Online solid phase extraction analytical system coupled to tandem mass spectrometry for clinical applications.
Authors:
I. Vrobel 1,2; D. Friedecký 1,2; T. Adam 1,2
Authors‘ workplace:
Ústav molekulární a translační medicíny, Univerzita Palackého v Olomouci
1; Oddělení klinické biochemie, Fakultní nemocnice a Univerzita Palackého v Olomouci
2
Published in:
Klin. Biochem. Metab., 26, 2018, No. 1, p. 11-17
Overview
Objective:
Introduction to the online solid-phase extraction system coupled to tandem mass spectrometry (SPE-MS/MS). Assessment of the suitability of this system for clinical laboratories as an alternative to liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS).
Methods:
Characterization of functional principle of the online SPE-MS/MS system. Description of crucial parameters that has to be followed and evaluated during method development. Brief summary of so-far published methods based on utilization of this technique from the fields of toxicology, therapeutic drug monitoring and clinical biochemistry.
Results and discussion:
The online SPE-MS/MS system enables fast determination of analytes in common biological matrices. The system ensures extensive cleaning of the sample. High sensitivity is provided by utilization of multiple reaction monitoring (MRM) mode in tandem mass spectrometer. The analysis of one sample takes usually around 15 sec. This leads to significant speeding of the analytical process in comparison to methods based on LC-MS/MS. At the same time all requirements for validation of bioanalytical methods are met. The key parameters assessed during method development process are the matrix effect, carry-over, potential isobaric interferences and fragmentation of labile conjugates in the ion source.
Conclusion:
The online SPE-MS/MS system can be used advantageously in the range of clinical applications, particularly in the case of exogenous analytes occurring in the sample at relatively high concentrations. Nevertheless, this system cannot be exploited for all types of analytes. In many cases, it can, however, significantly speed up and cheapen the analytical process and suitably complement the LC-MS/MS systems.
Keywords:
SPE-MS/MS, RapidFire, mass spectrometry, solid phase extraction, quantitative analysis.
Sources
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Clinical biochemistry Nuclear medicine Nutritive therapistArticle was published in
Clinical Biochemistry and Metabolism
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