Human Rhinoviruses A9, A49, B14 and Echovirus 3, 9 among the patients with acute respiratory infection

English version

Autoři: Amir Pouremamali ¹; Manoochehr Makavndi ¹; Alireza Samarbafzadeh ²; Niloofar Neisi ¹; Mojtaba Rasti ¹; Ahmad Shamsizadeh ¹; Ali Teimoori ¹; Mehrdad Sadeghi Haj ¹; Roohangiz Nashibi ²; Shokrallah Salmanzadeh ²; Roya Nikfar ³; Rahim Soleimani ⁴; Abdolnabi Shabani ¹
Působiště autorů: Infectious Diseases Department, Medical School, Ahvaz JundiShapur University of Medical Sciences, Ahvaz, IR Iran ¹; Abuzar Children’s Hospital, Ahvaz JundiShapur University of Medical Sciences, Ahvaz, Iran ²; Infectious Diseases Department, Children Abozar Hospital, Medical School, Ahvaz JundiShapur University of Medical Sciences, Ahvaz, IR Iran ³; Department of Laboratory Sciences, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran ⁴
Vyšlo v časopise: Epidemiol. Mikrobiol. Imunol. 67, 2018, č. 1, s. 18-23
Kategorie: Původní práce

Souhrn

Background:
Acute respiratory infection result in high mortality and morbidity worldwide. There are several viral factors that originate respiratory diseases among them Enteroviruses(EVs) and Human Rhinoviruses(HRVs) can be mentioned. HRVs and EVs belong to Picornaviridae family and they have been recently classified under Enteroviruses. The pattern of respiratory infections generating organisms varies according to geographical locations. Therefore, it seems necessary to organize an appropriate plan to manage common viral diseases exclusively about Rhinoviruses and Enteroviruses.

Patient and Methods:
A total of 100 samples were collected from patients with acute respiratory infections (ARIs) who were hospitalized in Ahvaz city hospitals during December 2012 to November 2013 (one year longitude). Semi-Nested PCR was done on samples for detection of HRVs and EVs using region gene of VP4/VP2. Phylogenetic and molecular evolutionary analyses performed with MEGA version 5 software find out the sequence homology among the detected HRV and EV serotype.

Results:
The results of this study revealed that from of 100 cases of ARIs 19 patients (19%) were HRV positive and 3 (3%) patients positive for EVs. Most positive cases of HRVs were observed in the autumn season while 3 positive cases of EVs were equally found in spring, summer and autumn. Phylogenetic analyses showed that the HRV strains were HRV-A9, HRV-A49, HRV-B14 and EV strains were Echo3 and 9.

Conclusion:
The results of this study revealed that high prevalence of 19% HRVs, HRV-A9, HRV-A49, HRV-B14 serotypes and low frequency of 3% Echo Viruses, Echo3 and Echo 9 serotypes have been detected in patients with ARI.

Keywords:
Human rhinoviruses (HRVs) – Enteroviruses (EVs) –  Semi-Nested PCR – acute respiratory infections (ARIs) – Phylogenetic analysis – Khuzestan province

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