immunoblotting
For the experiments with pmk-1(km25), pmk-2(qd171) pmk-1(km25), and pmk-2(qd279 qd171) pmk-1(km25) strains with RNAi treatment (Fig. 3D), mixed stage populations of worms subjected to RNAi for multiple generations were used for Western analysis. For experiments with mir-58/80-82 family microRNA deletion strains (Fig. 4C and S2 Fig.) and pmk-2(qd305) (S1C Fig.), hypochlorite-synchronized populations of L4 larval worms were used for Western analysis. Strain MT15563, carrying mutations in mir-58,80-82, grew slower than the other strains and therefore was harvested ~10 hr after the wild type strain. Worms were collected, washed twice with M9, incubated in M9 while rotating at 20°C to clear the gut of bacteria, washed again twice with M9, and then pelleted. An equal volume of 2x lysis buffer (4% SDS, 100 mM Tris HCl pH 6.8, and 20% glycerol) was added to the worm pellets, which were then boiled for 15 minutes with occasional vortexing and then centrifuged to pellet the debris. The protein concentration of the lysates (supernatant from the previous step) was determined using the BCA Protein Assay Kit (Pierce, Thermo Fisher Scientific, Inc., Waltham, MA). For each sample, 50 μg of total protein was separated on a 10% SDS-PAGE gel (Bio-Rad Laboratories, Inc., Hercules, CA) and then transferred to a nitrocellulose membrane (GE Healthcare, Little Chalfont, United Kingdom). Blots were blocked with TBST supplemented with 5% skim milk power and then probed with either a 1:1,000 dilution of rabbit anti-ACTIVE p38 MAPK pAb (Promega Corp., Madison, WI), which recognizes the dually phosphorylated TGY motif of activated p38 MAPK, or a 1:10,000 dilution of mouse anti-β-tubulin (E7 Developmental Hybridoma Bank, Iowa City, IA) in TBST with 5% skim milk powder. Horseradish peroxidase (HRP)-conjugated anti-rabbit and anti-mouse IgG secondary antibodies (Cell Signaling Technology, Inc., Danvers, MA) were used followed by detection with ECL reagents (GE Healthcare).
RNA interference
Feeding RNAi was performed as previously described [44]. NGM agar plates supplemented with 2 mM isopropyl-β-D-thiogalactopyranoside (IPTG) and 25 μg/ml of carbenicillin were seeded with E. coli HT115 bacterial cultures carrying the control plasmid pPD129.36 (Ligation number L4440) or carrying specific plasmids derived from pPD129.36 designed to target either sek-1 or pmk-2 for RNAi. For pathogenesis assays and visualization of GFP expression from the agIs219 reporter, 3–6 L4 larval stage worms were placed onto seeded RNAi plates and their progeny were assayed. For Western analysis, ~10 L4 larval stage worms were placed onto seeded RNAi plates and the progeny of these worms were harvested before being deprived of food.
Plasmids
The pmk operon translational reporter was constructed through ligation of overlapping PCR amplicons by homologous recombination in Saccharomyces cerevisiae strain FY2 as previously described [45]. A ~20.3 kb region containing the pmk operon and ~4.4 kb of upstream sequence was amplified from fosmid 34bC01 in adjacent fragments with at least 50 bp of overlap between fragments. For the pmk operon translational reporter carrying mutations in the 3’UTR of pmk-2, 90 bp reverse complement primers containing the desired mutations were designed and used to amplify the 3’UTR of pmk-2. The gene encoding GFP was amplified from pPD95.75 (Addgene, Cambridge, MA). The gene encoding mCherry was amplified from pCFJ90 (Addgene). The PCR amplicons were transformed into S. cerevisiae strain FY2 along with destination vector pNP30 (gift of N. Paquin, pNP30 is a pRS426-derived plasmid compatible with MosSCI integration at locus ttTi5605 on LGII) digested with XhoI and AvrII (New England Biosciences, Inc., Ipswich, MA). Yeast DNA was extracted with phenol-chloroform and transformed into DH5-α electrocompetent cells (Protein Express, Inc., Cincinnati, OH). Plasmids were prepped using a Miniprep Kit (Qiagen N.V., Venlo, Netherlands) and their sequences were verified. Germline transformations were performed as described previously [38].
Microscopy
To visualize expression of GFP from the agIs219 reporter transgene, adult worms (16–24 hr post L4 larval stage at 20°C) were picked over to an unseeded NGM agar plate and immobilized with 50 mM sodium azide. Worms were viewed using a Stereo V12 fluorescence microscope (Zeiss) and pictures were taken with an AxioCam MRc camera. To visualize expression of GFP and mCherry from the pmk operon translational reporter, L4 larval stage transgenic animals were immobilized with 50 mM sodium azide and mounted on 2% agarose pads. Worms were viewed using a LSM510 confocal microscope (Zeiss). Stacks of confocal images were acquired and processed in Fiji to obtain maximum projections [36]. All images were prepared in Photoshop (Adobe Systems, Inc., San Jose, CA).
Supporting Information
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Štítky
Genetika Reprodukční medicínaČlánek vyšel v časopise
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