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Runx2 is essential for the transdifferentiation of chondrocytes into osteoblasts


Autoři: Xin Qin aff001;  Qing Jiang aff001;  Kenichi Nagano aff002;  Takeshi Moriishi aff003;  Toshihiro Miyazaki aff003;  Hisato Komori aff001;  Kosei Ito aff004;  Klaus von der Mark aff005;  Chiharu Sakane aff006;  Hitomi Kaneko aff001;  Toshihisa Komori aff001
Působiště autorů: Basic and Translational Research Center for Hard Tissue Disease, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan aff001;  Department of Oral Pathology and Bone Metabolism, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan aff002;  Department of Cell Biology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan aff003;  Department of Molecular Bone Biology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan aff004;  Department of Experimental Medicine I, Nikolaus-Fiebiger Center of Molecular Medicine, Friedrich-Alexander University Erlangen-Nürnberg, Erlangen, Germany aff005;  Division of Comparative Medicine, Life Science Support Center, Nagasaki University, Nagasaki, Japan aff006
Vyšlo v časopise: Runx2 is essential for the transdifferentiation of chondrocytes into osteoblasts. PLoS Genet 16(11): e1009169. doi:10.1371/journal.pgen.1009169
Kategorie: Research Article
doi: https://doi.org/10.1371/journal.pgen.1009169

Souhrn

Chondrocytes proliferate and mature into hypertrophic chondrocytes. Vascular invasion into the cartilage occurs in the terminal hypertrophic chondrocyte layer, and terminal hypertrophic chondrocytes die by apoptosis or transdifferentiate into osteoblasts. Runx2 is essential for osteoblast differentiation and chondrocyte maturation. Runx2-deficient mice are composed of cartilaginous skeletons and lack the vascular invasion into the cartilage. However, the requirement of Runx2 in the vascular invasion into the cartilage, mechanism of chondrocyte transdifferentiation to osteoblasts, and its significance in bone development remain to be elucidated. To investigate these points, we generated Runx2fl/flCre mice, in which Runx2 was deleted in hypertrophic chondrocytes using Col10a1 Cre. Vascular invasion into the cartilage was similarly observed in Runx2fl/fl and Runx2fl/flCre mice. Vegfa expression was reduced in the terminal hypertrophic chondrocytes in Runx2fl/flCre mice, but Vegfa was strongly expressed in osteoblasts in the bone collar, suggesting that Vegfa expression in bone collar osteoblasts is sufficient for vascular invasion into the cartilage. The apoptosis of terminal hypertrophic chondrocytes was increased and their transdifferentiation was interrupted in Runx2fl/flCre mice, leading to lack of primary spongiosa and osteoblasts in the region at E16.5. The osteoblasts appeared in this region at E17.5 in the absence of transdifferentiation, and the number of osteoblasts and the formation of primary spongiosa, but not secondary spongiosa, reached to levels similar those in Runx2fl/fl mice at birth. The bone structure and volume and all bone histomophometric parameters were similar between Runx2fl/fl and Runx2fl/flCre mice after 6 weeks of age. These findings indicate that Runx2 expression in terminal hypertrophic chondrocytes is not required for vascular invasion into the cartilage, but is for their survival and transdifferentiation into osteoblasts, and that the transdifferentiation is necessary for trabecular bone formation in embryonic and neonatal stages, but not for acquiring normal bone structure and volume in young and adult mice.

Klíčová slova:

Cartilage – Bone development – Femur – Chondrocytes – Ossification – Osteoblasts – Safranin staining – Transdifferentiation


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