Increased and Imbalanced dNTP Pools Symmetrically Promote Both Leading and Lagging Strand Replication Infidelity
The building blocks of DNA, dNTPs, are vital to life, and thus their production is carefully controlled within each cell. Under certain conditions, such as cancer, infection, or drugs, the overall dNTP level or dNTP balance can change. Using yeast genetics we manipulated the dNTP pool balance in unicellular baker's yeast and analysed the effects upon fidelity of DNA replication. We also disrupted mismatch repair, an internal safety system that corrects replication errors and is mutated in many cancers. By sequencing DNA from yeast cells with these alterations we gain insights into the mechanisms of mutation formation that contribute to genome instability. We find that the leading and lagging strand replication fidelity is affected similarly by the dNTP pool imbalance and that the mismatch repair machinery corrects replication errors driven by a dNTP pool imbalance with highly variable efficiencies.
Published in the journal:
. PLoS Genet 10(12): e32767. doi:10.1371/journal.pgen.1004846
Category:
Research Article
doi:
https://doi.org/10.1371/journal.pgen.1004846
Summary
The building blocks of DNA, dNTPs, are vital to life, and thus their production is carefully controlled within each cell. Under certain conditions, such as cancer, infection, or drugs, the overall dNTP level or dNTP balance can change. Using yeast genetics we manipulated the dNTP pool balance in unicellular baker's yeast and analysed the effects upon fidelity of DNA replication. We also disrupted mismatch repair, an internal safety system that corrects replication errors and is mutated in many cancers. By sequencing DNA from yeast cells with these alterations we gain insights into the mechanisms of mutation formation that contribute to genome instability. We find that the leading and lagging strand replication fidelity is affected similarly by the dNTP pool imbalance and that the mismatch repair machinery corrects replication errors driven by a dNTP pool imbalance with highly variable efficiencies.
Introduction
The integrity of an organism's genome is vital to its continued survival, whether unicellular microbe or complex large mammal [1]. Therefore, there are highly conserved mechanisms involved in regulating and protecting genetic material both during and post DNA replication. One of the first safety systems for DNA replication is the stringent control of dNTP synthesis by ribonucleotide reductase (RNR), which maintains concentrations of the individual dNTPs at different levels [1], [2]. RNR catalyses the rate-limiting step in the production of all four dNTPs for the synthesis of nuclear and mitochondrial DNA [3], [4]. In yeast, RNR is a multi-subunit complex comprised of a large subunit, which exist as a homodimer of Rnr1 proteins or a heterodimer of Rnr1/Rnr3 proteins, and a small subunit comprised of Rnr2/Rnr4 proteins. The large subunits contain allosteric specificity sites that modulate enzyme activity and control the balance of the four dNTPs by influencing the specific ribonucleoside diphosphate reduction reaction within the catalytic sites [5]. A highly conserved loop of 13 amino acid residues (Loop 2) connects the allosteric specificity and catalytic sites and is crucial for the correct allosteric regulation of the enzyme [6], [7].
The DNA polymerase selectivity, proofreading and mismatch repair are subsequent safety systems that determine the fidelity of DNA replication. The DNA polymerase selectivity ensures insertion of the correct nucleotide during DNA synthesis. Although the major replicative polymerases alpha (Pol α), delta (Pol δ), and epsilon (Pol ε) are high fidelity enzymes, their accuracy is dependent upon the supply of dNTPs [8]. The second mechanism is proofreading in which errors are removed from primer termini during replication by a 3′–5′ exonuclease activity. Errors that escape proofreading can still be repaired post-replication, through the mismatch repair system (MMR) (reviewed in [9]). The major components of MMR are the homologs of the bacterial MutS proteins, a heterodimer of either Msh2-Msh6 or Msh2-Msh3 that recognise and bind to the mismatch. Msh2-Msh6 is mainly responsible for repairing single base-base mismatches, short insertions and deletions (indels) and small loops, whereas Msh2-Msh3 is involved in larger loop repair. Therefore, Msh2 is essential for MMR [10] and loss of this activity elevates mutation rates [11]. Mutation or loss of Msh2 in humans is associated with microsatellite instability and hereditary nonpolyposis colorectal cancer (HNPCC) [12] and gall bladder cancer [13].
