Muc2 Protects against Lethal Infectious Colitis by Disassociating Pathogenic and Commensal Bacteria from the Colonic Mucosa
Despite recent advances in our understanding of the pathogenesis of attaching and effacing (A/E) Escherichia coli infections, the mechanisms by which the host defends against these microbes are unclear. The goal of this study was to determine the role of goblet cell-derived Muc2, the major intestinal secretory mucin and primary component of the mucus layer, in host protection against A/E pathogens. To assess the role of Muc2 during A/E bacterial infections, we inoculated Muc2 deficient (Muc2−/−) mice with Citrobacter rodentium, a murine A/E pathogen related to diarrheagenic A/E E. coli. Unlike wildtype (WT) mice, infected Muc2−/− mice exhibited rapid weight loss and suffered up to 90% mortality. Stool plating demonstrated 10–100 fold greater C. rodentium burdens in Muc2−/− vs. WT mice, most of which were found to be loosely adherent to the colonic mucosa. Histology of Muc2−/− mice revealed ulceration in the colon amid focal bacterial microcolonies. Metabolic labeling of secreted mucins in the large intestine demonstrated that mucin secretion was markedly increased in WT mice during infection compared to uninfected controls, suggesting that the host uses increased mucin release to flush pathogens from the mucosal surface. Muc2 also impacted host-commensal interactions during infection, as FISH analysis revealed C. rodentium microcolonies contained numerous commensal microbes, which was not observed in WT mice. Orally administered FITC-Dextran and FISH staining showed significantly worsened intestinal barrier disruption in Muc2−/− vs. WT mice, with overt pathogen and commensal translocation into the Muc2−/− colonic mucosa. Interestingly, commensal depletion enhanced C. rodentium colonization of Muc2−/− mice, although colonic pathology was not significantly altered. In conclusion, Muc2 production is critical for host protection during A/E bacterial infections, by limiting overall pathogen and commensal numbers associated with the colonic mucosal surface. Such actions limit tissue damage and translocation of pathogenic and commensal bacteria across the epithelium.
Published in the journal:
. PLoS Pathog 6(5): e32767. doi:10.1371/journal.ppat.1000902
Category:
Research Article
doi:
https://doi.org/10.1371/journal.ppat.1000902
Summary
Despite recent advances in our understanding of the pathogenesis of attaching and effacing (A/E) Escherichia coli infections, the mechanisms by which the host defends against these microbes are unclear. The goal of this study was to determine the role of goblet cell-derived Muc2, the major intestinal secretory mucin and primary component of the mucus layer, in host protection against A/E pathogens. To assess the role of Muc2 during A/E bacterial infections, we inoculated Muc2 deficient (Muc2−/−) mice with Citrobacter rodentium, a murine A/E pathogen related to diarrheagenic A/E E. coli. Unlike wildtype (WT) mice, infected Muc2−/− mice exhibited rapid weight loss and suffered up to 90% mortality. Stool plating demonstrated 10–100 fold greater C. rodentium burdens in Muc2−/− vs. WT mice, most of which were found to be loosely adherent to the colonic mucosa. Histology of Muc2−/− mice revealed ulceration in the colon amid focal bacterial microcolonies. Metabolic labeling of secreted mucins in the large intestine demonstrated that mucin secretion was markedly increased in WT mice during infection compared to uninfected controls, suggesting that the host uses increased mucin release to flush pathogens from the mucosal surface. Muc2 also impacted host-commensal interactions during infection, as FISH analysis revealed C. rodentium microcolonies contained numerous commensal microbes, which was not observed in WT mice. Orally administered FITC-Dextran and FISH staining showed significantly worsened intestinal barrier disruption in Muc2−/− vs. WT mice, with overt pathogen and commensal translocation into the Muc2−/− colonic mucosa. Interestingly, commensal depletion enhanced C. rodentium colonization of Muc2−/− mice, although colonic pathology was not significantly altered. In conclusion, Muc2 production is critical for host protection during A/E bacterial infections, by limiting overall pathogen and commensal numbers associated with the colonic mucosal surface. Such actions limit tissue damage and translocation of pathogenic and commensal bacteria across the epithelium.
Introduction
The attaching and effacing (A/E) bacteria Enteropathogenic Escherichia coli (EPEC) and Enterohemorrhagic E. coli (EHEC) are major contributors to the global disease burden caused by enteric bacterial pathogens [1]. EPEC infects the small bowel causing acute watery diarrhea, fever and nausea [1], [2] and is an important cause of infant diarrheal disease in developing countries. EPEC infections lead to the deaths of hundreds of thousands of infants annually from dehydration and other complications [1], [3]. In contrast, EHEC (O157:H7) infection is associated with sporadic outbreaks across industrialized countries, due to consumption of contaminated beef or water supplies [1], [4]. EHEC colonizes the large bowel and secretes the highly cytotoxic Shiga Toxin (Stx), which can lead to severe hemorrhagic colitis and bloody diarrhea in people of all ages [5]. Children are at an additional risk of EHEC-induced Hemolytic Uremic Syndrome, a potentially fatal complication caused by Stx-mediated acute renal failure [6]. Both EPEC and EHEC are minimally invasive, as they intimately attach to the apical plasma membrane of intestinal epithelial cells via a Type 3 Secretion System (T3SS). Infection causes localized destruction (effacement) of the epithelial microvilli to form the unique A/E lesion [7]. Significant advances have been made in delineating the mechanisms of A/E lesion formation and their requirement for disease [8]; however, the factors involved in host susceptibility to and defense against A/E pathogens remain ill defined.
As EPEC and EHEC are human-specific and do not cause relevant disease in animal models [7], our understanding of innate and adaptive immunity against these pathogens has come from studying related A/E bacteria that infect other mammals. Citrobacter rodentium is a natural A/E pathogen of mice that infects epithelial cells lining the cecum, descending colon and rectum of the murine large bowel [7], [9]. C. rodentium infection leads to an acute colitis, mucosal hyperplasia, barrier disruption, and loose stools, but is resolved in 3–4 weeks in C57BL/6 mice [10]. Since C. rodentium uses similar virulence strategies to those employed by EPEC and EHEC to infect cells, including T3SS-mediated intimate attachment and A/E lesion formation, it is widely used as an in vivo model of A/E bacterial infection [10]. The C. rodentium model also allows for identification of the cells and mediators utilized by the host to control infections by A/E pathogens. While a robust adaptive immune response involving CD4+ T cells and B cells (via immunoglobulin G (IgG) secretion) is required for pathogen clearance [11], [12], studies have shown epithelial cells to be important in limiting C. rodentium colonization [13], [14]. In this regard, mounting evidence suggests epithelial-derived mucin production is an additional defense mechanism to manage enteric bacterial infections [15], [16]. Mucins are high molecular weight glycoproteins characterized by extended serine, threonine, and proline-rich domains in the protein core, which are sites of extensive O-linked glycosylation with oligosaccharides [17]. The mucin gene family contains 16 known members in humans that can be broadly divided into membrane bound or secretory forms [15]. The membrane-bound Muc1, which is produced by all intestinal epithelial cells, has been shown to play a role in host defense against Campylobacter jejuni in vivo, limiting disease and systemic spread [18]. Muc1 is also upregulated in C. rodentium infection [19], although its role in this infection is not known. However, membrane-bound MUC3 has been associated with decreased colonization of EPEC in vitro [20]. Collectively, these studies suggest that mucins may play a role in limiting the pathogenesis of A/E infections.
MUC2 (mouse, Muc2) is the major colonic secretory mucin in humans and mice [21], [22]. In contrast to other epithelial mucins in the gut, MUC2 is synthesized specifically by goblet cells of the small and large intestine [22]. These cells constitutively produce MUC2 polymers, which are densely packaged into numerous apically-stored granules, and released into the intestinal lumen to form the structural basis of the mucus–gel layer [21], [23]. This layer is a biochemically complex medium, rich in carbohydrates, antimicrobial peptides and other proteins, as well as lipids and electrolytes [23], [24]. The depth of the mucus layer varies with the region of the intestinal tract, but is thickest in the colon and rectum, reaching over 800 µm in rodents [25]. Studies have revealed that Muc2-mediated mucus formation in the mammalian colon leads to 2 distinct sublayers; an inner layer that is firmly adherent to the intestinal mucosa, and an outer layer that can be washed off with minimal rinsing [26], [27]. Interestingly, commensal bacteria heavily colonize the outer of these two layers, whereas the inner layer is virtually sterile [27]. The mechanisms underlying the formation and function of these sublayers is still under investigation; however, studies in animal models have indicated that Muc2-dependent mucus production profoundly impacts intestinal physiology, as demonstrated in vivo with the generation of Muc2 deficient (Muc2−/−) mice [28], which lack a mucus layer [27]. Depending on their genetic background, aged Muc2−/− mice may develop colorectal cancer [28] and/or spontaneous colitis [29]. Although the exact mechanisms that lead to these intestinal disorders are still elusive, deficiency in mucus production appears to alter the normal localization of commensal microbiota within the colon [27] as well as disrupt the mechanisms that govern epithelial [28], [30], [31] and immune homeostasis [29], [32].
