Factors enforcing the species boundary between the human pathogens Cryptococcus neoformans and Cryptococcus deneoformans

English version

Autoři: Shelby J. Priest ^aff001; Marco A. Coelho ^aff001; Verónica Mixão ^aff002; Shelly Applen Clancey ^aff001; Yitong Xu ^aff004; Sheng Sun ^aff001; Toni Gabaldón ^aff002; Joseph Heitman ^aff001
Působiště autorů: Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, North Carolina, United States of America ^aff001; Life Sciences Department, Barcelona Supercomputing Center, Barcelona, Spain ^aff002; Institute for Research in Biomedicine, Barcelona Institute of Science and Technology, Barcelona, Spain ^aff003; Program in Cell and Molecular Biology, Duke University Medical Center, Durham, North Carolina, United States of America ^aff004; Catalan Institution for Research and Advanced Studies (ICREA), Barcelona, Spain ^aff005
Vyšlo v časopise: Factors enforcing the species boundary between the human pathogens Cryptococcus neoformans and Cryptococcus deneoformans. PLoS Genet 17(1): e1008871. doi:10.1371/journal.pgen.1008871
Kategorie: Research Article
doi: https://doi.org/10.1371/journal.pgen.1008871

Souhrn

Hybridization has resulted in the origin and variation in extant species, and hybrids continue to arise despite pre- and post-zygotic barriers that limit their formation and evolutionary success. One important system that maintains species boundaries in prokaryotes and eukaryotes is the mismatch repair pathway, which blocks recombination between divergent DNA sequences. Previous studies illuminated the role of the mismatch repair component Msh2 in blocking genetic recombination between divergent DNA during meiosis. Loss of Msh2 results in increased interspecific genetic recombination in bacterial and yeast models, and increased viability of progeny derived from yeast hybrid crosses. Hybrid isolates of two pathogenic fungal Cryptococcus species, Cryptococcus neoformans and Cryptococcus deneoformans, are isolated regularly from both clinical and environmental sources. In the present study, we sought to determine if loss of Msh2 would relax the species boundary between C. neoformans and C. deneoformans. We found that crosses between these two species in which both parents lack Msh2 produced hybrid progeny with increased viability and high levels of aneuploidy. Whole-genome sequencing revealed few instances of recombination among hybrid progeny and did not identify increased levels of recombination in progeny derived from parents lacking Msh2. Several hybrid progeny produced structures associated with sexual reproduction when incubated alone on nutrient-rich medium in light, a novel phenotype in Cryptococcus. These findings represent a unique, unexpected case where rendering the mismatch repair system defective did not result in increased meiotic recombination across a species boundary. This suggests that alternative pathways or other mismatch repair components limit meiotic recombination between homeologous DNA and enforce species boundaries in the basidiomycete Cryptococcus species.

Klíčová slova:

Aneuploidy – Cryptococcus – Cryptococcus neoformans – Genomics – Heterozygosity – Homologous recombination – Single nucleotide polymorphisms – Genetic hybrids

Zdroje

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Článek Htt is a repressor of Abl activity required for APP-induced axonal growth

Článek Epithelial cell-turnover ensures robust coordination of tissue growth in Drosophila ribosomal protein mutants