Exocyst-mediated apical Wg secretion activates signaling in the Drosophila wing epithelium
Autoři:
Varun Chaudhary aff001; Michael Boutros aff001
Působiště autorů:
German Cancer Research Center (DKFZ), Division Signaling and Functional Genomics and Heidelberg University, Department of Cell and Molecular Biology, Im Neuenheimer Feld, Heidelberg, Germany
aff001
Vyšlo v časopise:
Exocyst-mediated apical Wg secretion activates signaling in the Drosophila wing epithelium. PLoS Genet 15(9): e32767. doi:10.1371/journal.pgen.1008351
Kategorie:
Research Article
doi:
https://doi.org/10.1371/journal.pgen.1008351
Souhrn
Wnt proteins are secreted signaling factors that regulate cell fate specification and patterning decisions throughout the animal kingdom. In the Drosophila wing epithelium, Wingless (Wg, the homolog of Wnt1) is secreted from a narrow strip of cells at the dorsal-ventral boundary. However, the route of Wg secretion in polarized epithelial cells remains poorly understood and key proteins involved in this process are still unknown. Here, we performed an in vivo RNAi screen and identified members of the exocyst complex to be required for apical but not basolateral Wg secretion. Specifically blocking the apical Wg secretion leads to reduced downstream signaling. Using an in vivo ‘temporal-rescue’ assay, our results further indicate that apically secreted Wg activates target genes that require high signaling activity. In conclusion, our results demonstrate that the exocyst is required for an apical route of Wg secretion from polarized wing epithelial cells.
Klíčová slova:
Biology and life sciences – Physiology – Physiological processes – Secretion – Genetics – Epigenetics – RNA interference – Gene expression – Genetic interference – Biochemistry – Nucleic acids – RNA – Proteins – Protein transport – Protein secretion – Cell biology – Signal transduction – Cell signaling – Signaling cascades – Wnt signaling cascade – Cell processes – Cellular types – Animal cells – Epithelial cells – Anatomy – Biological tissue – Epithelium – Molecular biology – Molecular biology techniques – Cloning – Organisms – Eukaryota – Animals – Invertebrates – Arthropoda – Insects – Drosophila – Drosophila melanogaster – Medicine and health sciences – Research and analysis methods – Animal studies – Experimental organism systems – Model organisms – Animal models
Zdroje
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