The current model of the eukaryotic replication fork involves DNA polymerase complexes with very different subunit composition, enzymatic properties and fidelity. The leading strand is synthesized primarily by Pol ε, while the lagging strand is synthesized primarily by Pol α and Pol δ [14], [15]. Here we asked whether an imbalanced dNTP pool can force the leading and lagging strand polymerases to produce different errors. It is possible to answer this question by using a gene that is located close to an origin of replication and switching the leading and lagging strand synthesis. We previously created a panel of yeast strains with defined dNTP pool imbalances. The imbalances, in which none of dNTP levels was below normal, did not activate the genome integrity checkpoint and were highly mutagenic despite the availability of functional proofreading and MMR [16]. Utilizing a strain with elevated dTTP and dCTP concentrations and normal dATP and dGTP concentrations, we previously determined the rate and specificity of replication errors generated at the CAN1 locus [17]. As the CAN1 reporter gene is located close to a replication origin, by reversing the orientation of CAN1 and thereby switching the leading and lagging strand synthesis at this locus, we can analyse potential mutational strand bias. To determine the efficiency of DNA mismatch repair in the presence of this dNTP pool imbalance, we also created an rnr1-Y285A mutant strain that was MMR deficient. Our data demonstrate that the mutational potential of this dNTP pool imbalance overpowers the intrinsic differences in error specificity of the leading and lagging strand polymerases and reveals that MMR works with highly variable efficiency.
Results
dNTP pools of the rnr1-Y285A and msh2Δ strains
To examine potential differences in mutational specificity between the major replicative polymerases in the presence of the imbalanced dNTP pools, we reversed the orientation of the CAN1 gene (CAN1-OR2). To investigate the effect of this dNTP pool imbalance in the absence of MMR, we deleted MSH2 in the rnr1-Y285A CAN-OR1 strain. The msh2Δ single mutant strain had normal dNTP pools (Fig. 1). The dNTP pools in the double mutant had the same imbalance as in the single rnr1-Y285A [17], with approximately 26- and 14-fold higher concentrations of dCTP and dTTP, respectively, compared to wild type (wt) whilst the concentration of dATP was about double and dGTP was normal (Fig. 1).
CAN1 spontaneous mutation rates and types
The spontaneous CAN1 mutation rate in the rnr1-Y285A CAN1-OR2 was 13-fold higher than wt (Table 1), which was similar to the CAN1-OR1 previously published OR1 [17]. The msh2Δ mutant had a 15-fold higher mutation rate compared to wt, however, the rnr1-Y285A msh2Δ strain mutation rate was over 500-fold greater than that of wt and over 30-fold either of the relative single mutants. Indels were the major mutation type observed in all four mutant strains whereas it was single base substitutions in wt (Fig. 2). The rnr1-Y285A, with CAN1 in both orientations, and msh2Δ strains had an average increase in the indel rate of more than 60-fold the wt strain (0.5×107 for wt versus 33×107 for OR1 [17], 37× for OR2, and 42×107 for msh2Δ). However, the double mutant indel rate was increased the most at more than 2000-fold over wt. In addition to single base indels, base substitutions were also significantly increased in the mutants to over 8-fold higher in the single mutants and 350-fold higher in rnr1-Y285A msh2Δ compared to wt. Complex mutations, defined as mutations involving insertions or deletions of multiple bases, were much more common in the double mutant, occurring at a rate over 30 times higher than that in wild type.
Mutation hotspots
Replication of the CAN1 gene originates from ARS507 and travels through the gene towards the telomere [18]–[20] (Fig. 3A). Therefore, in rnr1-Y285A CAN1-OR1 the leading strand polymerase, presumed to be Pol ε [21], uses the non-coding strand as the template while the coding strand is the template for lagging strand replication primarily by Pol δ or Pol α [14]. In CAN1-OR2, Pol ε now copies the coding strand and Pol δ/Pol α copy the non-coding strand (see Fig. 3A). An example is given in Fig. 3B, for the single base substitution at 648 bp. During leading strand synthesis in OR1, no mistake is made when copying template G due to high concentration of dCTP. However, during lagging strand replication dTTP is inserted opposite template C, as dTTP is at a much higher concentration than the dGTP required for correct pairing. As the succeeding incoming nucleotides are also at an increased concentration, rapid extension then follows stabilizing the C: dT mismatch. In the next round of replication, a C to A mutation arises. When the gene is reversed in rnr1-Y285A CAN1-OR2, Pol ε now copies the template C with low fidelity by misinserting dTTP, which ultimately results in a C to A mutation, and Pol δ/Pol α replication is error-free.
Hotspots, mutation sites where the rates were ≥10-fold greater than wt, were assigned to have occurred during leading or lagging strand synthesis by the nature of the mutation observed and dNTP pool imbalance. Simplified mutational spectra illustrating the hotspots in the CAN1 gene for each strain are shown in Fig. 4 with the full spectra in Figure S1-S3. The hotspot mutation rate was calculated by the equation [(frequency/total no. of samples) x CAN1 mutation rate]. The majority of hotspots in rnr1-Y285A CAN1-OR1 and OR2 show no leading – lagging strand bias and have similar mutation rates in both strains (Fig. 5). However, the major hotspot at position 425–427 bp was only seen in OR2 and had a mutation rate of 48×10−8 which was 15-fold higher than in OR1.
