Poly(ADP-Ribose) Polymerase 1 (PARP-1) Regulates Ribosomal Biogenesis in Nucleoli
Poly(ADP-ribose) polymerase 1 (PARP1), a nuclear protein, utilizes NAD to synthesize poly(AD-Pribose) (pADPr), resulting in both automodification and the modification of acceptor proteins. Substantial amounts of PARP1 and pADPr (up to 50%) are localized to the nucleolus, a subnuclear organelle known as a region for ribosome biogenesis and maturation. At present, the functional significance of PARP1 protein inside the nucleolus remains unclear. Using PARP1 mutants, we investigated the function of PARP1, pADPr, and PARP1-interacting proteins in the maintenance of nucleolus structure and functions. Our analysis shows that disruption of PARP1 enzymatic activity caused nucleolar disintegration and aberrant localization of nucleolar-specific proteins. Additionally, PARP1 mutants have increased accumulation of rRNA intermediates and a decrease in ribosome levels. Together, our data suggests that PARP1 enzymatic activity is required for targeting nucleolar proteins to the proximity of precursor rRNA; hence, PARP1 controls precursor rRNA processing, post-transcriptional modification, and pre-ribosome assembly. Based on these findings, we propose a model that explains how PARP1 activity impacts nucleolar functions and, consequently, ribosomal biogenesis.
Published in the journal:
. PLoS Genet 8(1): e32767. doi:10.1371/journal.pgen.1002442
Category:
Research Article
doi:
https://doi.org/10.1371/journal.pgen.1002442
Summary
Poly(ADP-ribose) polymerase 1 (PARP1), a nuclear protein, utilizes NAD to synthesize poly(AD-Pribose) (pADPr), resulting in both automodification and the modification of acceptor proteins. Substantial amounts of PARP1 and pADPr (up to 50%) are localized to the nucleolus, a subnuclear organelle known as a region for ribosome biogenesis and maturation. At present, the functional significance of PARP1 protein inside the nucleolus remains unclear. Using PARP1 mutants, we investigated the function of PARP1, pADPr, and PARP1-interacting proteins in the maintenance of nucleolus structure and functions. Our analysis shows that disruption of PARP1 enzymatic activity caused nucleolar disintegration and aberrant localization of nucleolar-specific proteins. Additionally, PARP1 mutants have increased accumulation of rRNA intermediates and a decrease in ribosome levels. Together, our data suggests that PARP1 enzymatic activity is required for targeting nucleolar proteins to the proximity of precursor rRNA; hence, PARP1 controls precursor rRNA processing, post-transcriptional modification, and pre-ribosome assembly. Based on these findings, we propose a model that explains how PARP1 activity impacts nucleolar functions and, consequently, ribosomal biogenesis.
Introduction
The nuclear substructure, nucleolus, is a site commonly associated with translational complex assembly, and thus functions as a major regulator of cell growth [1]. The nucleolus is composed of an array of tandem repeated units of ribosomal RNA (rRNA) genes, some of which are transcribed, while others remain in an inactive heterochromatic state [2]–[4]. Additionally, the nucleolus contains a diverse pool of proteins, most of which are involved primarily with transcription, processing, and modification of rRNA transcripts, ribosome assembly, and transport of translational competent ribosome to the cytoplasm [1], [5]. Actively growing yeast cells produce about 2000 ribosomes per minute, underscoring the amount of metabolic investment made by a cell during growth towards ribosome production [6]. Ample data also suggest that the regulation of rRNA synthesis and production of ribosomes can influence cancer progression [7]. However, despite the advances in nucleolar research, the sequence of molecular events that coordinates ribosomal biogenesis with cell growth, especially in highly proliferative cells, such as cancer cells, is poorly understood.
PARP1 protein, utilizes NAD as a substrate to generate poly(ADP-ribose) (pADPr) for automodification and the modification of acceptor proteins, such as chromatin-associated histone proteins [8]–[12]. Glutamate residues of acceptor proteins serve as sites for poly(ADP-ribose) attachment [13]. Modification of proteins by PARP1 alters their localization in the cell and modifies their biological activities [14]–[17]. Since automodification disrupts the physiological activity of PARP1, it is necessary to counteract the addition of ADPr polymers. Thus, to maintain active PARP1 protein levels, ADPr polymers are removed and subsequently metabolized by PARG [18]–[21]. PARG knockout results in the accumulation of automodified PARP1, which is rendered incapable of re-associating with DNA or further catalyzing ADPr [20], [22].