Despite the role of Muc2 in regulating commensal and gut homeostasis, its role in host defense against epithelial-adherent pathogens such as A/E bacteria is not clear. In vitro studies have implicated MUC2 in limiting colonization of epithelial cells by EPEC [20], however the biological significance of this in vivo is undetermined. Indeed, considering that A/E pathogens colonize the mucosal surface and should therefore be constantly in contact with secreted Muc2, there is surprisingly little known about how these pathogens interact with Muc2 and the mucus layer in vivo. This is a critical question since the Muc2-dependent mucus layer is one of the first anatomical features bacteria such as A/E pathogens must encounter before reaching the intestinal epithelium [33]. Such early interactions could therefore profoundly influence the course of infection. The aim of our study was to use the C. rodentium model of A/E bacterial infection in Muc2-sufficient (wildtype) mice and Muc2-deficient (Muc2−/−) mice to understand how A/E bacteria interact with Muc2 and the mucus layer in vivo, and for the first time to assess the role of these interactions in host defense against this important class of bacterial pathogens. Our studies reveal novel yet fundamental insights into how Muc2 is used by the host to control infection by an A/E bacterial pathogen.
Results
C. rodentium penetrates the mucus layer during infection
While C. rodentium is known to infect the colonic mucosal surface by directly attaching to epithelial cells, its location with respect to the colonic mucus layer has not been previously assessed in situ. To study this, we infected C57BL/6 mice with a green-fluorescent protein (GFP)-expressing C. rodentium, and at 6 days post-infection (DPI) we euthanized mice and fixed large intestinal tissues in Carnoy's fixative, which preserves the mucus layer [34]. To maximize our ability to visualize the bacteria, we conducted dual immunostaining for GFP to label C. rodentium, and murine Muc2 to label the inner and outer mucus layer. In uninfected tissues, no GFP-staining was observed, confirming the specificity of the GFP antibody (Figure 1, top panels). However, during infection, we found GFP-C. rodentium widely spread in the outer mucus layer, as well as interspersed throughout the normally sterile inner mucus layer often in proximity to infected epithelial cells (Figure 1, bottom panels). These are the first studies to definitively show C. rodentium within and ultimately crossing both colonic mucus layers in situ. Since C. rodentium is able to circumvent the mucus barrier, we sought to more clearly define whether this Muc2-rich layer actually protects the host, by infecting mice genetically deficient in Muc2.
Muc2-deficient mice exhibit heightened susceptibility to C. rodentium infection
We first infected C57BL/6, Muc2+/+ mice and Muc2−/− mice with C. rodentium and monitored body weights and survival over the first 2 weeks of infection. Since we did not detect any significant phenotypic differences between C57BL/6 and Muc2+/+ mice following infection, we will subsequently refer to these mice as wildtype (WT) mice. As shown in Figure 2A, infected WT mice displayed a slight drop in weight at 2 DPI, followed by recovery and a progressive weight gain over the following week. In contrast, Muc2−/− mice steadily lost weight as their infection progressed. By 6 to 10 DPI Muc2−/− mice had lost on average over 15% of their initial body mass (Figure 2A). This was associated with several clinical signs of morbidity, including hunched posture, bloody diarrhea, and inactivity, to the point where they became moribund and had to be euthanized. Ultimately, depending on the infection, 80–100% of Muc2−/− mice required euthanization, compared to only 0–20% of WT mice (Figure 2B).
We hypothesized that Muc2 secretion and mucus layer formation would limit C. rodentium colonization. Therefore, to address whether the mortality suffered by Muc2−/− mice was associated with increased C. rodentium burdens, we monitored bacterial levels first via bioluminescent imaging of live mice using a luciferase-expressing C. rodentium [35]. Significantly stronger overall signals (3 to 11 fold) were observed emanating from the abdomens of Muc2−/− mice at 4 DPI. (Figure 2C). To verify this by another method, we conducted colony counts on stool samples from mice following oral infection with a streptomycin-resistant strain of C. rodentium. Our results showed significantly increased levels of C. rodentium in the stools of infected Muc2−/− mice, at levels 10 to 100 fold those found in WT mice starting at 2 DPI, and this significance was maintained at 4 and 6 DPI (Figure 2D). Thus, Muc2−/− mice were colonized at a faster rate and to a greater extent than WT mice.
Muc2−/− mice exhibit worsened mucosal damage and microcolony formation on their mucosal surface
Concomitant with the increased bacterial burdens were overt signs of worsened macroscopic damage to the large intestines of infected Muc2−/− mice. This was characterized macroscopically by a shrunken cecum, which in approximately (≈) 60% of mice exhibited focal ulcerations (Figure 3A, arrow, right panel). There was thickening of the descending colon and rectum (colorectal tissue) of infected Muc2−/− mice (Figure 3A left panels), and in ≈40% of mice ulcers were also observed in these regions. Histological analysis of H&E stained sections confirmed the exaggerated damage in the infected Muc2−/− mice: In the cecum there was marked submucosal edema, extensive regions of mucosal hyperplasia, and increased cellular infiltrate throughout the cecal wall (Figure 3B, upper right panel). Similar features were seen in the descending colon and rectum; however, although edema was less overt, there was diffuse damage to the surface mucosa, including ulceration in this region (Figure 3C). The inflammatory cell infiltrate consisted primarily of neutrophils and macrophages as assessed by myeloperoxidase (MPO) and F4/80 staining, respectively (Figure S1A). In contrast, only minimal pathology and reduced inflammatory cell recruitment was observed in infected WT mice (Figure 3A–C; Figure S1A).
The increased damage in infected Muc2−/− mice correlated with enhanced expression of genes encoding inflammatory markers including keratinocyte-derived cytokine (KC), monocyte chemoattractant protein-1 (MCP-1), interferon-gamma (IFN-γ) and tumor necrosis factor-alpha (TNF-α) primarily in the cecum (Figure 3D), and in the colon (Figure S1B). We also assessed the expression of genes encoding colitis-associated cytokines that influence susceptibility to C. rodentium infection, including IL-17A and IL17F [36], IL-22 [37], and IL-23 [38]. The levels of these cytokines were upregulated to a similar degree in infected WT and Muc2−/− compared to uninfected WT mice (Figure S1C). Additionally, the IL-22-regulated lectin regenerating islet-derived III-gamma (RegIII-γ) which can prevent C. rodentium-induced mortality in susceptible mice [37], was also highly upregulated in both strains during infection, and elevated at baseline in uninfected Muc2−/− mice (Figure S1C). Although the large intestinal inflammatory tone (i.e. inflammatory gene expression) of Muc2−/− mice was elevated at baseline relative to uninfected WT mice (Figure 3D, Figure S1B and C), this did not translate to any overt inflammatory cell infiltrate or mucosal damage as determined by histopathological scoring (Figure 3E); however it was accompanied by increased colonic crypt lengths compared to WT mice, as was previously reported [28] (Figure 3C upper left vs. lower left panel), giving rise to the higher score in uninfected Muc2−/− vs. WT mice (Figure 3E). Overall, following infection, histological damage scores were significantly higher in Muc2−/− mice compared to all other groups (Figure 3E).
During our histological examinations, we also noticed focal aggregation of C. rodentium on the mucosal surface of colorectal tissues in Muc2−/− mice, giving rise to bacterial microcolonies, similar to those described by Bry and Brenner [39]. These C. rodentium microcolonies were frequently seen overlying ulcerated mucosal regions (Figure 3C, upper right panel), which were highly populated with neutrophils in direct contact with the microcolonies (Figure S1D). The ulcers also contained macrophages and necrotic epithelial cells (not shown). These microcolonies and ulcers were not observed in infected WT mice (Figure 3C, bottom right panel).
