Hodnocení aktivace lymfocytů sledováním expresečasného aktivačního antigenu CD69 průtokovoucytometrií u pacientů na JIP

Evaluation of Activation of Lymphocytesby Determining Expression of Early Activation Antigen CD 69 of Patientson ICU by Flow Cytometry

Disorders in immune reactions represent one of the main pathogenetic mechanisms in patients ofIntensive Care Units who suffer from sepsis or syndrome of systemic inflammatory response. Thedetermination of early activation sign CD69 on T lymphocytes makes it possible to evaluatefunctional capacity of this cell population. The determination of other selected immunologicalparameters contributes to differential diagnosis of infectious andnon-infectious etiology of systemicinflammatory response. The authors demonstrated lower expression of CD69 on CD3+CD8+ lymphocytesafter stimulation with phytohemagglutinin in patients with sepsis as well as patients with thesyndrome of systemic inflammatory response of non-infections etiology. No statistically significantdifferences were proved in the concentrations of IgG, IgA and IgM,number of leukocytes, percentualrepresentation of lymphocytes, TNFalpha production and the expression of surface sign of CD64 inboth groups of patients. There were statistically significant differences in the concentrations of C3and C4 components of complement, prealbumin and procalcitonin. The study did not show correlationbetween the expression of CD69 and selected immunological parameters (IgA, IgM, IgG, C3, C4,leukocytes, lymphocytes, prealbumin, procalcitonin, TNFalpha and CD64).

Key words:
sepsis – syndrome of systemic inflammatory response – functional test of proliferationof lymphocytes – immunological diagnostics.