Centromeric SMC1 promotes centromer clustering and stabilizes meiotic homolog pairing

English version

Autoři: Talia Hatkevich ^aff001; Vincent Boudreau ^aff002; Thomas Rubin ^aff003; Paul S. Maddox ^aff002; Jean-René Huynh ^aff003; Jeff Sekelsky ^aff001
Působiště autorů: Curriculum in Genetics and Molecular Biology, University of North Carolina, Chapel Hill, North Carolina, United States of America ^aff001; Department of Biology, University of North Carolina, Chapel Hill, North Carolina, United States of America ^aff002; CIRB, Collège de France, PSL Research University, CNRS UMR7241, Inserm U1050, Paris, France ^aff003; Integrative Program in Biological and Genome Sciences, University of North Carolina, Chapel Hill, North Carolina, United States of America ^aff004
Vyšlo v časopise: Centromeric SMC1 promotes centromer clustering and stabilizes meiotic homolog pairing. PLoS Genet 15(10): e32767. doi:10.1371/journal.pgen.1008412
Kategorie: Research Article
doi: https://doi.org/10.1371/journal.pgen.1008412

Souhrn

During meiosis, each chromosome must selectively pair and synapse with its own unique homolog to enable crossover formation and subsequent segregation. How homolog pairing is maintained in early meiosis to ensure synapsis occurs exclusively between homologs is unknown. We aimed to further understand this process by examining the meiotic defects of a unique Drosophila mutant, Mcm5^A7. We found that Mcm5^A7 mutants are proficient in homolog pairing at meiotic onset yet fail to maintain pairing as meiotic synapsis ensues, causing seemingly normal synapsis between non-homologous loci. This pairing defect corresponds with a reduction of SMC1-dependent centromere clustering at meiotic onset. Overexpressing SMC1 in this mutant significantly restores centromere clustering, homolog pairing, and crossover formation. These data indicate that the initial meiotic pairing of homologs is not sufficient to yield synapsis exclusively between homologs and provide a model in which meiotic homolog pairing must be stabilized by centromeric SMC1 to ensure proper synapsis.

Klíčová slova:

Centromeres – Drosophila melanogaster – Chromosome pairs – Meiosis – Ovaries – Homologous chromosomes – Synapsis – Corolla

Zdroje

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