Drosophila nucleoli contain large quantities of PARP1 and pADPr, and display considerable amounts of PARP1 activity [23], [24]. Whereas nucleoli structure disintegrates completely in Parp1 mutants, the ectopic expression of PARP1 cDNA restores proper assembly of nucleolar components and structure [23]. Although PARP1 does not contain any known nucleolar localization signal, it has been proposed that PARP1 localization in the nucleolus appears to depend on nucleolar activity because a large amount of PARP1 translocates from the nucleolus when ribosomal DNA (rDNA) transcription is inhibited [25], [26]. Nucleolar components, such as Fibrillarin [20], Nucleolin, and Nucleoplasmin/B23 [26], [27], interact and colocalize with PARP1 in the nucleus and undergo modification by pADPr [28]. In addition, a number of ribosomal proteins have been shown to interact with PARP1 protein [29], [30]. Both the nucleolar localization and interaction with nucleolar proteins suggest that PARP1 may function in regulating some aspect of nucleolar activity. Here we evaluate the roles of PARP1, ADPr, and nucleolar proteins that interact with PARP1 to determine the impact of PARP1 in regulating nucleolar structure and functions.
Results
Disruption of PARP1 activity causes improper localization of nucleolar-specific proteins
We previously reported that Drosophila PARP1 is broadly distributed on chromosome and is enriched in active chromatin [24]. In all tissues of wild-type Drosophila, nucleoli contained large quantities of PARP1 and pADPr, and display considerable amounts of PARP1 activity [23]. We determined that more than 40% of nuclear PARP1 (Figure 1A and 1B) and pADPr (Figure S1) are localized within the nucleolus. Since PARP1 activity within the nucleolus is not clearly defined, we investigated how its enzymatic activity affects nucleolar architecture and functions. Here we show that depletion of PARP1 protein in ParpCH1 mutant results in mis-localization of nucleolar specific proteins in all Drosophila tissues analyzed (Figure 1C and 1D). Although a large portion of nucleolar proteins shifted their localization to the cytoplasm, the total amount of these proteins did not change (Figure S2). This finding suggests that PARP1 may function in part to control nucleolar structural integrity by maintaining proteins within this subnuclear structure. To address this, we analyzed the localization of nucleolar-specific proteins under different PARP1 genetic backgrounds. We used Fibrillarin, a nucleolar protein involved in rRNA processing and maturation, as a marker for nucleolar integrity [31]. Consistent with our finding regarding ParpCH1 mutants [23], disruption of PARP1 activity by expressing ParpRNAi (Figure S3), using hypomorphic mutants ParpC03256 [32], or overproducing the antagonist of PARP1, PARG protein, caused nucleolar fragmentation, as detected by anti-Fibrillarin antibody (Figure 1E–1H). Whereas these additional nucleolus-like structures do not contain rDNA (Figure 1I–1K), they cannot take part in the ordered process of ribosomal biogenesis. Our data shows that PARP1 protein is required for nucleolar structural integrity and compromising the enzymatic activity of the protein always leads to nucleolar disruption. This finding suggests that the product of PARP1 enzymatic reaction, poly(ADP-ribose) (pADPr), may be an important component of the nucleolus and that it may serve as a matrix for nucleolar protein binding, keeping them together in proximity to precursor rRNA.
To substantiate the role of pADPr in nucleolar structure and localization of nucleolar- specific proteins, we depleted Parp1 using RNAi expression (Figure S3) and analyzed the resulting salivary gland for localization of three nucleolar-specific markers: Fibrillarin, AJ1, and nucleolar GFP-exon trap marker [33], CC01311 (Figure 2). In wild-type larvae, Fibrillarin, AJ1, and CC01311 localized in an intact single nucleolar structure (Figure 2A and 2B). However, RNAi depletion of Parp1 caused nucleolar-specific proteins to be localized completely independent from each other, unlike in wild-type tissue (Figure 2C and 2D).
