A circadian output center controlling feeding:Fasting rhythms in Drosophila

English version

Autoři: Austin P. Dreyer ^aff001; Madison M. Martin ^aff001; Carson V. Fulgham ^aff001; Daniel A. Jabr ^aff001; Lei Bai ^aff002; Jennifer Beshel ^aff001; Daniel J. Cavanaugh ^aff001
Působiště autorů: Department of Biology, Loyola University Chicago, Chicago, Illinois, United States of America ^aff001; Penn Chronobiology, Howard Hughes Medical Institute, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, United States of America ^aff002
Vyšlo v časopise: A circadian output center controlling feeding:Fasting rhythms in Drosophila. PLoS Genet 15(11): e1008478. doi:10.1371/journal.pgen.1008478
Kategorie: Research Article
doi: https://doi.org/10.1371/journal.pgen.1008478

Souhrn

Circadian rhythms allow animals to coordinate behavioral and physiological processes with respect to one another and to synchronize these processes to external environmental cycles. In most animals, circadian rhythms are produced by core clock neurons in the brain that generate and transmit time-of-day signals to downstream tissues, driving overt rhythms. The neuronal pathways controlling clock outputs, however, are not well understood. Furthermore, it is unclear how the central clock modulates multiple distinct circadian outputs. Identifying the cellular components and neuronal circuitry underlying circadian regulation is increasingly recognized as a critical step in the effort to address health pathologies linked to circadian disruption, including heart disease and metabolic disorders. Here, building on the conserved components of circadian and metabolic systems in mammals and Drosophila melanogaster, we used a recently developed feeding monitor to characterize the contribution to circadian feeding rhythms of two key neuronal populations in the Drosophila pars intercerebralis (PI), which is functionally homologous to the mammalian hypothalamus. We demonstrate that thermogenetic manipulations of PI neurons expressing the neuropeptide SIFamide (SIFa) as well as mutations of the SIFa gene degrade feeding:fasting rhythms. In contrast, manipulations of a nearby population of PI neurons that express the Drosophila insulin-like peptides (DILPs) affect total food consumption but leave feeding rhythms intact. The distinct contribution of these two PI cell populations to feeding is accompanied by vastly different neuronal connectivity as determined by trans-Tango synaptic mapping. These results for the first time identify a non-clock cell neuronal population in Drosophila that regulates feeding rhythms and furthermore demonstrate dissociable control of circadian and homeostatic aspects of feeding regulation by molecularly-defined neurons in a putative circadian output hub.

Klíčová slova:

Biological locomotion – Circadian oscillators – Circadian rhythms – Drosophila melanogaster – Food consumption – Chronobiology – Neurons – Cell activation

Zdroje

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