PCH-2 collaborates with CMT-1 to proofread meiotic homolog interactions
Autoři:
Stefani Giacopazzi aff001; Daniel Vong aff001; Alice Devigne aff001; Needhi Bhalla aff001
Působiště autorů:
Department of Molecular, Cell and Developmental Biology, University of California, Santa Cruz, Santa Cruz, California, United States of America
aff001
Vyšlo v časopise:
PCH-2 collaborates with CMT-1 to proofread meiotic homolog interactions. PLoS Genet 16(7): e32767. doi:10.1371/journal.pgen.1008904
Kategorie:
Research Article
doi:
https://doi.org/10.1371/journal.pgen.1008904
Souhrn
The conserved ATPase, PCH-2/TRIP13, is required during both the spindle checkpoint and meiotic prophase. However, its specific role in regulating meiotic homolog pairing, synapsis and recombination has been enigmatic. Here, we report that this enzyme is required to proofread meiotic homolog interactions. We generated a mutant version of PCH-2 in C. elegans that binds ATP but cannot hydrolyze it: pch-2E253Q. In vitro, this mutant can bind a known substrate but is unable to remodel it. This mutation results in some non-homologous synapsis and impaired crossover assurance. Surprisingly, worms with a null mutation in PCH-2’s adapter protein, CMT-1, the ortholog of p31comet, localize PCH-2 to meiotic chromosomes, exhibit non-homologous synapsis and lose crossover assurance. The similarity in phenotypes between cmt-1 and pch-2E253Q mutants suggest that PCH-2 can bind its meiotic substrates in the absence of CMT-1, in contrast to its role during the spindle checkpoint, but requires its adapter to hydrolyze ATP and remodel them.
Klíčová slova:
Apoptosis – Caenorhabditis elegans – DNA repair – Homologous chromosomes – Homologous recombination – Meiotic prophase – Nuclear staining – Synapsis
Zdroje
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