The phage gene wmk is a candidate for male killing by a bacterial endosymbiont
Autoři:
Jessamyn I. Perlmutter aff001; Sarah R. Bordenstein aff001; Robert L. Unckless aff003; Daniel P. LePage aff001; Jason A. Metcalf aff001; Tom Hill aff003; Julien Martinez aff005; Francis M. Jiggins aff005; Seth R. Bordenstein aff001
Působiště autorů:
Department of Biological Sciences, Vanderbilt University, Nashville, Tennessee, United States of America
aff001; Vanderbilt Microbiome Initiative, Vanderbilt University, Nashville, Tennessee, United States of America
aff002; Department of Molecular Biosciences, University of Kansas, Lawrence, Kansas, United States of America
aff003; Department of Pediatrics, University of Michigan, Ann Arbor, Michigan, United State of America
aff004; Department of Genetics, University of Cambridge, Cambridge, United Kingdom
aff005; Department of Pathology, Microbiology, and Immunology, Vanderbilt University, Nashville, Tennessee, United States of America
aff006; Vanderbilt Institute for Infection, Immunology and Inflammation, Vanderbilt University, Nashville, Tennessee, United States of America
aff007
Vyšlo v časopise:
The phage gene wmk is a candidate for male killing by a bacterial endosymbiont. PLoS Pathog 15(9): e32767. doi:10.1371/journal.ppat.1007936
Kategorie:
Research Article
doi:
https://doi.org/10.1371/journal.ppat.1007936
Souhrn
Wolbachia are the most widespread maternally-transmitted bacteria in the animal kingdom. Their global spread in arthropods and varied impacts on animal physiology, evolution, and vector control are in part due to parasitic drive systems that enhance the fitness of infected females, the transmitting sex of Wolbachia. Male killing is one common drive mechanism wherein the sons of infected females are selectively killed. Despite decades of research, the gene(s) underlying Wolbachia-induced male killing remain unknown. Here using comparative genomic, transgenic, and cytological approaches in fruit flies, we identify a candidate gene in the eukaryotic association module of Wolbachia prophage WO, termed WO-mediated killing (wmk), which transgenically causes male-specific lethality during early embryogenesis and cytological defects typical of the pathology of male killing. The discovery of wmk establishes new hypotheses for the potential role of phage genes in sex-specific lethality, including the control of arthropod pests and vectors.
Klíčová slova:
Biology and life sciences – Developmental biology – Embryology – Embryos – Organisms – Bacteria – Wolbachia – Eukaryota – Animals – Invertebrates – Arthropoda – Insects – Drosophila – Drosophila melanogaster – Viruses – Bacteriophages – Population biology – Population metrics – Sex ratio – Genetics – Phenotypes – Genomics – Comparative genomics – Gene expression – Computational biology – Research and analysis methods – Animal studies – Experimental organism systems – Model organisms – Animal models
Zdroje
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