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Possibilities for the analysis of peripheral blood B cell subpopulations in a routine immunological laboratory


Authors: J. Štíchová 1;  J. Nechvátalová 1,2;  J. Litzman 1,2;  M. Vlková 1,2
Authors place of work: Ústav klinické imunologie a alergologie, LF MU v Brně 1;  Ústav klinické imunologie a alergologie, Fakultní nemocnice u sv. Anny v Brně, Brno 2
Published in the journal: Epidemiol. Mikrobiol. Imunol. 70, 2021, č. 4, s. 264-280
Category: Souhrnné sdělení

Summary

B cells play a vital role in the defence of the body against infectious agents. Apart from their ability to present antigen to T cells, B cells are mainly producers of antibodies. These play a crucial role in the effective elimination of infection and are also involved in the regulation of the immune response. The analysis of peripheral blood B cell subpopulations that makes it possible to monitor the development of B cells to the stage of antibody producing plasmablasts provides a valuable laboratory parameter which is important for both the study of the pathogenesis and diagnosis of some diseases. Laboratory analysis of B cell subpopulations is now a routinely available laboratory option thanks to the development of multicolour flow cytometry. This article summarizes the core knowledge which is currently applied to the analysis of B cell subpopulations in immunological laboratories.

Keywords:

B cell– flow cytometry – marginal zone of the spleen – germinal centre


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Hygiena a epidemiologie Infekční lékařství Mikrobiologie

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