The observation that depletion of PARP1 protein affected the localization of nucleolar proteins caused us to determine whether PARP1 enzymatic activity, and not PARP1 protein itself, is essential for nucleolar integrity. We inhibited the activity of PARP1 by culturing third-instar larvae in the presence of the NAD analogue, 3 aminobenzamide (3AB). Upon inhibition of PARP1 activity by 3AB, we observed the disintegration of nucleoli, as indicated by fragmented Fibrillarin (Figure 2E and 2F). A prolonged treatment of Drosophila tissues with PARP1 inhibitor exacerbates nucleolus fragmentation but does not affect localization of PARP1 protein within these nucleolus-like fragments (Figure 2G). Together, these results support the hypothesis that PARP1 enzymatic activity and pADPr are required for maintaining nucleolar structure.
Mutating PARG reveals differential localization and binding of nucleolar proteins to pADPr
To further evaluate nucleoli structural integrity, we used Transmission Electron Microscopy (TEM) analysis to detect any nucleolar abnormalities associated with disrupting PARP1 activity. TEM revealed significant changes in nucleoli in both wild-type and Parg27.1 mutant nuclei (Figure 3A and 3B). Wild-type nucleoli are typically located close to the middle part of nuclei and appear to be almost homogenic (Figure 3A). In contrast, nucleoli of Parg27.1 mutants contain heavily condensed areas positioned close to nuclear lamina (Figure 3B). Such structural changes may impact nucleolar functions and affect the steady state equilibrium of ribosomal rRNA processing. Therefore, we tested localization of nucleolar proteins reported to participate in rRNA processing and maturation in wild-type and Parg27.1 mutant nuclei using immunostaining.
In addition to Fibrillarin, we evaluated colocalization using Nucleolin and dNop5, both of which have been shown to be involved in nucleolar rRNA processing and ribosomal maturation [34], [35]. Additionally, we used Casein Kinase II α (CKIIα as a nucleolar marker, since previous reports have shown that it participates in rDNA transcription by phosphorylating components of RNA polymerase I [36]. Our results from this analysis shows that, in wild-type nucleoli, all tested nucleolar proteins demonstrate similar homogenic staining and co-localization with PARP1 protein (Figure 3C, only Fibrillarin protein is included). In contrast, nucleolar proteins in Parg27.1 nuclei accumulate in two distinct locations. This suggests that, their exclusive localization within the nucleolus requires the activity of a functional PARP1 protein.
Previously, we demonstrated that in Parg27.1 mutants, most of PARP1 protein is automodified, and, together with all other poly(ADP-ribosyl)ated proteins and pADPr binding proteins, it is either targeted to Cajal Bodies (CBs) or arrested inside abnormal condensed nucleolar substructures [20], [32]. Based on this observation, we proposed that pADPr modification would lead to co-localization with automodified PARP1 in CBs. One group of proteins, Fibrillarin and Nucleolin, co-localized with PARP1 inside condensed blocks of the nucleolus and in CBs (Figure 3D and 3E). However, another set of proteins, including nucleolar markers CC01311, dNop5 and CKIIα were preferentially antagonistic to PARP1 localization and did not accumulate in CBs (Figure 3F and 3G; Figure S4). These observations suggested a direct interaction of the first group of proteins with automodified PARP1. This interaction likely affects the location of these proteins in the nucleolus and thus may impact nucleolar activity. In contrast, CC01311, dNop5 and CKIIα do not interact with automodified PARP1. As a result, their positioning in the nucleolus is determined by PARP1-independent mechanisms. The existence of a selected group of nucleolar proteins requiring PARP1 enzymatic activity for their localization suggested to us that PARP1 may function in the nucleolus by binding these proteins through pADPr attachment.
To further test our hypothesis that a specific sub-group of nucleolar proteins preferentially interacts with pADPr, we performed co-immunoprecipitation experiments (Co-IP) using anti-pADPr antibody. Indeed, we found that Fibrillarin, AJ1, Nucleolin, and Nucleophosmin co-precipitate with pADPr in wild-type animals and Parg27.1 mutants (Figure 4A and 4B), while other nucleolar proteins, including CC01311, dNop5 and CKIIα, do not show interaction with pADPr in wild-type nuclei (Figure 4A). Interestingly, a slight interaction of CC01311 protein with pADPr was detected in Parg27.1 mutant (Figure 4B), although this weak interaction is likely indirect and may be mediated by other nucleolar components.
