Determination of body fluid based on analysis of nucleic acids

Česká verze

Authors: Marie Korabečná ^1,2
Authors place of work: Ústav biologie a lékařské genetiky 1. lékařské fakulty UK a Všeobecné fakultní nemocnice, Praha ¹; Lékařská fakulta UK v Plzni, Plzeň ²
Published in the journal: Soud Lék., 60, 2015, No. 3, p. 33-36
Category: Přehledový článek

Summary

Recent methodological approaches of molecular genetics allow isolation of nucleic acids (DNA and RNA) from negligible forensic samples. Analysis of these molecules may be used not only for individual identification based on DNA profiling but also for the detection of origin of the body fluid which (alone or in mixture with other body fluids) forms the examined biological trace. Such an examination can contribute to the evaluation of procedural, technical and tactical value of the trace. Molecular genetic approaches discussed in the review offer new possibilities in comparison with traditional spectrum of chemical, immunological and spectroscopic tests especially with regard to the interpretation of mixtures of biological fluids and to the confirmatory character of the tests. Approaches based on reverse transcription of tissue specific mRNA and their subsequent polymerase chain reaction (PCR) and fragmentation analysis are applicable on samples containing minimal amounts of biological material. Methods for body fluid discrimination based on examination of microRNA in samples provided so far confusing results therefore further development in this field is needed. The examination of tissue specific methylation of nucleotides in selected gene sequences seems to represent a promising enrichment of the methodological spectrum. The detection of DNA sequences of tissue related bacteria has been established and it provides satisfactory results mainly in combination with above mentioned methodological approaches.

Keywords:
body fluids – mRNA – microRNA – qPCR– promotermethylation – microbial DNA

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