Molekulární a antigenní analýza hemaglutininu virů chřipky A H3N2 detekovaných v České republice v epidemické sezoně 2014/2015

English version

Autoři: A. Nagy ^1,2; H. Jiřincová ²; J. Kynčl ^3,4 ; M. Havlíčková ²
Působiště autorů: Laboratory of Molecular Methods, State Veterinary Institute Prague, Czech Republic ¹; National Reference Laboratory for Influenza, National Institute of Public Health, Prague, Czech Republic ²; Department of Infectious Diseases Epidemiology, National Institute of Public Health, Prague, Czech Republic ³; Department of Epidemiology, Third Faculty of Medicine, Charles University in Prague, Czech Republic ⁴
Vyšlo v časopise: Epidemiol. Mikrobiol. Imunol. 65, 2016, č. 2, s. 92-101
Kategorie: Původní práce

Souhrn

Epidemická sezona 2014/2015 byla charakterizována dominancí subtypu H3N2. Cílem této studie je analýza genetické a antigenní heterogenity kmenů H3N2 detekovaných v České republice v období listopad 2014 až březen 2015 na úrovni molekuly hemaglutininu (HA) H3. Za tímto účelem byla provedena fylogenetická analýza reprezentativních sekvencí H3 HA a glykosylační status a klíčové antigenní mutace byly porovnány s vakcinálními kmeny pro roky 2014 a 2015 (A/Texas/50/2012 a A/Switzerland/9715293/2013) a následně vizualizovány v krystalografické struktuře proteinu H3. Molekulární analýza byla doplněna o výsledky hemaglutinačně inhibičního testu (HIT) s patnácti H3N2 kmeny s využitím antisér A/Texas/50/2012 a A/Switzerland/9715293/2013. Výsledky analýzy českých H3N2 kmenů cirkulujících v sezoně 2014/2015 slouží jako doplnění výsledů oficiálních institucí o data z konkrétní geografické oblasti.

KLÍČOVÁ SLOVA:
influenza – chřipka – H3N2 – hemaglutinin

Zdroje

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