Muc2 deficiency renders mice more susceptible to attenuated C. rodentium strains, although susceptibility is T3SS dependent
We next asked whether the mucosal injury occurred through previously described virulence mechanisms. C. rodentium, as well as other A/E pathogens, is known to cause epithelial injury and apoptosis primarily through the actions of the translocated effector EspF [40], [41]. This effector plays a critical role in causing ulcerations in other susceptible mouse strains [42], so we infected both WT and Muc2−/− mice with wildtype (wt) or ΔespF C. rodentium. As expected, the wt and ΔespF mutant caused minimal morbidity to WT mice as assessed by measurement of weight loss (Figure 4A). In contrast, there was significant weight loss in the Muc2−/− mice infected with ΔespF C. rodentium that was associated with 60% mortality rate, although there was a delay in the onset of these phenotypes compared to wt C. rodentium infection (Figure 4B). Moreover, consistent with these results, there were higher fecal ΔespF C. rodentium burdens in Muc2−/− mice compared to WT mice (Figure 4C). Interestingly, histology revealed that the ΔespF C. rodentium strain also formed the same microcolonies as wt C. rodentium, in concert with focal mucosal ulcerations underlying these overgrowths (Figure 4D). These data indicate that these microcolony-associated ulcerations develop independently of the translocated effector EspF.
To further test the degree of susceptibility of these mice, we infected them with a C. rodentium strain, ΔescN, which is unable to form a functional T3SS and is therefore severely impaired in virulence [43], [44]. In contrast to the ΔespF mutant, ΔescN C. rodentium failed to induce weight loss in Muc2−/− mice, or colonize it to any significant degree (Figure 4E&F). Collectively these results show that Muc2-deficiency renders mice more susceptible to even attenuated A/E bacterial pathogens; however the susceptibility does not extend to strains lacking a functional T3SS.
Muc2 limits initial colonization of the mucosal epithelia, but ultimately controls the levels of luminal bacteria loosely associated with the mucosal tissue
While our histological stains confirmed that C. rodentium crosses the mucus layer to infect the underlying epithelium, the analysis of fecal burdens suggested that Muc2 limits C. rodentium colonization of large bowel epithelium. Consistent with this idea, in vitro studies have shown that rabbit mucins can inhibit EPEC attachment to epithelial cells in culture [45]. These data collectively suggest mucus may play a role in innate host defense by acting as a physical barrier to limit pathogen access to the epithelium. We tested this using an in vivo colonization assay. This was performed through cecal loop surgery in WT and Muc2−/− mice, where the ascending colon was tied off with sutures and 1×108 C. rodentium were injected into the cecum (see also Materials and Methods). Ten hrs later, when the mice were euthanized and the ceca were removed, thoroughly washed of their contents, homogenized and plated, we found significantly greater numbers of adherent bacteria attached to the ceca of Muc2−/− mice compared to WT mice (Figure 5A). These counts were supported by immunostaining for the C. rodentium-derived infection marker translocated intimin receptor (Tir) [46], where a greater mucosal surface area was positive for Tir in the Muc2−/− ceca, compared to WT ceca that exhibited only patchy Tir staining (Figure 5B). These results demonstrate that Muc2 production limits the rate of intestinal epithelial colonization by this A/E pathogen in vivo.
Despite these findings, it was unclear if a doubling in the colonization rate, as seen in the cecal loop model could explain the 10–100 fold increase in total pathogen burdens found in the orally infected Muc2−/− mice. We therefore quantified intimately adherent (i.e. directly infecting epithelial cells) versus luminal (non-infecting) C. rodentium in the cecal and colorectal tissues of orally infected WT and Muc2−/− mice, focusing on 4 and 7 DPI, prior to when Muc2−/− mice become moribund. Unexpectedly, we found no significant difference at either time point in the number of intimately adherent C. rodentium in the large bowel of Muc2−/− mice compared to WT mice (Figure 5C). However there was a significant and dramatic 10-fold increase in the numbers of luminal C. rodentium recovered from Muc2−/− mice compared to WT mice (Figure 5C).
To clarify what these burdens meant with respect to how C. rodentium interacted with the mucosa in situ, we stained for C. rodentium lipopolysaccharide (LPS) as well as the infection marker Tir. Immunostaining at 4 DPI showed that in both strains, C. rodentium primarily infected the mucosal surface (Tir-positive), but did not invade the crypts (Figure 5D). Interestingly, while there was significantly more LPS staining in Muc2−/− tissues, most of the staining was focused in patches where large numbers of C. rodentium accumulated on the mucosal surface, although only a small fraction of these bacteria expressed Tir and were thus directly attached to and infecting the epithelium (Figure 5D, bottom panels). These results indicate that Muc2 deficiency does not significantly impact the total number of bacteria that ultimately infect the tissue, but predisposes the large bowel to greater numbers of loosely (i.e. non-epithelial) adherent bacteria on the mucosal surface, giving rise to the increased overall luminal burdens. As the infection progressed to 6 DPI, when mice started to become moribund, it appeared that the microcolonies were more invasive, as they penetrated deeper into the crypts and were more frequently associated with ulcerated regions (not shown, and Figure 3). Thus the propensity to accumulate bacteria on the surface of a Muc2-deficient mucosa is likely a key contributory factor to the ulcer development that occurs in these mice during infection.
The increased luminal C. rodentium burdens in Muc2−/− mice are not due to intrinsic defects in antimicrobial activity at their mucosal surface
We have shown that the mucus layer provides a structural barrier that limits initial C. rodentium attachment in vivo; however, this barrier effect does not readily explain the accumulation of loosely adherent bacteria and microcolony formation at the mucosal surface of Muc2−/− mice. One plausible explanation for these overgrowths is an overall reduction in antimicrobial activity at the epithelial surface. To assay antimicrobial production in Muc2−/− mice, we first looked at the gene expression levels for epithelial-derived murine cathelicidin-related antimicrobial peptide (mCRAMP) and inducible nitric oxide synthase (iNOS), which have been shown to play a role in controlling C. rodentium levels in vivo [13], [47]. We did not see any significant differences in the expression of cnlp (mCRAMP), between mouse strains however, and the expression of inos was higher in Muc2−/− mice (Figure 6A). These data were supported at the protein level by immunostaining (not shown), indicating that the loss of Muc2 does not result in overt defects in the expression or production of innate factors known to control this pathogen.
An alternative explanation could be that Muc2 is essential for controlling pathogen numbers on the colonic surface by mediating direct antibacterial activity as shown for gastric mucus against Helicobacter pylori [44], and/or indirect activity by acting as a matrix to strategically position host defense peptides, as recently shown for small bowel mucus [43]. To address this in the large bowel, we tested the antimicrobial activity of crude mucus isolated from the colorectal tissues of WT uninfected mice, in a manner similar to that described by Meyer-Hoffart et al. [48]. Interestingly, we found no evidence that the crude colonic mucus had any antimicrobial activity against C. rodentium; instead, the addition of the mucus actually led to increased C. rodentium growth, likely by acting as a nutrient source (Figure 6B).
Mucus secretion is increased in response to C. rodentium infection
In the absence of antimicrobial activity by the mucus layer, another mechanism by which Muc2 could limit luminal numbers of C. rodentium is by binding to and mechanically flushing C. rodentium out of the colon. It has already been shown that intestinal mucus binds with high affinity to pathogens [49] including C. rodentium [19], and that bacterial products [50] as well as host factors stimulate mucin release both in vitro and in vivo [51]. Therefore, we hypothesized that enhanced mucus secretion could be key to the rapid removal of loosely adherent C. rodentium from the mucosal surface. To determine if we could see evidence of this histologically, we first conducted periodic acid-Schiff (PAS) staining on Carnoy's-fixed colorectal sections from uninfected and C. rodentium-infected mice at 6 DPI. As shown in Figure 7A, infected WT mice showed evidence of increased luminal mucus staining compared to uninfected mice.
To quantify this increased mucus production, we conducted pulse-chase experiments using [3H]-glucosamine injections in mice to metabolically label glycoproteins such as mucins in uninfected and infected mice. Mucin secretion was analyzed at 6 DPI when bacteria exhibit uniform colonization of the distal colorectal mucosa. At 3.5 hrs post-injection of [3H]-glucosamine, we extracted total secretions from the entire colon of control and infected mice, and quantified the secretions via scintillation counting. We observed ≈30% higher total counts per minute (CPM) in secretions from infected vs. uninfected mice (Figure 7B). To determine how this related to mucin vs. non-mucin production, we subjected the [3H]-labeled secretions to fractionation on a Sepharose 4B column calibrated with blue dextran (fractions 17–22), and ovalbumin (fractions 30–35) where mucins are eluted in the void volumes (Vo) and non-mucin glycoproteins are eluted in later fractions (Vt) [52]. Graphical analysis of the fractions (Fraction # vs. CPM), revealed a higher amplitude and larger breadth of the peak of the Vo fractions (#13–21) of D6-infected mice compared to uninfected controls (Figure 7C). This translated to an average 40±10% increase in [3H]-labeled mucin in the pooled high molecular weight Vo fractions in infected mice (Figure 7D).