This result suggests that by binding a specific set of proteins to pADPr, PARP1 may determine the order of steps that occur during the process of ribosome biogenesis. Therefore, we further tested if mutating PARP1 or PARG affects any specific steps involved in ribosome production.
Inhibition of PARP1 activity disrupts rRNA processing
Resident nucleolar proteins, such as Fibrillarin, have critical roles in rRNA processing, modification, and maturation [31], [37]–[39]. Our observation of fragmented localization of nucleolar-specific proteins, some of which are critical for rRNA maturation, prompted us to investigate the effect of inhibiting PARP1 activity on rRNA processing and maturation. The transcription of rDNA produces a precursor molecule which is subjected to multiple rounds of exonucleolytic and endonucleolytic cleavages by resident nucleolar protein complexes to generate mature 18S, 5.8S, and 28S rRNA final products [40], [41]. We reasoned that the displacement of nucleolar proteins involved in rRNA processing will have a disruptive effect on the generation of mature rRNA products. To evaluate our hypothesis, we isolated RNA from both wild-type and mutant larvae and analyzed precursor rRNA intermediates using Northern blot. Surprisingly, we found no quantifiable difference between mature rRNA amounts in wild-type, Parg27.1, and ParpC03256 mutants (Figure 5, 18S and 28S). Instead, a significant increase in rRNA intermediates was detected in Parg27.1 and ParpC03256 mutants (Figure 5, Figure S5).
When taken together with the observations reported above, this suggest that displacement of key components of ribosomal biogenesis, which occurs in Parg27.1 and ParpC03256 mutants, results in rRNA processing delays and over-accumulation of immature intermediates. In contrast, the initial 47S rRNA precursor product and the intermediate 36S product were effectively processed in Parg27.1 and ParpC03256 heterozygotes, and accumulation of these two precursor products was observed in larvae lacking PARP1 or PARG function (Figure 5, red asterisks). The last finding indicates that mutating pADPr pathway does not affect the rate of rDNA transcription, but blocks specific steps of precursor rRNA maturation and accumulation of immature ribosomes. To test this hypothesis, we then proceeded to compare ribosomal content of wild-type, Parp1, and Parg mutants.
PARP1 is required for ribosome biogenesis
Our observation of rRNA intermediates processing defects in Parg27.1 and ParpC03256 larvae caused us to investigate if any changes occur in the assembly of ribosomal subunit in these mutants. To assemble competent translational machinery, mature rRNA products form complexes with accessory proteins to form the small (40S) and large (60S) subunit particles [1], [5]. These particles are released into the nucleoplasm for further maturation and then exported into the cytoplasm where they become part of the translational machinery [1]. The activity and effectiveness of the translational machinery often can be monitored by presence of multiribosomal complexes on mRNAs, called polysomes [42].
To examine the role of poly(ADP-ribosyl)ation in ribosomal production, we first compared the concentration of ribosomal particles in wild-type and Parp null mutant second-instar larvae using TEM analysis. We dissected larval midintestine from the same developmental stages of control and mutant animals, prepared ultra-thin sections from the same areas of midintestines (Figure 1C and 1D), and subjected these samples to EM analysis. Concentration of ribosomal particles was quantified using at least 5 sections for each sample. Although no difference in ribosome concentration was detected (compare Figure 6A and 6C and Figure 6B and 6D), we found a significant difference in total volume of cytoplasm between Parp null mutants and the wild-type. Cells of midintestine walls in Parp null mutant were, on average, twice as small as compared to those of the wild-type (Figure 6A and 6B, Figure S6) such phenotypes are typical of mutations within ribosome biogenesis pathways [6], [7].
We next compared ribosomal-polysomal profiles of wild-type and mutants within the pADPr turnover pathway using sucrose density gradient separation [6]. ParpCH1 mutants arrest early in development and show a phenotype similar to small second-instar larvae which limited their use in sucrose density analysis. To analyze the effect of disrupting PARP1 activity on ribosomal assembly, we used Parg27.1 and ParpC03256 mutants that survive up to late pupae. Both mutants demonstrated an absence of polysomes and abnormal quantities of mature ribosomal subunits 40S, 60S, and mono-ribosomes 80S (Figure 6E and 6F). Moreover, both mutants show a marked decline in mRNA quantity within polysomal fractions (Figure 6G), suggesting problems with mRNAs translation. Taken together with the accumulation of uncleaved rRNA intermediates (Figure 5B), these last findings suggest the presence of a significant number of aberrant misfolded and unprocessed ribosomes in the cytoplasm of Parg27.1 and ParpC03256 cells.
