Repression of tick microRNA-133 induces organic anion transporting polypeptide expression critical for Anaplasma phagocytophilum survival in the vector and transmission to the vertebrate host

English version

Autoři: Ellango Ramasamy ^aff001; Vikas Taank ^aff001; John F Anderson ^aff002; Hameeda Sultana ^aff001; Girish Neelakanta ^aff001
Působiště autorů: Department of Biological Sciences, Old Dominion University, Norfolk, Virginia, United States of America ^aff001; Department of Entomology, Connecticut Agricultural Experiment Station, New Haven, Connecticut, United States of America ^aff002; Center for Molecular Medicine, Old Dominion University, Norfolk, Virginia, United States of America ^aff003
Vyšlo v časopise: Repression of tick microRNA-133 induces organic anion transporting polypeptide expression critical for Anaplasma phagocytophilum survival in the vector and transmission to the vertebrate host. PLoS Genet 16(7): e1008856. doi:10.1371/journal.pgen.1008856
Kategorie: Research Article
doi: https://doi.org/10.1371/journal.pgen.1008856

Souhrn

The microRNAs (miRNAs) are important regulators of gene expression. In this study, we provide evidence for the first time to show that rickettsial pathogen Anaplasma phagocytophilum infection results in the down-regulation of tick microRNA-133 (miR-133), to induce Ixodes scapularis organic anion transporting polypeptide (isoatp4056) gene expression critical for this bacterial survival in the vector and for its transmission to the vertebrate host. Transfection studies with recombinant constructs containing transcriptional fusions confirmed binding of miR-133 to isoatp4056 mRNA. Treatment with miR-133 inhibitor resulted in increased bacterial burden and isoatp4056 expression in ticks and tick cells. In contrast, treatment with miR-133 mimic or pre-mir-133 resulted in dramatic reduction in isoatp4056 expression and bacterial burden in ticks and tick cells. Moreover, treatment of ticks with pre-mir-133 affected vector-mediated A. phagocytophilum infection of murine host. These results provide novel insights to understand impact of modulation of tick miRNAs on pathogen colonization in the vector and their transmission to infect the vertebrate host.

Klíčová slova:

Bacterial pathogens – Gene expression – MicroRNAs – Nymphs – Pathogens – Ribosomal RNA – Ticks – Vertebrates

Zdroje

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