To visualize how mucus secretion specifically impacts host-pathogen interactions, we conducted dual epifluorescent staining for C. rodentium LPS and Ulex europaeus agglutinin UEA-1, which binds to fucosylated residues abundant in mucus. Staining was performed on colorectal tissues at 6 DPI in WT mice in heavily infected regions where Muc2/mucus responses were underway. Supporting and extending the findings of previous reports [19], [44] we identified a single layer of C. rodentium infecting the epithelium, with no signs of microcolony formation. Instead numerous individual C. rodentium were seen intermixed within the luminal mucus directly above but not in contact with intimately adherent bacteria (Figure 7E, left panel and inset). In stark contrast, when we conducted UEA-1/LPS staining in Muc2−/− mice (6 DPI) we found that, although there were UEA-1 positive hypotrophic goblet cells, the crypt lumens were devoid of mucus as expected, and the absent mucus was replaced by a C. rodentium microcolony on the surface epithelium (Figure 7E, right panel). These results strongly suggest that secretion of mucus is important for removing loosely associated bacteria from the mucosal surface.
Although Muc2 is the major secreted mucin in human and mouse colon under baseline and inflammatory conditions [27], [53], [54], other intestinally expressed mucins may also contribute to the secreted mucin pool. We assessed the gene expression of several mucins that have been implicated in C. rodentium infection, and/or that are up-regulated in colitis, including the cell-surface mucins Muc1 and Muc3/17, and Muc13 [19], and the secreted non-gel forming mucin Muc4 that can be expressed by goblet cells [19], [55]; we also looked mucins that have gel-forming capacity, including the secreted gel-forming salivary and gastric mucins Muc19 [56] and Muc6 [57] respectively. There were no major changes in any of these mucins except for Muc6, which was elevated in Muc2−/− mice at baseline and also increased in WT mice during infection relative to uninfected WT controls (Figure S2A). However, because PAS staining revealed a virtual absence of mucin-filled phenotypically distinct goblet cells, and luminal mucus, under uninfected and infected conditions in Muc2−/− mice compared to WT mice, Figure S2B), this suggests that the expression of other mucins, particularly secreted gel forming mucins, do not compensate for the loss of Muc2 during C. rodentium infection.
Muc2 secretion regulates commensal and pathogen numbers in the large bowel lumen
Uninfected Muc2−/− mice have been shown to exhibit commensal bacteria interacting with their mucosal surfaces more frequently than WT mice [27]. Interestingly, following staining for C. rodentium LPS within the microcolonies, we noted numerous LPS-negative bacteria intermixed with the positively staining bacteria (Figure 8A), suggesting these microcolonies contained other bacterial species in addition to C. rodentium. To test this we conducted dual fluorescence in situ hybridization (FISH) staining on colorectal sections of infected Muc2−/− mice as well as WT mice after infection using a Texas-Red conjugated EUB338 probe that recognizes 99% of all bacteria, as well as an AlexaFluor 488-conjugated GAM42a probe that detects γ-Proteobacter, the class to which C. rodentium belongs [58]. Our results show that in regions of microcolony formation in infected Muc2−/− mice, the majority of bacteria were EUB338+GAM42a+ (C. rodentium, yellow), but there were distinct clusters of EUB338+GAM42a– (commensal, red) bacteria mixed in with the EUB338+GAM42a+ cells, confirming that these microcolonies contain non-C. rodentium bacterial species (Figure 8B, left panels). Moreover, numerous commensal species could be seen interacting with the epithelium in other regions (not shown). In contrast, in WT mice (6 DPI) the epithelial surface was primarily colonized with EUB338+GAM42a+ cells as expected (Figure 8B, right panel); and while scattered EUB338+GAM42a– bacteria were occasionally seen in the luminal mucus or near the surface, we did not observe them forming microcolonies with C. rodentium or interacting with the mucosal surface as we observed in Muc2−/− mice.
The above results suggest that if Muc2 promotes host defense by flushing C. rodentium away from the mucosal surface and out of the colon, then most enteric microbes, including commensals, would be affected by such a response. Recent studies have shown that C. rodentium induced colitis causes dramatic, host-mediated changes in the commensal bacterial communities in the murine colon, including a significant reduction in total commensal numbers [58]. To test whether Muc2 plays a role in this response we measured bacterial numbers within the colorectal lumen via SYBR green staining in uninfected and infected WT and Muc2−/− mice. Our results show comparable bacterial densities in the colons of uninfected WT and Muc2−/− mice (Figure 8C). During infection of WT mice, the density of total luminal bacterial numbers began decreasing over the course of infection, with a ≈40% reduction evident by 6 DPI, consistent with the findings of Lupp et al. [58]. In contrast, there was a ≈30% increase in the total luminal bacteria recovered from Muc2−/− mice, a density significantly greater than that recovered from WT mice (Figure 8C). Analysis of the colorectal luminal contents revealed that although the percent composition of γ-Proteobacter, most of which are C. rodentium [58], [59], in the Muc2−/− mice was slightly greater compared to WT mice (Figure 8D) the vast majority (97%) of the bacteria in both mouse strains were commensals. Thus, Muc2−/− mice do not undergo the commensal loss seen in the WT mice, and in fact, exhibit a trend toward increased numbers compared to uninfected controls, although this was not significant. As the infection progressed up to 10 DPI in the Muc2−/− mice, FISH staining revealed that the mucosa became covered with a thick biofilm of pathogenic microbes mixed in with commensal bacteria (Figure 8E), which was never observed in WT mice. These results collectively suggest that during infection, Muc2 plays a critical role in regulating both pathogen and commensal interactions at the mucosal surface.
Exaggerated barrier disruption and translocation of pathogenic and commensal bacteria in infected Muc2−/− mice
Next, we examined the factors potentially responsible for the high mortality rates seen in infected Muc2−/− mice. We speculated that the increased numbers of luminal and surface-associated bacteria would not on their own cause the deaths of Muc2−/− mice, however the association of the loosely-associated overgrowths with superficial ulceration (Figure 3C) suggested that infection-induced epithelial barrier disruption and bacterial translocation might play a causal role in their mortality. To assess this potential, we infected WT and Muc2−/− mice and at 5 DPI we orally gavaged the mice with fluorescein isothiocyanate (FITC)-Dextran (4 kDa) (FD4) and assessed the translocation of FD4 from the gut lumen into the serum. Our results showed a striking and significant increase in the amount of FD4 in the serum of infected Muc2−/− mice compared to infected WT mice and uninfected Muc2−/− mice (Figure 9A). These results demonstrate that C. rodentium infection leads to a dramatic increase in intestinal permeability in the absence of Muc2. As expected, we saw similar results in response to ΔespF C. rodentium (not shown). To determine whether the exaggerated barrier disruption seen in Muc2−/− mice led to greater systemic pathogen burdens, we analyzed systemic sites, including the spleen, liver and mesenteric lymph nodes (MLNs) at 6 DPI. We found significantly higher C. rodentium burdens in the spleen, liver, and a trend toward higher burdens in the MLNs in infected Muc2−/− vs. WT mice (Figure 9B). We also found consistently higher colony forming units (CFUs) of C. rodentium isolated from whole blood of Muc2−/− mice that was plated directly after cardiac puncture (Figure 9C).
Since increased commensal numbers were observed loosely associated with the epithelial surface, we examined their interactions with the damaged tissue by FISH as above. When we stained the ulcerated regions, we observed EUB338+GAM42a– (commensal) bacteria interacting with numerous invasive EUB338+GAM42a+ (C. rodentium) microcolonies, and both were found amidst a dense population of polymorphonuclear leukocytes (PMNs) (Figure 9D). Numerous bacteria were also seen within the cell bodies of PMNs (Figure 9D, insets “a” and “b”). At times of barrier disruption, large numbers of both C. rodentium and non-γ-Proteobacter species could be found deep within the mucosa of infected Muc2−/− mice (Figure 9E). Rarely if ever were microbes observed in the mucosa of infected WT mice. These results strongly suggest that both pathogenic and commensal bacteria contribute to the disease and mortality suffered by Muc2−/− mice, since A/E bacterial infection-induced disruption of the epithelial barrier allows massive translocation of both pathogenic and commensal bacteria out of the intestinal lumen and into mucosal tissues, and pathogens into systemic compartments, leading to bacteremia.