Discussion
Although substantial amount of PARP1 localizes in the nucleolus, prior to our study, very little was known about the function of this important protein in the nucleolus of Drosophila. We demonstrate that PARP1 activity is essential for the maintenance of Drosophila nucleolar structure and function, particularly for ribosome biogenesis. A number of nucleolar factors including Fibrillarin, AJ1, and CC01311 that co-localize in wild-type nucleolus, were observed to localize completely independent from one another when PARP1 function was disrupted. This suggests that the product of PARP1 enzymatic reaction, pADPr, may serve as a matrix for binding these nucleolar proteins and keeping them together in proximity to precursor rRNA. Our experiments with mutated PARP1 antagonist, PARG, identified a selected group of nucleolar proteins, including Fibrillarin, AJ1, Nucleolin, and Nucleophosmin, which were targeted to a specific location inside the nucleolus by PARP1 enzymatic reaction, apparently by binding of these proteins through attachment to pADPr matrix. Interestingly, although we observed a dramatic accumulation of 47S and 36S rRNA transcripts in the absence of a functional PARP1 activity, the level of 18S product was similar in both PARP1 wild-type and mutants. The accumulation of 47S and 36S rRNA transcripts can be attributed to either the upregulation of transcriptional activity in PARP1 mutants or defect in rRNA processing machinery. However, based on the dislocation of nucleolar proteins required for rRNA processing in PARP1 mutants, we believe that this accumulation is likely caused by the absence of a functional rRNA processing complex in PARP1 mutants. Furthermore, inhibiting PARP1 activity also lead to a significant reduction in the levels of ribosomes, suggesting that PARP1 activity is required for ribosome biogenesis. Taken together our findings suggest that by binding a specific set of nucleolar factors to pADPr, PARP1 likely determines the order of steps that occur during the process of ribosome biogenesis in the nucleolus.
The nucleolus is a site where the protein synthesizing machinery, the translational complex, is assembled. By virtue of this property, the nucleolus functions as a major regulator of cell growth in normal and cancer cells [43]. In addition to the proteins that make up the translational complex, the nucleolus also contains an array of proteins that function in cell cycle regulation, cell growth, and cell death induction upon exposure to DNA damaging agents [44], [45]. Findings reported here indicate that PARP1 activity is critical for nucleolar integrity and function. Recently published work by Guerrero and Maggert, support our findings that PARP1 activity is essential for the maintenance of nucleolar structure [46]. This results together with our data [23] highlights a role for PARP1 in nucleolar structure and maintenance. The research reported here extends beyond these analyses by examining PARP1 activity on the colocalization of nucleolar proteins, rRNA processing, and ribosome biogenesis. Since nucleolar function is essential during growth, this study suggests that PARP1 activity may play a central role in coordinating cell growth at the metabolic level. Here, we report our exciting observations that a novel PARP1 activity controls localization of critical components of ribosomal biogenesis within the nucleoli and therefore PARP1 is a critical regulator of ribosome production.
Transcription of ribosomal DNA in nucleoli is performed specifically by the polymerase I machinery [47]. Although this transcriptional apparatus is very different from Pol II, the presence of poly(ADP-ribose) in nucleoli suggests that transcriptional start by Pol I involves PARP1 activation as it occurs with Pol II-dependent transcription [24], [48], [49]. By summarizing our data, we could propose that upon activation of rRNA synthesis, simultaneous activation of PARP1 leads to synthesis of an equal amount of pADPr, which “attracts” proteins required for rRNA processing, modification, and loading of an initial set of ribosomal proteins. PARP1 then coordinates the steps of ribosomal maturation and protects immature ribosomes from interacting with other groups of proteins that should be loaded last (Figure 7, Figures S7 and S8). To produce poly(ADP-ribose) and regulate production of ribosomes, PARP1 utilizes a pool of NAD which is linked to energy status of the cells. Therefore, our proposed model provides a new insight into the connection between the status of metabolism of an organism and translation and cell growth. Specifically, any event leading to a decrease of NAD level in a cell should slow down all PARP1 dependent processes in ribosome biogenesis and, therefore, change the rate of translational apparatus assembly.
