Transcriptional and genomic parallels between the monoxenous parasite Herpetomonas muscarum and Leishmania

English version

Autoři: Megan A. Sloan ^aff001; Karen Brooks ^aff002; Thomas D. Otto ^aff002; Mandy J. Sanders ^aff002; James A. Cotton ^aff002; Petros Ligoxygakis ^aff001
Působiště autorů: Department of Biochemistry, University of Oxford, Oxford, United Kingdom ^aff001; The Wellcome Sanger Institute, Wellcome Genome Campus, Hixton, Cambridgeshire, United Kingdom ^aff002
Vyšlo v časopise: Transcriptional and genomic parallels between the monoxenous parasite Herpetomonas muscarum and Leishmania. PLoS Genet 15(11): e32767. doi:10.1371/journal.pgen.1008452
Kategorie: Research Article
doi: https://doi.org/10.1371/journal.pgen.1008452

Souhrn

Trypanosomatid parasites are causative agents of important human and animal diseases such as sleeping sickness and leishmaniasis. Most trypanosomatids are transmitted to their mammalian hosts by insects, often belonging to Diptera (or true flies). These are called dixenous trypanosomatids since they infect two different hosts, in contrast to those that infect just insects (monoxenous). However, it is still unclear whether dixenous and monoxenous trypanosomatids interact similarly with their insect host, as fly-monoxenous trypanosomatid interaction systems are rarely reported and under-studied–despite being common in nature. Here we present the genome of monoxenous trypanosomatid Herpetomonas muscarum and discuss its transcriptome during in vitro culture and during infection of its natural insect host Drosophila melanogaster. The H. muscarum genome is broadly syntenic with that of human parasite Leishmania major. We also found strong similarities between the H. muscarum transcriptome during fruit fly infection, and those of Leishmania during sand fly infections. Overall this suggests Drosophila-Herpetomonas is a suitable model for less accessible insect-trypanosomatid host-parasite systems such as sand fly-Leishmania.

Klíčová slova:

Drosophila melanogaster – Ingestion – Invertebrate genomics – Leishmania – Protein domains – Transcriptome analysis – Trypanosoma – Trypanosoma brucei gambiense

Zdroje

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