Rab11 activation by Ik2 kinase is required for dendrite pruning in Drosophila sensory neurons

English version

Autoři: Tzu Lin ^aff001; Hao-Hsiang Kao ^aff001; Che-Hsuan Chou ^aff001; Chih-Yu Chou ^aff001; Yu-Ching Liao ^aff001; Hsiu-Hsiang Lee ^aff001
Působiště autorů: Institute of Molecular Medicine, College of Medicine, National Taiwan University, Taipei, Taiwan ^aff001
Vyšlo v časopise: Rab11 activation by Ik2 kinase is required for dendrite pruning in Drosophila sensory neurons. PLoS Genet 16(2): e1008626. doi:10.1371/journal.pgen.1008626
Kategorie: Research Article
doi: https://doi.org/10.1371/journal.pgen.1008626

Souhrn

Neuronal pruning is a commonly observed phenomenon for the developing nervous systems to ensure precise wiring of neural circuits. The function of Ik2 kinase and its downstream mediator, Spindle-F (Spn-F), are essential for dendrite pruning of Drosophila sensory neurons during development. However, little is known about how Ik2/Spn-F signaling is transduced in neurons and ultimately results in dendrite pruning. Our genetic analyses and rescue experiments demonstrated that the small GTPase Rab11, especially the active GTP-bound form, is required for dendrite pruning. We also found that Rab11 shows genetic interactions with spn-F and ik2 on pruning. Live imaging of single neurons and antibody staining reveal normal Ik2 kinase activation in Rab11 mutant neurons, suggesting that Rab11 could have a functional connection downstream of and/or parallel to the Ik2 kinase signaling. Moreover, we provide biochemical evidence that both the Ik2 kinase activity and the formation of Ik2/Spn-F/Rab11 complexes are central to promote Rab11 activation in cells. Together, our studies reveal that a critical role of Ik2/Spn-F signaling in neuronal pruning is to promote Rab11 activation, which is crucial for dendrite pruning in neurons.

Klíčová slova:

Cloning – Drosophila melanogaster – Neuronal dendrites – Neuronal morphology – Neurons – Protein interactions – RNA interference – Guanosine triphosphatase

Zdroje

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