The rnr1-Y285A and msh2Δ strains had several shared major hotspots. Three single base deletions occurred in G: C homopolymeric runs at 757–760 bp, 795–797 bp, and 857–859 bp and two single base substitutions at 313 bp and 1379 bp (Fig. 4 and Table S1). Whilst the double mutant shared these five hotspots with both single mutants, there was a more than 100-fold increase in rates for base substitutions at 313 bp and 13791 bp (Fig. 6A and Table S1). The major hotspots in rnr1-Y285A msh2Δ were those seen in msh2Δ at 1118–1121 bp, 1392 bp, and especially the dominant deletion hotspots at 620–625 bp, 964–969 bp, and 1381–1386 bp. The site-specific mutation rates in the double mutant ranged from 4- to 800-fold the single mutants.
MMR efficiency
Analysis of the mutation spectra in the rnr1-Y285A and rnr1-Y285A msh2Δ strains showed that MMR had different efficiency at distinct mismatches. The ratio of mutation rates in the msh2Δ and MMR-proficient strains gave site-specific MMR correction efficiency (Fig. 6B and Table S1). The five hotspots (314, 718, 757, 795 and 857 bp), which include those with the highest mutation rates in the single rnr1-Y285A mutant, were the same sites that MMR was the least efficient at repairing errors. The correction factors were less than 30 and only around 10 in four of these sites (i.e., around 10% of errors at these sites will remain uncorrected by MMR). The majority of sites with the highest mutation rates in the double mutant (620, 964, 1118, 1381, and 1392 bp) were those that MMR was best at repairing, namely at T: A mononucleotide repeats. Loss of MSH2 increased the mutation rate at these sites by 260- to 800-fold.
Discussion
Increased dCTP and dTTP drive different polymerases to make similar errors
Despite the inherent differences in complexity of continuous (leading strand) and non-continuous (lagging strand) synthesis, the increased dCTP and dTTP drive the same kind of mutations at identical sequences regardless of the replicative DNA polymerase. Most of the mutations occurred at a G: C base pair in which the cytosine served as the template for synthesis and was often flanked by a 5′-A or a tract of purines as exemplified in Fig. 3. With the concentration of dGTP being the lowest and dCTP and dTTP the highest, the deletion of a G: C base pair in a mononucleotide repeat is stabilized by the rapid incorporation of the next incoming nucleotide (dTTP opposite the template A), as described in detail in our previous report [17]. This dNTP imbalance and sequence context also explains the G: C to T: A base substitutions where dTTP is misinserted opposite template C and mismatch extension proceeds with the rapid incorporation of the pyrimidines opposite the flanking tract of purines. Thus, the mismatch remains at the expense of polymerase proofreading.
However, an exception was found at 425 bp (where there is a hotspot found only when the CAN1 gene is reversed to OR2). Although similar sequences (AT runs) show no variation in mutation rates between orientations it appears that polymerase δ or α could be making a mistake at this point but not Pol ε. There were also several minor hotspots that suggest polymerase specificity (positions 538, 937, and 971 were unique to OR1 whereas 387 and 1353 were seen only in OR2) which could be indicative of the differences in polymerase efficiency in certain sequence contexts. Whole genome sequencing may give insight into other sites and contexts that affect polymerase specificity and establish the patterns of mutations arising in the presence of this dNTP pool imbalance.
Given that the concentration of dATP was also lower than dCTP and dTTP, T: A to G: C transversions could also be expected in the base substitution hotspots where dCTP is misinserted opposite template T during replication. The nucleotide ratio of dCTP: dATP increased from ∼1∶1 in the wt strain to ∼6∶1 in the rnr1-Y285A strains. However, the increase in the nucleotide ratio of dTTP: dGTP was larger, from ∼4∶1 in the wt strain to ∼38∶1 in the rnr1-Y285A strains, which may explain the prevalence of the G: C to T: A transversions. Furthermore, the lack of T: A to G: C transversions may be due to the intrinsic difference in the rates at which the two errors are generated. Recent genome-wide studies in S. cerevisiae have reported that G: C to T: A transversions were observed at a higher rate than T: A to G: C in strains with normal dNTP pools [22], [23]. The three major replicative polymerases were more prone to generate G: C to T: A transversions but very rarely generated T: A to G: C transversions [23]. In addition, tumours with somatic mutations in the exonuclease domain of Pol ε have a higher prevalence of C to A mutations [24]–[28].