While our results establish a direct connection between PARP1 and ribosome biogenesis, our findings do not exclude the possibility that PARP1 accumulation has other additional functions inside the nucleolus. One such function could involve protecting genomic stability of tandemly organized clusters of ribosomal genes. The presence of tandem arrays creates a possibility of unequal crossover, as a consequence of partial loosening of rDNA, which could be crucial for viability [50]. One of the first functions proposed for PARP1 protein upon its discovery was its involvement in DNA repair [51], [52]. Therefore, PARP1 may be a specific protector of rDNA that guards it against genetic instability by creating barrier between rDNA and enzymes involved in homology repair. Alternatively, the presence of negatively charged pADPr may create a microenvironment which blocks homologue recombination within tandem arrays and therefore protects these arrays from unequal crossover.
Materials and Methods
Drosophila strains and genetics
Flies were cultured on standard cornmeal-molasses-agar media at 22°C, unless otherwise indicated. The fly stocks were generated by the standard genetic methods or obtained from the Bloomington Drosophila Stock Center and the Exelixis Collection at the Harvard Medical School, except as indicated. Genetic markers are described in Flybase [53]. CC01311 GFP-trap stock was obtained from the A. Spradling Lab [33]. The ParpC03265 strains were generated in a single pBac-element mutagenesis screen [54]. Parg27.1 [19] and ParpCH1 [23] mutants were previously described. pP{w1, UAS::PARG-EGFP}, called UAS:: PARG-EGFP, has also been previously described [21]. pP{w1, UAS::PARP1-DsRed}, called UAS::PARP1-DsRed, was described [23]. The following GAL4 driver strains were used: arm::GAL4 (Bloomington stock no. 1560), da::GAL4 (gift of A. Veraksa), and 69B-GAL4 [55]. Balancer chromosomes carrying Kr::GFP, i.e., TM3, Sb, P{w+, Kr-GFP} and FM7i, P{w1, Kr-GFP} [56], were used to identify heterozygous and homozygous ParpCH1, ParpC03265 and Parg27.1.
Construction of transgenic Drosophila
To construct the anti-Parp RNAi transgene we cloned an 1839-bp fragment of Parp-e cDNA (from GM10715 clone) in direct and inverted orientation within the pUASt vector. As a spacer between inverted repeats we used a 720-bp fragment of EGFP sequence (Figure S3). Transformation was as described [57], with modifications [58].
Western blot
Protein extracts were separated on a 4–12% gel (Invitrogen), transferred onto a nitrocellulose membrane and detected using Amersham/GE Healthcare (#RPN2106) kit, according to manufacturer's instructions. The following primary antibodies were used: anti-Fibrillarin (rabbit, 1∶4000, gift form Dr. J. Gall), anti-Nucleolin (rabbit, 1∶4000), nucleolar AJ1 antibody (rabbit, 1∶1000, gift form Dr. J. Gall), anti-Nucleophosmin (rabbit, 1∶1000), anti-dNop5 (rabbit, 1∶2000, gift form Dr. G. Vorbruggen), anti-GFP (rabbit, Torrey Pines Biolabs, #TP401, 1∶1000), anti-CKIIα (rabbit, 1∶150, Stressgen, # KAP-ST010), anti-DLG (rabbit, 1∶5000, gift from Dr, F. Roegiers) and anti-RPS6 (mouse mAb, 1∶1000, Cell Signalling #2317).
Electron microscopy
Electron microscopy was performed essentially as described [20].
Immunoprecipitation
Immunoprecipitation experiments were performed as described [20], with little modifications. Briefly, 30 ul of Protein-G Sepharose 4B were added to the protein lysates and incubated overnight at 4°C with rotation. Beads were washed 4 times for 5 min in 1 ml of lysis buffer. Bound proteins were eluted using 60 ul of 1× Laemli with heating at 95°C for 5 min. The following antibodies were used for immunoprecipitation: anti-pADPr (Mouse mAb, H10 1∶20, Tulip, #1020).
