A FAS solution to a DEAD case

CshA mutant cold sensitivity is a membrane problem

In this issue of PLoS Genetics, Khemici and colleagues make the connection between two seemingly unrelated bacterial pathways, mRNA degradation and membrane biogenesis [1]. Research of the Linder lab in Geneva has long focused on the family of DEAD-box RNA helicases and their roles in RNA and DNA metabolism [2]. Here, the focus was on the Staphylococcus aureus CshA helicase, which has a general role in mRNA degradation via the RNA degradosome [3]. Khemici and colleagues used cold sensitivity of the cshA mutant to select for cold-tolerant mutant suppressors. This simple selection led to a surprising convergence of mutant genes, which mapped nearly exclusively to membrane lipid-related functions. Remarkably, most of the 82 sequenced mutations affected the fatty acid synthesis (FASII) pathway and precursor production. The authors navigated experimentally through numerous possibilities, which led them to uncover the basis for the membrane problem in cshA cold-sensitive mutants. They traced the problem to a failure to degrade the mRNA of pdh, encoding pyruvate dehydrogenase (PDH), which in aerobic conditions produces acetyl-CoA, a hub metabolite and FASII precursor (Fig 1). pdh mRNA accumulated in the cshA mutant compared to wild type (WT), reasonably predicting that acetyl-CoA pools were increased. In S. aureus, FASII uses acetyl-CoA to produce straight-chain saturated fatty acids (SCFA), which rigidify membranes. But acetyl-CoA competes with branched-chain acyl-CoA, the precursors for branched-chain fatty acids (BCFA), which fluidify membranes [4]. This SCFA to BCFA ratio is critical to membrane fluidity. Fatty acid extractions showed that SCFA to BCFA ratios were elevated in the cshA mutant compared to the WT strain. Less PDH (or more BCFA precursor production) in tested suppressor mutants restored this ratio to that of the WT, so that the membrane would regain fluidity. This highly documented study identifies pdh mRNA as the essential degradosome target for cold survival, and highlights the intimate connection between membrane state and central metabolism via acetyl-CoA.

Why are FASII genes targets for cshA suppressors?

Most cshA suppressors mapped to FASII or associated pathways. Mutations in FASII-related genes all presumably slow down phospholipid synthesis. Khemici and colleagues generalized this observation by showing that subinhibitory amounts of triclosan, which inhibits the FASII pathway protein FabI, suppressed cshA cold sensitivity. Their study also revealed that fakA, encoding a fatty acid kinase, was a cshA suppressor hotspot that corrected the SCFA to BCFA ratio. FakA depletion leads to free fatty acid accumulation that may modify S. aureus regulation and FASII activity and alter acetate utilization [5, 6]. How the slowdown of FASII synthesis adjusts fatty acid membrane composition and fluidity is a new open question arising from this work.

Do all cshA mutant suppressors relate to the SCFA to BCFA ratio and membrane fluidity?

The selection for cshA cold-tolerant suppressors identified loci seemingly unrelated to membrane biogenesis. However, underlying links may exist. For example, mutations in ndhF, encoding a membrane protein involved in the electron transport chain, would lead to reduced respiration. Consequently higher intracellular NADH might lower PDH activity, which uses NAD as cofactor. Also, suppression by mutating RNA polymerase cofactor gene rpoE may have pleiotropic effects on expression of numerous membrane-related functions [7]. Mutations that impact lipoteichoic acid production and lipoprotein maturation may not directly affect phospholipid composition but could still affect membrane fluidity. Using the SCFA to BCFA ratio as readout to understand cshA suppressors as done here will be a valuable means of characterizing these “unknown” suppressors.

New lines of exploration arising from the DEAD-membrane connection

The approach and findings reported by Khemici and colleagues provide a conceptual scaffold for discovering factors involved in the balance between central metabolism and the membrane. For example, anaerobic growth, which uses Pfl rather than Pdh to produce acetyl-CoA, might generate different conditions for cshA suppression. S. aureus incorporates exogenous unsaturated fatty acids, as enriched in host tissues [8], which might fluidify membranes and suppress cshA cold sensitivity, and idem for fluidifying solvents. Cold-sensitivity of cshA is suppressed in solid, but not liquid medium, raising the question of cell-to-cell contact, nutrient availability, and growth phase, in modulating membrane properties.

PDH and its product, acetyl-CoA, were identified here as the connecting link between cshA mutant cold survival and membrane lipid composition, as a means of adjusting membrane fluidity. Remarkably, all PDH subunits in S. aureus are prominent components of extracellular membrane vesicles [9]. Membrane blebbing could be a rapid means of removing or shuttling enzymes. Given the present study, a far-fetched but testable possibility is that PDH enrichment in extracellular vesicles might deplete intracellular PDH and favor BCFA synthesis and cell membrane fluidity. This epigenetic regulation from “without” could be an economical and rapid way to adjust membrane properties.

The membrane as a bacterial thermometer was demonstrated by several studies in the de Mendoza lab. The rigid or fluid state of membrane lipids is transmitted to membrane proteins by affecting their conformation and/or localization [10, 11]. In S. aureus, membrane fluidity affects expression of virulence factors [12]. Khemici and colleagues’ studies add CshA-degradosome turnover of pdh mRNA as another type of response to the membrane thermometer. The signal leading to pdh mRNA degradation remains to be investigated, but one possibility is that a membrane-associated degradosome component, RNase Y [13], acts as a sensor-relay of the membrane state.

The branched-chain α-keto acid dehydrogenase complex (referred to as BKD or BFM), synthesizes branched-chain acyl-CoA, the preferred competitors of acetyl-CoA in S. aureus [4]. Interestingly, BKD is a PDH complex homolog. Contrary to pdh mRNA degradation at low temperature, mRNA of the BKD analog in Bacillus subtilis is cold-stabilized [14]. It will be exciting to determine whether the S. aureus BKD complex is regulated by the degradosome under conditions where fluidity may be deleterious, e.g., at high temperature.

Altogether, this original and highly documented study brings a new clarification to the way S. aureus coordinates fundamental metabolic functions. The original conclusions and insight provided in this work will be valuable in gaining a holistic understanding of how cells respond to stress.