Evidence that Muc2-deficiency reduces host-mediated pathogen clearance when commensal-dependent host colonization resistance is compromised
The above data show commensal and pathogenic bacteria occupying intestinal niches in Muc2−/− mice that are not colonized in WT mice during infection. To attempt to elucidate the precise role of commensal bacteria during C. rodentium infection in Muc2−/− mice, we administered a high dose of the antibiotic streptomycin (20 mg/mouse) by oral gavage to reduce the numbers of total commensals prior to infection. Stool was collected immediately prior to treatment and again 24 hrs later, and then stool bacteria was quantified as above to confirm commensal depletion. Streptomycin (strep) treatment resulted in a significant (average 10–20 fold) reduction in commensal bacterial numbers in both WT and Muc2−/− mice, while vehicle treatment did not cause any significant changes (Figure 10A). Neither treatment led to any inflammation or pathology on its own when assessed 7 days later (not shown). 24 hrs after treatment, strep- and vehicle-treated WT and Muc2−/− mice were also gavaged with ΔespF C. rodentiumStr (strep-resistant), which was chosen instead of wt C. rodentium because it is less virulent. Colonization was assessed by plating stool contents every second day. The results show that at 2 DPI, strep-treated WT and Muc2−/− mice carried 10–50 fold higher bacterial burdens compared to infected vehicle-treated WT and Muc2−/− mice (Figure 10B). However by 4 and 6 DPI, while ΔespF C. rodentiumStr burdens began to decline in infected strep-treated WT mice ultimately to levels similar to infected vehicle-treated WT mice (6 DPI), bacterial burdens in infected strep-treated Muc2−/− mice continued to increase to levels significantly higher than all other groups (Figure 10B). Moreover, burdens in infected vehicle-treated Muc2−/− mice also increased to levels that were higher than infected strep-treated WT mice at 6 DPI. Although weight loss varied among mice both Muc2−/− groups, only WT mice tended to gain weight during infection (Figure 10C).
At 6 DPI, both cecal and colonic tissues were resected and assessed by histology. As shown by H&E (Figure 10D, bottom panels), strep-treatment led to increased edema and inflammation in WT ceca compared to vehicle-treated WT mice during infection; however in infected Muc2−/− tissues, there were no obvious differences in cecal and colonic inflammation between strep-and vehicle-treated groups (Figure 10D, top panels). Overt ulceration was seen in the ceca of vehicle-treated Muc2−/− mice (Figure 10E), while ulcers were observed in the colons of strep-treated Muc2−/− mice (Figure 10F) Interestingly, FISH staining of cecal sections from infected vehicle-treated Muc2−/− mice showed large numbers of commensals (EUB338+GAM42a–, red) directly interacting with PMNs in ulcerated regions (Figure 10E, left panel). These interactions were seen at the mucosal surface of ulcers where there was little evidence of ΔespF C. rodentium; however ΔespF C. rodentiumStr could still be seen within the PMNs (Figure 10E, right panel, inset). In contrast, large invasive ΔespF C. rodentiumStr microcolonies (EUB338+GAM42a+, yellow) could be seen associated with the ulcers in the colons of infected strep-treated Muc2−/− mice (Figure 10F, right panel). Such pathology was never observed in uninfected mice or in any of the infected WT groups. Collectively, these results indicate that (i) Muc2 promotes host-mediated colonization resistance when commensals are depleted; and (ii) commensal bacteria, although initially important in promoting colonization resistance in both strains, ultimately come into direct contact with large numbers of PMNs following the infection-induced ulceration that occurs in a Muc2-deficient environment. Thus Muc2 is critical for managing commensal and pathogenic bacteria within the GI tract, particularly at mucosal surfaces during an enteric infection.
Discussion
The Muc2-rich mucus layer is the first host-defense barrier that noxious luminal agents contact in the intestine [33], and as such, it functions as the main interface between the host and its luminal microbiota. To our knowledge, this is the first study to formally demonstrate the importance of the major mucus glycoprotein Muc2 in host defense against an A/E bacterial pathogen in vivo. We show that the presence of Muc2 and hence the mucus layer is necessary to protect against severe mucosal damage and barrier dysfunction during infection. This was in part due to Muc2 functioning as a structural barrier to limit the rate of pathogen colonization of epithelial cells in the large bowel. However, Muc2 plays an additional role in host defense by controlling the pathogen burden that resides within the colonic lumen, primarily by removing loosely adherent bacteria and preventing bacterial accumulation and microcolony formation on the colorectal surface. The inability to effect this removal likely contributes to the severe barrier dysfunction seen in Muc2−/− mice. We provide evidence that the ability of Muc2 to control luminal bacteria is most likely attributable to increased Muc2/mucus secretion during infection, which was demonstrated through metabolic labeling of mucin glycoproteins in WT mice. Moreover, we demonstrate that the ability of Muc2 to control luminal pathogens also impacts the resident commensal microbiota, as the microcolonies seen overlying the mucosa of infected Muc2−/− mice contained both C. rodentium as well as commensal microbes, and both types of bacteria were seen translocating across the colonic epithelium and into the lamina propria. These results ultimately reveal Muc2 production as a critical mechanism by which the host controls exposure to both pathogenic and commensal bacteria in vivo.
While we assumed that A/E pathogens such as C. rodentium would have to interact with the mucus layer during the course of infection, we demonstrate and characterize this interaction for the first time in situ. We show that C. rodentium colonizes the outer mucus layer in high numbers, and can also be found traversing the normally bacteria-free inner mucus layer to gain access to the underlying epithelial cells. These results raise the question of how A/E pathogens manage to circumvent the mucus layer. C. rodentium lacks a functional flagellum and is thus non-motile [60], and therefore likely utilizes specific mucinases or glycosidases to digest mucin in order to overcome the mucus barrier, although this has yet to be formerly demonstrated. Notably, EHEC has recently been shown to secrete the metalloprotease StcE that has apparent mucinase activity [61] suggesting A/E pathogens do employ this strategy. In contrast, despite their diversity and extreme density in mammalian colon, commensal bacteria do not penetrate the inner mucus layer to any significant degree, probably because they are more adapted to the nutrient-rich luminal environment [62]. Ultimately, this suggests that colonizing the outer and inner mucus layer is a key step for the pathogenesis of A/E bacteria, therefore, the bacterial factors involved in crossing the mucus layer are likely critical for virulence.
Our studies reveal an unexpected insight into how Muc2 mediates protection. Muc2 is widely presumed to act as a physicochemical barrier to limit access to epithelial tissues by luminal pathogens [17], including pathogens such as A/E bacteria. Several lines of evidence support this, such as the demonstration of mucins inhibiting EPEC adherence in vitro [20] and our in vivo cecal loop colonization assay described in this report. However, since the total numbers of bacteria that ultimately infected (i.e. became intimately adherent to) the tissue was not significantly different in a Muc2 deficient environment, the role of Muc2 as a defense barrier may be of only transient importance. Rather the major function played by Muc2, at least in response to A/E bacteria, appears to be to limit luminal burdens, mainly by preventing the accumulation of pathogens that are loosely associated with the tissue. These bacteria probably arise from replication of intimately-bound pathogens, as the T3SS mutant (ΔescN C. rodentium) failed to efficiently colonize. This massive increase in the overall pathogen burden at the mucosal surface has important implications for downstream host responses. EPEC and EHEC both disrupt epithelial permeability in vitro [63], as does C. rodentium in vivo [64], [65]. While intimately-adherent bacteria are firmly bound to the epithelia, the non-infecting, but loosely adherent bacteria are more likely to translocate into the mucosa, particularly when faced with the mechanical pressures of dietary flow. Indeed, at times of severe barrier disruption we saw much higher systemic levels of C. rodentium in the Muc2−/− mice.