Northern blot
Total RNA was isolated using Trizol reagent (Gibco BRL), precipitated twice with 3 M LiCl, treated with Amplification Grade Dnase I (Gibco BRL). The following primers were used to produce ITS probe detecting intermediates of pre-rRNA: ITSf (5′-ataacaaaatgattccatgg-3′) and ITSr (5′-aaaaatacaccattttactgg-3′); for 18S rRNA probe: 18Sf (5′-aaaagtgaaaccgcaaaagg-3′) and 18Sr (5′-taatgatccttccccgcagg-3′). For Northern blot analysis, at least of 2.5 ug of total nuclear RNA from third instar larvae was used per lane. A Tubulin probe was used as a loading control.
Sucrose density gradient analysis
Analysis of ribosomes by sucrose density gradient centrifugation was carried out as described [6], with modifications. Briefly, 3rd instar larvae were picked, washed 2× in distilled water, followed by 20 min incubation at room temperature in 200 ul of Buffer A (20 mM HEPES [pH 7.5], 10 mM KCl, 2.5 mM MgCl2, 1 mM EGTA, 100 ug/ml cycloheximide, 1 mM DTT). 450 ul of Buffer A was then added, and larvae were then lysed on ice and centrifuged for 5 min at 10,000 rpm at 4°C. The absorbance of the supernatant was measured at 260 nm and 400 ug of each sample was carefully loaded onto a 10.5 ml 10–55% sucrose gradient in Buffer A without cycloheximide and DTT, centrifuged for 8 hrs at 27,000 rpm in a SW41 rotor. 1 ml fractions were collected using a Foxy Jr gradient collector (ISCO) with a UV detection system for recording profile. 25% of selected fractions were TCA-precipitated and analyzed by Western blot for the presence of S6 ribosomal protein (Cell Signaling). 380 ul of the remaining samples was used for RNA isolation. 30 ul of 10% SDS/Tris (pH 7.5) and 1 ml of combined phenol∶Chloroform∶Isoamyl Alcohol solution (invitrogen cat # 15593-031) was added to each sample and heated at 65°C for 2 min. Samples were centrifuged and the supernatant transferred to a new tube. Extraction was repeated again using the phenol∶Chloroform∶Isoamyl Alcohol solution, followed by transfer of the supernatant. 1/10 the volume of 3 M sodium acetate and 2× the volume of cold ethanol was added to each sample followed by incubation at −20°C for 20 min. Samples were centrifuged at 4°C for 4 min and the resulting pellet dissolved in appropriate volume of RNase free water to be used for qPCR analysis.
Quantitative Reverse Transcription–PCR
10 ug of RNA was used for cDNA synthesis using the high capacity cDNA Reverse Transcription Kit (applied biosystems cat # 4368814). The following primer sequences: Tubulin: Forward (ccttcgtccactggtacgtt), Reverse (ggcgtgacgcttagtactcc); GAPDH: Forward (cgacaagttcgtgaagctga), Reverse (attctaccgcgccctaatct); Act5C: Forward (gtgcccatctacgagggtta), Reverse (agggcaacatagcacagctt) were utilized with Power SYBER Green master mix (cat # 4367659) for PCR using the Applied Biosystems StepOnePlus detection system with the following cycling conditions: 95°C for 10 min, followed by 40 (2-step) cycles (95°C, 15 s; 60°C, 60 s).
Probes for In Situ Hybridization
rDNA probes were generated by digesting full length Drosophila rDNA gene (18S, 5.8S, and 28S) containing intergenic sequences with the following enzymes: HaeIII, AluI, MspI, RsaI, and MseI (New England Biolabs). Labeled probes were generated using DIG DNA Labeling Kit (Roche; Cat # 11 175 033 910) essentially as outlined by manufacturer.
