Fluorescence in situ hybridization on histologic sections

Česká verze

Authors: Marcela Mrhalová; Roman Kodet
Authors place of work: Ústav patologie a molekulární medicíny 2. LF UK a FN v Motole, Praha
Published in the journal: Čes.-slov. Patol., 49, 2013, No. 4, p. 114-122
Category: Přehledové články – Molekulární diagnostika

Summary

I-FISH (fluorescence in situ hybridization on interphasic nuclei) represents a laboratory method linking morphological investigations (histological sections of formaldehyde fixed and paraffin embedded tissues) with molecular techniques (sequence specificity of nucleic acids bases for a certain locus). I-FISH is relatively undemanding for a laboratory workout, but offering a lot of important information about the investigated cells. Within a scope of pathology departments I-FISH is utilized mostly in diagnostics of neoplasms. I-FISH is helpful in detecting gene copy numbers (amplifications or deletions), and, importantly, in establishing copy numbers of individual chromosomes (polysomies or monosomies), chromosomal breaks and translocations. At present, I-FISH is used not only for diagnosis and estimation of prognosis, but also as a method to qualify a patient for a targeted biological therapy. Because demands on investigation of solid tumors keep raising I-FISH becomes a part of routine investigations. The aim of this paper is to summarize principles and the utility of I-FISH and to help the interested readers in finding a basic orientation in this laboratory method.

Keywords:
fluorescence in situ hybridization – histological section – fluorescence microscope – probe – interphasic nuclei – formaldehyde fixation

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