Although Muc2 deficiency did not ultimately impact on the numbers of intimately-adherent C. rodentium, there was a striking increase in intestinal permeability in Muc2−/− compared to WT mice. The susceptibility to ulcer formation in the Muc2−/− mice is probably a major contributor to the barrier dysfunction and morbidity seen in these mice since it was associated with greater systemic pathogen burdens. While the mechanisms are unclear, we suggest the accumulation of bacteria and microcolony formation on the epithelial surface in a Muc2-deficient environment is linked to either the development and/or maintenance of the ulceration, since most ulcers were associated with the microcolonies. It has been proposed that serum proteins released at ulcerated sites contribute to ulcer-associated C. rodentium overgrowth [66]; however the fact we saw microcolony formation also in non-ulcerated sites argues against this always being the case. Interestingly, past studies have shown that the A/E pathogen translocated effector EspF has been linked to epithelial barrier disruption [41], [67] and ulcer-associated damage [42]. However, since ulcers, microcolony formation, and barrier disruption were also seen in mice infected with the ΔespF strain, these data indicate that barrier disruption occurs through non-canonical pathways. We speculate that bacterial accumulation and microcolony formation at the surface adversely affects epithelial survival either directly, by producing a high local concentration of toxic metabolites; or indirectly, by causing the recruitment of large numbers of PMNs to the site of infection, where epithelial cell death is the result of collateral damage caused by neutrophils releasing cytotoxic mediators to control the infection. In fact, one can envision these microcolonies to be an overwhelming burden to recruited phagocytes, perpetuating a vicious inflammatory cycle (Figure 10). Whatever the specific role of these invasive microcolonies, they likely exacerbate the focal damage and associated barrier defects, and thus have a severe impact on morbidity in the Muc2−/− mice.
Although we attribute the majority of the pathological phenotypes in infected Muc2−/− mice to result from C. rodentium, one of the striking features during the course of infection was the maintenance of commensal bacteria at the mucosal surface of the Muc2−/− mice. While we also found scattered commensal bacteria overlying the epithelium before infection [27], C. rodentium was clearly unable to totally displace them. This led to some intriguing phenotypes, including direct intimate interactions between commensal bacteria and the pathogen, where commensals were found intermixed with C. rodentium clusters to create multispecies microcolonies. Critically, commensal species could also be found translocating across the mucosal surface and into the lamina propria, where they were in direct contact with PMNs at sites of microcolony-associated ulceration, even forming microcolonies of their own. We explored whether these commensal bacteria contribute to the resulting colitis by transiently depleting them using the antibiotic streptomycin. While the depletion was successful, it also led to an exaggerated pathogen burden, confirming that commensal bacteria play an important host defense role by providing colonization resistance against C. rodentium. Although we did not identify overt differences in the resulting pathology in Muc2−/− mice following antibiotic pretreatment, we were unable to conclude to what degree commensal translocation might play in the resulting colitis, considering the loss of commensals occurred concomitantly with increased pathogen burdens. However, the fact that infection-induced cecal ulceration in Muc2−/− mice led to large numbers of commensals that were directly interacting with PMNs points to a pathologic host-commensal interaction during infection. Therefore, while commensals are beneficial early during an infection by enhancing colonization resistance, their continued presence as the infection progresses likely plays a pathologic role. These studies are particularly interesting in light of the study by Lupp et al. [58] who described an overall reduction of commensal bacterial numbers after an established C. rodentium infection. It has been suggested this is a pathogenic strategy where pathogens exploit inflammation to suppress commensal growth and thereby reduce colonization resistance [68]. However, our findings strongly suggest that clearance of commensal microbes from the colon after an established C. rodentium infection may also benefit the host, by decreasing the total bacterial burden faced by the host at a time when its intestinal barriers are compromised.
We hypothesize that mucin secretion is the key mechanism by which Muc2 controls the levels of A/E bacteria, and commensal bacteria at the surface. Recent studies have suggested there is enhanced mucin secretion in the colon during C. rodentium infection [19]. We extend these findings through metabolic labeling to show at least a 40% increase in mucus secretion in response to infection, and specifically indentified C. rodentium within luminal mucus. This increase in mucin secretion is likely a gross underestimate of the local increase in mucin release, since in order to have sufficient quantities for analysis, we extracted mucus from the whole colon, and the increase in secretion is expected to be focused in the descending colon and rectum where the infection occurs [69]. Due to the lack of antimicrobial activity we saw within the crude mucus, and the fact that it was recently shown by the McGuckin laboratory that C. rodentium directly binds to Muc2/mucus in vitro [19], we hypothesize that induced mucin secretion is an effective means for the host to bind and remove non-infecting, loosely adherent A/E bacteria that would otherwise accumulate on the surface and exacerbate disease (Figure 11). Although beyond the scope of the present study, an outstanding issue yet to be addressed is deciphering the precise molecules responsible for the induced Muc2 secretion in vivo. There are a plethora of candidates, including bacterial products, such as LPS [51], [70], or host derived cytokines such as TNFα [71], neuromodulators including vasoactive intestinal peptide [72], or neutrophils via elaboration of secretagogues such as neutrophil elastase [73], all of which have been shown to cause enhanced mucin release from goblet cells in tissue culture, and are present during C. rodentium infection [74], [75], [76]. Based on the data presented in our report, the elucidation of the specific host and/or microbial factors and molecular pathways that regulate mucus production during enteric bacterial infection constitutes a fertile area of research.
Importantly, while we ascribe the ability of intestinal mucus to flush away luminal bacteria from the mucosal surface to primarily reflect the actions of Muc2, there are likely other mucins, (potentially found in total secreted mucus) that may also contribute to the protective actions of the mucus. These include Muc1, a cell surface mucin that is upregulated in bacterial induced colitis [19] and potentially cleaved to release its α-subunit containing the extracellular mucin domain into the intestinal lumen, as seen during H. pylori infection [77]; Muc4 which can be up-regulated during DSS-induced colitis [78]and be expressed by colonic goblet cells [55]; and the secreted gel-forming mucins Muc19 and Muc6, the latter being produced in Muc2−/− mice during colitis [29]. Even so, we maintain that Muc2 is the major protective mucin. This is in part based upon the phenotype of Muc2−/− mice (confirmed by our studies), where Muc2-deficiency leads to a virtual loss of mucin-filled phenotypically mature goblet cells within the large intestine, and a corresponding loss of both the inner and outer colonic mucus layers [24] and other forms of luminal mucus. Moreover, Muc2 is by far the major secretory mucin under both baseline (in mice and humans) [24], [53] and inflammatory conditions in the colon [54]. However, we did see a modest up-regulation of Muc6 mRNA expression during infection of WT mice, and the impact of this expression is currently under investigation.
During the course of this article review, it was demonstrated by Hasnain et al. [79] that Muc2-deficiency renders mice more susceptible to intestinal nematode infections, suggesting Muc2 and mucus production can protect against diverse enteric pathogens. Muc2 production is clearly protective during A/E bacterial infection, but whether this is true for other enteric bacterial pathogens of the gut remains to be shown. Importantly, since bacteria and other enteric pathogens have co-evolved with their hosts, many exhibit multiple strategies to subvert and exploit host defenses including mucus to promote colonization [80]. A well known motility factor is flagella, which is commonly utilized by pathogenic bacteria such Vibrio cholerae to migrate through mucus (reviewed in [33]). In addition, Salmonella appears to anchor itself to mucus via specific adhesins [81] to promote colonization [82], and exhibits resistance to small bowel mucus antimicrobial activity [48]. Yersinia enterocolitica has been shown to utilize polysaccharides present in mucins like Muc2 to harvest energy and promote growth [83]. A similar observation has been shown for Salmonella Typhimurium, for which it has been proposed as a strategy to outcompete the commensal microbiota within an inflammatory niche [84]. Moreover, parasites such as Entamoeba histolytica stimulate mucin release to deplete the mucus layer [85], as well as proteolytically break down the polymeric structure of secreted Muc2 to facilitate access to the underlying epithelium [86], [87]. Thus, whether Muc2 has evolved primarily to regulate interactions with normal microbiota and other luminal contents, or to provide adequate host defense against enteric pathogens has yet to be determined. However, because the commensal microbiota is a major variable in any enteric infection, particularly in the colon, it is likely that the presence of Muc2 allows for effective immunological management of the infectious agent by limiting commensal burdens at mucosal surfaces.
In conclusion, our studies have shown that Muc2 and the mucus layer are critical for host defense against an A/E bacterial pathogen. However, it is important to note that Muc2 can potentially be modulated in several ways either during infection, such as at the level of gene expression, post-translational modification, or even at the level of secretion into the intestinal lumen. Each regulatory step may influence the biological function of Muc2, which in turn will influence how the host responds to enteric pathogens. Since Muc2 is an integral part of the colonic ecosystem, future studies are warranted to unravel precisely how intestinal mucus impacts the course of infectious disease.
Materials and Methods
Mice
Six to eleven-week-old C57BL/6, Muc2+/+ and Muc2−/− mice (on C57BL/6 background) were purchased from the National Cancer Institute (NCI) or bred in our animal facility. Mice were kept in sterilized, filter-topped cages, handled in tissue culture hoods and fed autoclaved food and water under specific pathogen free (SPF) conditions. Sentinel animals were routinely tested for common pathogens. The protocols employed were approved by the University of British Columbia's Animal Care Committee and in direct accordance with guidelines drafted by the Canadian Council on the Use of Laboratory Animals.
