O-linked β-N-acetylglucosamine transferase plays an essential role in heart development through regulating angiopoietin-1
Autoři:
Yongxin Mu aff001; Houzhi Yu aff001; Tongbin Wu aff001; Jianlin Zhang aff001; Sylvia M. Evans aff001; Ju Chen aff001
Působiště autorů:
Department of Medicine-Cardiology, University of California San Diego,Gilman Drive, Mail Code, La Jolla, California, United States of America
aff001; Department of Cardiology, Shandong Provincial Hospital affiliated to Shandong University, Jinan, China
aff002; Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California at San Diego, La Jolla, CA, United States of America
aff003
Vyšlo v časopise:
O-linked β-N-acetylglucosamine transferase plays an essential role in heart development through regulating angiopoietin-1. PLoS Genet 16(4): e32767. doi:10.1371/journal.pgen.1008730
Kategorie:
Research Article
doi:
https://doi.org/10.1371/journal.pgen.1008730
Souhrn
O-linked N-acetylglucosamine (GlcNAc) transferase (OGT) is the only enzyme catalyzing O-GlcNAcylation. Although it has been shown that OGT plays an essential role in maintaining postnatal heart function, its role in heart development remains unknown. Here we showed that loss of OGT in early fetal cardiomyocytes led to multiple heart developmental defects including hypertrabeculation, biventricular dilation, atrial septal defects, ventricular septal defects, and defects in coronary vessel development. In addition, RNA sequencing revealed that Angiopoietin-1, required within cardiomyocytes for both myocardial and coronary vessel development, was dramatically downregulated in cardiomyocyte-specific OGT knockout mouse hearts. In conclusion, our data demonstrated that OGT plays an essential role in regulating heart development through activating expression of cardiomyocyte Angiopoietin-1.
Klíčová slova:
Cardiomyocytes – Coronary arteries – Embryos – Heart – Heart development – Immunostaining – Mouse models – Ventricular septal defects
Zdroje
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