MMR repairs replication errors driven by a dNTP pool imbalance with highly variable efficiencies
MMR efficiency was dependent upon the site and mismatch generated from the dNTP pool imbalance. The increase in indels in the msh2Δ strains was not surprising as MMR is known to be highly active at repairing mistakes at mononucleotide repeats [29], [30]. The indels were almost entirely unique to sequences with ≥3 mononucleotide repeats in the double mutant (99.2%, 127 of 128) compared to 91% in the msh2Δ mutant and most frequently occurred in A: T runs. This can be predicted as A-T mononucleotide repeats are often the site of indels in MMR deficient strains [31] and are by far the most common in the CAN1 gene sequence (Figure S4). Indeed, it appears that the relationship between mutation rate and mononucleotide repeat length is exponential as others have found across the whole yeast genome [22].
The MMR correction factor for all indels in the rnr1-Y285A background was 32, which means that on average, 31 of 32 indels are corrected by MMR (compare Fig. 2 rates). Nevertheless, this is ∼3-fold lower than that in the wt RNR background suggesting that some indels driven by this dNTP pool imbalance escape MMR. In addition, there were several major indel hotspots, mainly at G: C base pairs in mononucleotide repeats, with correction factors of 10 compared to the indels at A: T repeats which ranged from 200 to 800. This is a huge variation in the vital post-replication repair machinery that supports the notion of MMR efficiency being dependent on the dNTP pool imbalance, sequence context, and identity of the mismatch.
There are several possibilities as to why MMR is not efficient at these sites in the rnr1-Y285A strain. First, there could be a saturation of MMR due to the volume of errors induced by the pool imbalance that are not corrected by proofreading [32]. Consider the hotspots at 795 and 857 bp which dominated the spectra in rnr1-Y285A. The correction factor for the wt RNR strain was more than 2- and 5-fold higher than for the rnr1-Y285A mutant at the 795 and 857 hotspots, respectively (S1 Table). Therefore, MMR was more accurate at repairing deletions at these sites in the wt RNR strain with normal dNTP pools. Second, MMR itself may require a natural dNTP pool balance in order to correctly repair mistakes. If MMR complexes recognise the mismatches generated but recruit an error-prone or even high fidelity polymerase, the imbalanced dNTP concentration may result in the same mismatch; thus, the mutation is maintained. Finally, some mismatches may not be subjected to MMR if they are damaged or generated outside of DNA replication [33]–[37].
Materials and Methods
Yeast strains
The CAN1 gene was replaced with URA3 from the pUG72 plasmid [38] (primers “CAN1 Del Ura3” in Materials and Methods S1) in the RNR mutated strain (rnr1-Y285A) previously described [16]. PCR amplified WT CAN1 in reversed orientation (primers “CAN1 orientation” in Materials and Methods S1) was then transformed into the can1:: URA3 strain to give rnr1-Y285A CAN1 OR2. 5-FOA selection allowed the elimination of any can1:: URA3 cells and the CAN1 reverse orientation was confirmed by sequencing (“can1ori scr” primers in Materials and Methods S1).
An MMR deficient strain was created by deleting MSH2 in the AC402 wt (to give msh2Δ) using the pAG32 plasmid and transfection technique [39] and the primers msh2_hphMX4, shown in Materials and Methods S1. The deletion was confirmed using primers flanking MSH2. This strain was then crossed with rnr1-Y285A [16], sporulated and dissected spores selected on Hygromycin and –Trp plates for the double mutant rnr1-Y285A msh2Δ.
Culture conditions and Canavanine resistance assay
All culturing was carried out at 30°C in YPAD (1% yeast extract, 2% bacto-peptone, 20 mg/l adenine, 2% agar for plates) liquid cultures in a shaking incubator at 160rpm. The Canavanine resistance assay was used to calculate mutation rates as previously described [17], [40], [41]. The Canr colonies were picked and the CAN1 gene amplified and sequenced (MWG Eurofins) using published primers [17] to produce the mutation spectra.
dNTP pool measurements
dNTP pools were measured in asynchronous cultures as described previously [16] with minor changes as described in [42]. Briefly, cells were harvested by filtration at a density of 0.4×107 to 0.5×107 cells/ml and NTPs and dNTPs were extracted in trichloroacetic acid and MgCl2 followed by a Freon-trioctylamine mix. dNTPs were separated using boronate columns (Affigel 601, Biorad) and analysed by HPLC on a LaChrom Elite UV detector (Hitachi) with a Partisphere SAX column (Hichrom, UK).
Supporting Information
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Genetika Reprodukční medicínaČlánek vyšel v časopise
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