Molecular genetics of maternally-controlled cell divisions
Autoři:
Elliott W. Abrams aff001; Ricardo Fuentes aff001; Florence L. Marlow aff001; Manami Kobayashi aff001; Hong Zhang aff001; Sumei Lu aff001; Lee Kapp aff001; Shai R. Joseph aff003; Amy Kugath aff001; Tripti Gupta aff001; Virginia Lemon aff001; Greg Runke aff001; Amanda A. Amodeo aff004; Nadine L. Vastenhouw aff003; Mary C. Mullins aff001
Působiště autorů:
Department of Cell and Developmental Biology, University of Pennsylvania Perelman School of Medicine, Philadelphia, Pennsylvania, United States of America
aff001; Department of Biology, Purchase College, The State University of New York, Purchase, New York, United States of America
aff002; Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany
aff003; Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, New Jersey, United States of America
aff004
Vyšlo v časopise:
Molecular genetics of maternally-controlled cell divisions. PLoS Genet 16(4): e1008652. doi:10.1371/journal.pgen.1008652
Kategorie:
Research Article
doi:
https://doi.org/10.1371/journal.pgen.1008652
Souhrn
Forward genetic screens remain at the forefront of biology as an unbiased approach for discovering and elucidating gene function at the organismal and molecular level. Past mutagenesis screens targeting maternal-effect genes identified a broad spectrum of phenotypes ranging from defects in oocyte development to embryonic patterning. However, earlier vertebrate screens did not reach saturation, anticipated classes of phenotypes were not uncovered, and technological limitations made it difficult to pinpoint the causal gene. In this study, we performed a chemically-induced maternal-effect mutagenesis screen in zebrafish and identified eight distinct mutants specifically affecting the cleavage stage of development and one cleavage stage mutant that is also male sterile. The cleavage-stage phenotypes fell into three separate classes: developmental arrest proximal to the mid blastula transition (MBT), irregular cleavage, and cytokinesis mutants. We mapped each mutation to narrow genetic intervals and determined the molecular basis for two of the developmental arrest mutants, and a mutation causing male sterility and a maternal-effect mutant phenotype. One developmental arrest mutant gene encodes a maternal specific Stem Loop Binding Protein, which is required to maintain maternal histone levels. The other developmental arrest mutant encodes a maternal-specific subunit of the Minichromosome Maintenance Protein Complex, which is essential for maintaining normal chromosome integrity in the early blastomeres. Finally, we identify a hypomorphic allele of Polo-like kinase-1 (Plk-1), which results in a male sterile and maternal-effect phenotype. Collectively, these mutants expand our molecular-genetic understanding of the maternal regulation of early embryonic development in vertebrates.
Klíčová slova:
Cell cycle and cell division – DNA-binding proteins – Embryos – Histones – Chromosome mapping – Messenger RNA – Phenotypes – Zebrafish
Zdroje
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