Droplet digitálna PCR ako nový diagnostický nástroj
Autoři:
B. Váňová 1,2; B. Malicherova 2; T. Burjanivová 3; A. Liskova 4; K. Janikova 2,5; K. Jasek 2; Z. Lasabová 3; M. Tatár 5; L. Plank 6
Působiště autorů:
Martin´s Center of Immunology, Ltd., Martin, Slovakia
1; Biomedical Center Martin JFM CU, Martin, Slovakia
2; Department of Molecular Biology JFM CU, Martin, Slovakia
3; Clinic of Obstetrics and Gynecology JFM CU and University Hospital in Martin, Slovakia
4; Department of Pathological Physiology JFM CU, Martin, Slovakia
5; Department of Pathological Anatomy, JFM CU and University Hospital in Martin, Slovakia
6
Vyšlo v časopise:
Klin Onkol 2021; 34(1): 33-39
Kategorie:
Přehled
doi:
https://doi.org/10.48095/ccko202133
Souhrn
Východiská: Podstatou moderných postupov liečby onkologických pacientov je v dnešnej dobe zacielenie konkrétnych molekúl zapojených do bunkovej signalizácie asociovanej s nádorovou iniciáciou a progresiou. Úspech uvedeného prístupu závisí od správne zvoleného diagnostického testu s vysokou citlivosťou, ktorý identifikuje výskyt a hladinu vybraných biomarkerov u pacientov pre selekciu tých, ktorí budú na liečivo reagovať a budú z neho benefitovať. Vývoj nových technológií a modernizácia tých známych, prispievajú k inováciám molekulárnej charakterizácie karcinómov, ktorá umožňuje detekciu mutačného stavu pacienta s vysokou citlivosťou a špecifickosťou. Cieľ: V práci diskutujeme o využití polymerázovej reťazovej reakcie (PCR) tretej generácie, tzv. droplet digitálnej PCR (ddPCR), v molekulárnej diagnostike karcinómov. Podľa štúdií uvedených v našom prehľade predstavuje ddPCR sľubný nástroj pri vytváraní genetického profilu pacientov s onkologickým ochorením. Optimalizácia a presná validácia môžu preto umožniť postupnú implementáciu ddPCR do klinickej praxe v oblasti onkológie.
Klíčová slova:
rakovina – nádorové biomarkery – molekulárna diagnostika – ddPCR
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