Aurora kinase A is essential for meiosis in mouse oocytes
Autoři:
Cecilia S. Blengini aff001; Patricia Ibrahimian aff001; Michaela Vaskovicova aff003; David Drutovic aff003; Petr Solc aff003; Karen Schindler aff001
Působiště autorů:
Department of Genetics; Rutgers, The State University of New Jersey, Piscataway, New Jersey, United States of America
aff001; Human Genetics Institute of New Jersey; Piscataway, New Jersey, United States of America
aff002; Institute of Animal Physiology and Genetics of the Czech Academy of Sciences, Libechov, Czech Republic
aff003
Vyšlo v časopise:
Aurora kinase A is essential for meiosis in mouse oocytes. PLoS Genet 17(4): e1009327. doi:10.1371/journal.pgen.1009327
Kategorie:
Research Article
doi:
https://doi.org/10.1371/journal.pgen.1009327
Souhrn
The Aurora protein kinases are well-established regulators of spindle building and chromosome segregation in mitotic and meiotic cells. In mouse oocytes, there is significant Aurora kinase A (AURKA) compensatory abilities when the other Aurora kinase homologs are deleted. Whether the other homologs, AURKB or AURKC can compensate for loss of AURKA is not known. Using a conditional mouse oocyte knockout model, we demonstrate that this compensation is not reciprocal because female oocyte-specific knockout mice are sterile, and their oocytes fail to complete meiosis I. In determining AURKA-specific functions, we demonstrate that its first meiotic requirement is to activate Polo-like kinase 1 at acentriolar microtubule organizing centers (aMTOCs; meiotic spindle poles). This activation induces fragmentation of the aMTOCs, a step essential for building a bipolar spindle. We also show that AURKA is required for regulating localization of TACC3, another protein required for spindle building. We conclude that AURKA has multiple functions essential to completing MI that are distinct from AURKB and AURKC.
Klíčová slova:
Immunostaining – Meiosis – Metaphase – Microtubules – Oocytes – Phosphorylation – Protein kinases – Xenopus oocytes
Zdroje
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