Fluorescence In Situ Hybridization (FISH)
FISH protocol was carried out as described [59], with modifications. Salivary glands were dissected from 3rd instar larvae and fixed in for 10 min at room temperature (RT) in 4% formaldehyde in buffer A (15 mM PIPES, 80 mM KCl, 20 mM NaCl, 2 mM EDTA, 0.5 mM EGTA, 0.5 mM spermidine, 0.15 mM spermine, 1 mM DTT) prewarmed to 37°C. After fixing, salivary glands were washed three times (5 min each) in 2× SSCT (SSC in 0.1% Tween) followed by successive incubation at RT for 10 min in 20% formamide in 2× SSCT, 40% formamide in 2× SSCT, 50% formamide in 2× SSCT. Salivary glands were again incubated in fresh 50% formamide in 2× SSCT for 30 min at 37°C. The solution was aspirated and salivary glands were incubated with DNA probes diluted in 40 ul of hybridization solution (50% formamide, 3× SSCT, 20% dextran sulfate, 0.25% Tween). Probes and salivary glands were denatured (91°C for 2 min) together in a thermal cycler. Hybridization was carried out at 37°C for 24 hrs. Following hybridization, salivary glands were washed three times for 20 min each in 50% formamide in 2× SSCT at 37°C, and a wash in 25% formamide in 2× SSCT at RT. Salivary glands were washed again three times in 2× SSCT and 1× PBS/Tween at RT for 5 min each. Following washes, immunostaining protocol for Fibrillarin (outlined below) was carried out, starting by blocking with 10% BSA solution. The DIG labeled probes and Fibrillarin were detected by staining with rhodamine-conjugated anti-digoxigenin F(ab) fragments (Boehringer Mannheim) and Goat Anti-Mouse Alexa 488 diluted in 1× PBS/Tween at RT for 2 hrs. Salivary glands were washed with 1× PBS/Tween at RT twice, 20 min each time followed by incubation with DNA binding dye, Drag5, for 1 hr at RT.
Immunostaining of salivary gland
Salivary glands were dissected out in Grace's medium brought to room temperature. Salivary glands were then moved directly into fixative solution of 2% formaldehyde in PBS containing 1% Triton X-100 (PBT) (in 1.5 mL Eppendorf tube) and rotated at room temperature for 30 min. After washing twice for 5 min each in PBT, blocking solution of PBT containing 10% bovine serum albumin (10% BSA) was applied to salivary glands and rotated at room temperature for 1 hr. After blocking in 10% BSA solution, salivary glands were washed in PBT containing 1% bovine serum albumin (1% BSA) for 5 min. Primary antibodies were then applied to salivary glands. Mouse Anti-Nop1p Fibrillarin (Corning) at a dilution of 1∶200, Rabbit Anti-Nucleolin (Abcam) at a dilution of 1∶200, Rabbit Anti-Fibrillarin (Abcam) at a dilution of 1∶500, Rabbit Anti-dNop5 (1∶400), Rabbit nucleolar AJ1 antibody (1∶400) and Rabbit Anti-GFP (1∶500) were applied to respective samples. Salivary glands were incubated in primary antibody overnight at 4 degrees on a rotator. After that, samples were washed in 1% BSA solution three times for 10 min each. Salivary glands were incubated with appropriate secondary antibody at room temperature on rotator for 2 hrs, and Goat Anti-Mouse Alexa 488, Goat Anti-Rabbit Alexa 568, Goat Anti-Rabbit Alexa 488 and Alexa 633 (from Molecular Probes) at a dilution of 1∶400 were applied. Next, samples were washed twice in PBT buffer for 5 min and then subjected to chromatin staining using Draq5 (Biostatus) at a dilution 1∶500 in PBT buffer for 1 hr at room temperature on rotator or Oligreen (Invitrogen) at a dilution of 1∶10,000 in PBT buffer solution for 10 min at room temperature. Salivary glands stained with Oligreen were then washed twice for 5 min in PBT buffer solution and fixed to microscope slide. Images were obtained using the Leica (DM-IRB) Confocal System.
Supporting Information
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Štítky
Genetika Reprodukční medicínaČlánek vyšel v časopise
PLOS Genetics
2012 Číslo 1
- Primární hyperoxalurie – aktuální možnosti diagnostiky a léčby
- Srdeční frekvence embrya může být faktorem užitečným v předpovídání výsledku IVF
- Akutní intermitentní porfyrie
- Vztah užívání alkoholu a mužské fertility
- Šanci na úspěšný průběh těhotenství snižují nevhodné hladiny progesteronu vznikající při umělém oplodnění
Nejčtenější v tomto čísle
- Poly(ADP-Ribose) Polymerase 1 (PARP-1) Regulates Ribosomal Biogenesis in Nucleoli
- Microenvironmental Regulation by Fibrillin-1
- Parallel Mapping and Simultaneous Sequencing Reveals Deletions in and Associated with Discrete Inherited Disorders in a Domestic Dog Breed
- Two-Component Elements Mediate Interactions between Cytokinin and Salicylic Acid in Plant Immunity