SON protects nascent transcripts from unproductive degradation by counteracting DIP1

English version

Autoři: Mandy Li-Ian Tay ^aff001; Jun Wei Pek ^aff001
Působiště autorů: Temasek Life Sciences Laboratory, Singapore, Singapore ^aff001; Department of Biological Sciences, National University of Singapore, Singapore ^aff002
Vyšlo v časopise: SON protects nascent transcripts from unproductive degradation by counteracting DIP1. PLoS Genet 15(11): e32767. doi:10.1371/journal.pgen.1008498
Kategorie: Research Article
doi: https://doi.org/10.1371/journal.pgen.1008498

Souhrn

Gene expression involves the transcription and splicing of nascent transcripts through the removal of introns. In Drosophila, a double-stranded RNA binding protein Disco-interacting protein 1 (DIP1) targets INE-1 stable intronic sequence RNAs (sisRNAs) for degradation after splicing. How nascent transcripts that also contain INE-1 sequences escape degradation remains unknown. Here we observe that these nascent transcripts can also be bound by DIP1 but the Drosophila homolog of SON (Dsn) protects them from unproductive degradation in ovaries. Dsn localizes to the satellite body where active decay of INE-1 sisRNAs by DIP1 occurs. Dsn is a repressor of DIP1 posttranslational modifications (primarily sumoylation) that are assumed to be required for efficient DIP1 activity. Moreover, the pre-mRNA destabilization caused by Dsn depletion is rescued in DIP1 or Sumo heterozygous mutants, suggesting that Dsn is a negative regulator of DIP1. Our results reveal that under normal circumstances nascent transcripts are susceptible to DIP1-mediated degradation, however intronic sequences are protected by Dsn until intron excision has taken place.

Klíčová slova:

Drosophila melanogaster – Gene expression – Immunoprecipitation – Introns – Messenger RNA – Ovaries – RNA interference – SUMOylation

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