Bacterial strains and infection of mice
Mice were infected by oral gavage with 0.1 ml of an overnight culture of LB containing approximately 2.5×108 cfu of wt C. rodentium (formerly C. freundii biotype 4280, strain DBS100, the EspF mutant ΔespF C.rodentium, or the T3SS mutant ΔescN C. rodentium [88]. Bioluminescent strains of C. rodentium were constructed by introducing plasmid pT7 (E. A. Meighen, Department of Biochemistry, McGill University) carrying the entire lux operon from Photorhabdus luminescens. For bacterial enumeration studies, a streptomycin-resistant derivative of C. rodentium DBS100 was utilized. For some studies a streptomycin-resistant ΔespF C.rodentium was utilized, and was constructed in our laboratory by routine procedures. GFP-C. rodentium was constructed within our laboratory by chromosomal insertion of gfp into C. rodentium DBS-100 via Red/ET Recombination, using a Quick & Easy E. coli Gene Deletion Kit (Gene Bridges) as per manufacturers instructions. The virulence of the GFP-C. rodentium was confirmed in preliminary studies. For commensal depletion studies, mice were pre-treated with 0.1 ml of 200 mg/ml (20 mg) streptomycin (or H20) 24 hrs prior to infection.
Tissue collection
Uninfected or infected mice were anesthetized with Halothane, killed by cervical dislocation, and their large intestines were resected and divided into cecum, ascending colon, descending colon, and rectum for further analysis. Tissues were immediately placed in 10% neutral buffered formalin (Fisher) (48 hrs, 4°C) or ice cold fresh Carnoy's Fixative (2 hrs, 4°C) or 4% paraformaldehyde (1 hr, room temp) for histological studies, or placed in RNAlater (Qiagen) and stored at −86°C for subsequent RNA extraction.
Bacterial counts
For enumeration of bacteria within the tissue and luminal compartments, whole mouse ceca or colons were sliced open longitudinally, and their luminal contents were collected in a preweighed 2.0 ml microtube containing 1.0 ml of phosphobuffered saline (PBS) and a 5.0 mm steel bead (Qiagen). Cecal and colonic tissues were washed vigorously in PBS (pH 7.4), cut into several pieces, and also placed in a tube as above. Tissue and lumen contents were weighed, and then homogenized in a MixerMill 301 bead miller (Retche) for a total of 6 mins at 30 Hz at room temperature. Tissue homogenates were serially diluted in PBS and plated onto luria broth (LB) agar plates containing 100 mg/ml streptomycin, incubated overnight at 37°C, and bacterial colonies were enumerated the following day, normalizing them to the tissue or stool weight (per gram). For fecal bacterial burden analysis, stool was collected from live mice at various times post-infection (described in text) and processed as described for luminal contents. For some studies with non-antibiotic resistant C. rodentium, plating was performed on MacConkey Agar (Difco), C. rodentium colonies were clearly identified by their unique characteristic of being round with red centre and a thin white rim. Colonies were confirmed to be C. rodentium by PCR for the C. rodentium T3SS translocator gene escN.
Histological staining
Briefly, 5 µm paraffin sections were deparaffinized by heating at 55–65°C for 10 min, cleared with xylene, rehydrated through an ethanol gradient to water. Sections were blocked using the appropriate blocking buffer (either 2% Goat or Donkey Serum in PBS containing 1% bovine serum albumin (BSA), 0.1% Triton-X100 (Sigma), and 0.05% Tween 20, and 0.05% sodium azide. For detection of biotinylated targets, blocking of endogenous biotin was carried out prior to blocking with serum, using the Endogenous Biotin Blocking kit (Molecular Probes). Primary antibodies or lectins were diluted in PBS containing 1% BSA, 0.1% Triton-X100 (Sigma), and 0.05% Tween 20, and 0.05% sodium azide. The antibodies used were rat anti-F4/80 (1∶8000; Serotec), rabbit anti-MPO (1∶100; NeoMarkers), rat antisera generated against C. rodentium specific Tir (1∶5K; gift from W. Deng), rabbit anti-E.coli Poly 8 LPS (1∶500; Biotec Laboratories), biotinylated goat anti-GFP (1∶100: GeneTex), polyclonal antisera that recognized the murine colonic mucin Muc2 (1∶50; a gift from Jan Dekker). Staining for fucosylated mucins was carried out using biotinylated-Ulex europaeus agglutinin-1 (2 ug/ml; Vector Labs). Antigen retrieval was used for F4/80 and MPO staining, and was performed prior to blocking and staining by placing deparaffinized, rehydrated slides in 10 mM citric acid pH 6.0 at 90–100°C for 20 min, followed by cooling to room temperature. Preparation and staining of PFA-fixed frozen sections was performed as described previously [44]. For dual LPS/Tir staining, no detergents (TritonX-100 or Tween-20) were used in the dilution buffers, to avoid Tir staining within bacteria. Epifluorescent labeling for all stains was carried out with the appropriate secondary antibody using AlexaFluor 488-conjugated goat (or donkey) anti-rabbit IgG, AlexaFluor 568-conjugated goat anti-rabbit IgG, AlexaFluor 568-conjugated goat anti-rat IgG (all 1∶2000), or AlexaFluor 568-conjugated Streptavidin (1∶1000) (Molecular Probes/Invitrogen). Tissues were mounted using ProLong Gold Antifade reagent (Molecular Probes/Invitrogen) that contains 4′,6′-diamidino-2-phenylindole (DAPI) for DNA staining. Sections were viewed at 350, 488, and 594 nm on a Zeiss AxioImager microscope. Images were obtained using a Zeiss AxioImager microscope equipped with an AxioCam HRm camera operating through AxioVision software (Version 4.4).
RNA extraction and quantitative RT-PCR
Colon tissues stored in RNAlater (Qiagen) at −86°C were thawed, weighed, and total RNA extracted using the Qiagen RNeasy kit following the manufacturer's instructions. Tissues were homogenized in a 2.0 ml microtube containing 0.6 ml of Buffer RLT (supplied in Qiagen RNeasy kit) and a 5.0 mm steel bead (Qiagen), and homogenized in a MixerMill 301 bead miller (Retche) for 4 minutes at 30 Hz at room temperature. Total RNA was quantified using a NanoDrop Spectrophotometer (ND1000). 1–2 ug of RNA was reverse-transcribed using a Qiagen Omniscript RT kit (Qiagen), according to manufacturer's instructions. For quantitative PCR, cDNA was diluted 1∶5 in RNase/DNase free H2O and 5 µl was added to 15 µl PCR reaction mix. The final reaction volume was 20 uL, containing BioRad Supermix used at a 1∶2 dilution, and primers at a final concentration of 0.6 uM each. qPCR was carried out using a BioRad Miniopticon or Opticon2. Melting point analysis confirmed the specificity for each of the PCR reactions. Quantitation was performed using GeneEx Macro OM 3.0 software. Primer sequences and reaction conditions for or all genes analyzed are given in Table 1. All mucin primers, and Reg3g primers were designed with Primer3 (Version 0.4.0).
Cecal loop model
For cecal loop experiments, a 50 uL overnight inoculum of C. rodentium was placed in 3 mL Dulbecco's modified eagle medium and incubated without shaking at 37°C, 5% CO2 for 3 hrs, to induce expression of the T3SS [89]. Cecal loop experiments were modified from those previously described for ileal loop experiments [90]. In brief, mice were anaesthetized by intraperitoneal injection of ketamine and xylazine. Following a midline abdominal incision, the cecum and proximal colon were gently exteriorized, and the proximal colon at the cecal-colonic junction was ligated twice. 300 uL containing approximately 1×108 cfu of pre-activated C. rodentium was then slowly injected into the cecal lumen. The cecum and colon were then returned to the abdominal cavity and the incision closed with discontinuous sutures. At given time points, the mice were euthanized and tissues collected for bacterial enumeration and histology as described above.
Bioluminescent imaging
At 4 DPI with luciferase expressing C. rodentium, mice were anaesthetized with 2% isofluorane carried in 2% O2 and imaged using an IVIS (Xenogen; Almeda, CA). Greyscale reference images taken under low illumination were collected and overlaid with images capturing the emission of photons from the lux-expressing bioluminescent C. rodentium using LIVING IMAGE software (Xenogen) and Igor (Wavemetrics; Seattle, WA). Live mice were returned to their cages.
Metabolic labeling
Metabolic labeling was carried out as previously described [52] with slight modifications. Uninfected (LB treated) and C. rodentium-infected mice were injected intraperitoneally with 20 µCi of [3H]glucosamine (Amersham) in 0.3 ml of Dulbeccos(D)-PBS (pH 7.2) and left for 3.5 hrs to metabolically label the large intestinal mucin pool. The animals were euthanized, and the colons were excised and flushed with PBS, and opened with fine scissors into a Petri dish and the mucosal surface was scraped with a glass slide to remove the adherent mucus. Mucosal secretions were placed in 15–20 ml of D-PBS and vortexed at high speed for 10 min, and then the supernatant was clarified by centrifugation (1,000 g for 10 min). The cell-free supernatant was reserved and glycoproteins were precipitated with equal volumes of 10% trichloroacetic acid (TCA) and 1% phosphotungstic acid (PTA) overnight at 4°C, solubilized in column buffer (8.06 mM Tris-HCL, 1.98 mM Tris- base, 0.001% sodium azide, pH 8.0) and neutralized to pH 7.0–7.4 with 0.1 mol/l NaOH. 5 ml of scintillation cocktail (UniverSol) was added, and 3H activity (a measure of mucus secretion) was determined in a scintillation counter. To confirm the identity of the high-molecular-weight mucin following C. rodentium infection, the secreted [3H]glucosamine-labeled glycoproteins produced in response C. rodentium and untreated controls were subjected to Sepharose-4B (Sigma) column chromatography. To do this, the 10% TCA-1% PTA-precipitated glycoproteins were dissolved in column buffer and applied to a S4B column previously equilibrated with 0.01 mol/l Tris HCl. Fractions (30–40 in total/0.4 ml each) were collected, and 3H activity of each fraction was determined by liquid scintillation counting. The results are expressed as total CPM recovered in each fraction. The column was calibrated using the following molecular weights standards: blue dextran (BD; 2,000 kDa), thyroglobulin (669 kDa) and BSA (67 kDa) (Amersham).
FITC-dextran intestinal permeability assay
This assay was performed as previously described [75]. Uninfected or infected mice at 5 DPI were gavaged with 150 µl of 80 mg/ml 4 kDa FITC-dextran (Sigma; FD4) in PBS 4 hrs prior to sacrifice. Mice were anaesthetized and blood was collected by cardiac punctures, which was added immediately to a final concentration of 3% acid-citrate dextrose (20 mM citric acid, 100 nM sodium citrate, 5 mM dextrose) (Harald Schulze, Shivdasani Laboratory, DFCI). Plasma was collected and fluorescence was quantified using a Wallace Victor (Perkin-Elmer Life Sciences, Boston, MA) at excitation 485 nm, emission 530 nm for 0.1 s.
Fluorescence in-situ hybridization
Formalin-fixed paraffin-embedded sections were deparaffinized and rehydrated as described above. Sections were incubated overnight at 37°C in the dark with Texas red-conjugated EUB338 general bacterial probe (5′-GCT GCC TCC CGT AGG AGT-3′) and an AlexaFluor 488 conjugated GAM42a probe (5′-GCC TTC CCA CAT CGT TT-3′) that recognizes bacteria that belong to the γ-Proteobacter class [58], [91] diluted to a final concentration of 2.5 ng/ul each in hybridization solution (0.9 M NaCL, 0.1 M TRIS pH 7.2, 30% Formamide, 0.1% SDS). Sections were then washed once in the dark with hybridization solution for 15 minutes with gentle shaking. This step was repeated once with wash buffer (0.9 M NaCL, 0.1 M TRIS pH 7.2), and sections were placed in dH2O, and then mounted using ProLong Gold Antifade reagent with DAPI (Molecular Probes) and imaged as described above. For quantification studies, the methods were carried as previously described [58].
SYBR green DNA staining
Large intestines were collected and prepared as described above for bacterial counts, except the lumen contents from the cecum and colon were separated. After homogenization, samples were diluted 1∶10 in PBS, then 450 ul of the 1∶10 dilution was placed in 50 ul 10% Neutral Buffered Formalin, vortexed briefly, and stored at 4¡C. 2–5 ul of the 1∶10 diluted sample stored in formalin was diluted in 1 ml PBS and filtered onto Anodisc 25 filters (Whatman International Ltd) with a pore size of 0.2 µM and 2.5 cm diameter. The samples were allowed to thoroughly dry, and then were stained with 0.25 µl SYBR green (Invitrogen) in 100 µl PBS for 15 min in the dark. Alternatively, samples were filtered onto Nucleopore Track-Etch membranes (Whatman) for DAPI staining only. The filters were air dried (in the dark for SYBR staining) and mounted on glass slides with ProLong Gold Antifade reagent with DAPI (Molecular Probes) and viewed as above. The mean number of cells counted in 3 to 6 randomly chosen fields per disc was determined.
Antimicrobial assay
Crude mucus was isolated from colorectal tissues in the same manner as described for the small intestine by by Meyer-Hoffert et al. [48]. Resected colons from WT mice were flushed gently with PBS using a pippette fitted to a syringe. Colons were then opened up longitudinally and placed in a Petri dish, mucosa side up. The round edge of forceps was then used to gently scrape off the inner colonic mucus layer with minimal damage to the epithelial surface. The mucus globule was placed in a tube, diluted 1∶1 with PBS, and mixed well by vortexing and pipetting up-and-down, and then immediately placed on ice. For the antimicrobial assay we conducted assays described by Turner et al. [92] with slight modifications. An overnight culture of streptomycin-resistant C. rodentium grown in LB was diluted 1∶1000 in Tryptic Soy Broth (TSB) and grown to mid log phase (OD620 0.6–1.0). The bacteria was washed by centrifugation (3000 rpm, 4°C, 10 mins) and removing the supernatant, and resuspending the pellet in ice cold 10 mM sodium phosphate buffer (SPB) (pH 7.4). This step was repeated once. The washed sample was diluted to a final OD620 of 0.7, diluted 1000×, and 5 uL of this dilution (containing ≈1×104 bacteria) were added to 25 ul 10 mM SPB with 0.03% TSB containing 50 ug/ml streptomycin +/−20 ul of various dilutions of crude mucin as described in the text. For negative controls, only SPB + streptomycin was added. The total reaction volume was 50 ul. Cultures were left for 3 hrs at room temp, then serially diluted and plated on LB plates containing 50 ug/ml streptomycin, and incubated overnight at 37°C incubator. Colonies were counted the next day.
Histopathological scoring
To assess tissue pathology, we used a scoring system adapted from previously described scoring systems [88], [93]. In brief, paraffin-embedded colonic tissue sections (5 µm) that had been stained with haematoxylin and eosin were examined by two blinded observers. Tissue sections were assessed for submucosal edema (0 = no change; 1 = mild; 2 = moderate; 3 = profound), epithelial hyperplasia (scored based on percentage above the height of the control where 0 = no change; 1 = 1–50%; 2 = 51–100%; 3 = >100%), epithelial integrity (0 = no change; 1 = <10 epithelial cells shedding per lesion; 2 = 11–20 epithelial cells shedding per lesion; 3 = epithelial ulceration; 4 = epithelial ulceration with severe crypt destruction); neutrophil and mononuclear cell infiltration (0 = none; 1 = mild; 2 = moderate; 3 = severe). The maximum score that could result from this scoring was 15.
Statistical analysis
Statistical significance was calculated by using either a two-tailed Student's t-test or the Mann-Whitney test unless otherwise indicated, with assistance from GraphPad Prism Software Version 4.00 (GraphPad Software, San Diego California USA, www.graphpad.com). A P value of ≤0.05 was considered significant. The results are expressed as the mean value with standard error of the mean (SEM).
Gene accession numbers
The following are the GeneIDs (Database: Entrez Gene) for each gene analyzed in this manuscript, given as gene name (official symbol GeneID #): TNF-α (Tnf GeneID: 21926); IL-23p19 (Il23a GeneID: 83430); IFN-γ (Ifng GeneID: 15978); IL-17A (Il17a GeneID: 16171), IL-17F (Il17f GeneID: 257630); IL-22 (Il22 GeneID: 50929); MCP-1 (Ccl2 GeneID: 20296); KC (Cxcl1 GeneID: 14825); iNOS (Nos2 GeneID: 18126) mCRAMP (Camp GeneID: 12796); Muc1 (Muc1 GeneID: 17829), Muc2 (Muc2 GeneID: 17831); Muc3/17 (Muc3 GeneID: 666339); Muc4 (Muc4 GeneID: 140474); Muc6 (GeneID: 353328); Muc13 (Muc13 GeneID: 17063); and Muc19 (Muc19 GeneID: 239611).
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